A hypersonic aerodynamics analysis of an electromagnetic gun(EM gun) launched projectile configuration is undertaken in order to ameliorate the basic aerodynamic characteristics in comparison with the regular projecti...A hypersonic aerodynamics analysis of an electromagnetic gun(EM gun) launched projectile configuration is undertaken in order to ameliorate the basic aerodynamic characteristics in comparison with the regular projectile layout.Static margin and pendulum motion analysis models have been applied to evaluate the flight stability of a new airframe configuration.With a steady state computational fluid dynamics(CFD) simulation,the basic density,pressure and velocity contours of the EM gun projectile flow field at Mach number 5.0,6.0 and 7.0(angle of attack=0°) have been analyzed.Furthermore,the static margin values are enhanced dramatically for the EM gun projectile with configuration optimization.Drag,lift and pitch property variations are all illustrated with the changes of Mach number and angle of attack.A particle ballistic calculation was completed for the pendulum analysis.The results show that the configuration optimized projectile,launched from the EM gun at Mach number 5.0 to 7.0,acts in a much more stable way than the projectiles with regular aerodynamic layout.展开更多
Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely st...Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely studied model system for Coronavirus replication and pathogenesis. In this study,we created a MHV-A59 temperature sensitive (ts) mutant Wu"-ts18(cd) using the recombinant vaccinia reverse genetics system. Virus replication assay in 17C1-1 cells showed the plaque phenotype and replication characterization of constructed Wu"-ts18(cd) were indistinguishable from the reported ts mutant Wu"-ts18. Then we cultured the ts mutant Wu"-ts18(cd) at non-permissive temperature 39.5°C,which "forced" the ts recombinant virus to use second-site mutation to revert from a ts to a non-ts phenotype. Sequence analysis showed most of the revertants had the same single amino acid mutation at Nsp16 position 43. The single amino acid mutation at Nsp16 position 76 or position 130 could also revert the ts mutant Wu"-ts18 (cd) to non-ts phenotype,an additional independent mutation in Nsp13 position 115 played an important role on plaque size. The results provided us with genetic information on the functional determinants of Nsp16. This allowed us to build up a more reasonable model of CoVs replication-transcription complex.展开更多
In order to establish the eukaryotic cell lines for inducible control of SARS-CoV nucleocapsid gene expression.The recombinant plasmid of pTRE-Tight-SARS-N was constructed by using the plasmid p8S as the PCR template ...In order to establish the eukaryotic cell lines for inducible control of SARS-CoV nucleocapsid gene expression.The recombinant plasmid of pTRE-Tight-SARS-N was constructed by using the plasmid p8S as the PCR template which contains a cDNA clone covering the nucleocapsid gene of SARS-CoV HKU-39449. Restriction enzymes digestion and sequence analysis indicated the recombinant plasmid of pTRE-Tight-SARS-N contained the nucleocapsid gene with the optimized nucleotide sequence which will improve the translation efficiency. Positive cell clones were selected by cotransfecting pTRE-Tight-SARS-N with the linear marker pPUR to BHK-21 Tet-on cells in the presence of puromycin. A set of double-stable eukaryotic cell lines (BHK-Tet-SARS-N) with inducible control of the SARS-CoV neucleocapsid gene expression was identified by using SDS-PAGE and Western-blot analysis. The expression of SARS-CoV nucleocapsid protein was tightly regulated by the varying concentration of doxcycline in the constructed double-stable cell line. The constructed BHK-Tet-SARS-N cell strains will facilitate the rescue of SARS-CoV in vitro and the further reverse genetic research of SARS-CoV.展开更多
基金supported by Youth Science and Technology Research FundShanxi Province Applied Basic Research Projectgrant number 201801D221039+2 种基金Science Foundation of North University of China grant number XJJ201813Scientific and Technologial Innovation Programs of Higher Education Institutions in Shanxi grant number 2019L0570Aeronautical Science Foundation of China grant number 2019020U0002。
文摘A hypersonic aerodynamics analysis of an electromagnetic gun(EM gun) launched projectile configuration is undertaken in order to ameliorate the basic aerodynamic characteristics in comparison with the regular projectile layout.Static margin and pendulum motion analysis models have been applied to evaluate the flight stability of a new airframe configuration.With a steady state computational fluid dynamics(CFD) simulation,the basic density,pressure and velocity contours of the EM gun projectile flow field at Mach number 5.0,6.0 and 7.0(angle of attack=0°) have been analyzed.Furthermore,the static margin values are enhanced dramatically for the EM gun projectile with configuration optimization.Drag,lift and pitch property variations are all illustrated with the changes of Mach number and angle of attack.A particle ballistic calculation was completed for the pendulum analysis.The results show that the configuration optimized projectile,launched from the EM gun at Mach number 5.0 to 7.0,acts in a much more stable way than the projectiles with regular aerodynamic layout.
基金Research Grants from State Key Laboratory of Pathogen and Biosecurity (SKLPBS0918)
文摘Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely studied model system for Coronavirus replication and pathogenesis. In this study,we created a MHV-A59 temperature sensitive (ts) mutant Wu"-ts18(cd) using the recombinant vaccinia reverse genetics system. Virus replication assay in 17C1-1 cells showed the plaque phenotype and replication characterization of constructed Wu"-ts18(cd) were indistinguishable from the reported ts mutant Wu"-ts18. Then we cultured the ts mutant Wu"-ts18(cd) at non-permissive temperature 39.5°C,which "forced" the ts recombinant virus to use second-site mutation to revert from a ts to a non-ts phenotype. Sequence analysis showed most of the revertants had the same single amino acid mutation at Nsp16 position 43. The single amino acid mutation at Nsp16 position 76 or position 130 could also revert the ts mutant Wu"-ts18 (cd) to non-ts phenotype,an additional independent mutation in Nsp13 position 115 played an important role on plaque size. The results provided us with genetic information on the functional determinants of Nsp16. This allowed us to build up a more reasonable model of CoVs replication-transcription complex.
基金This work was supported by the European Commission (SARS-DTV ) SP22-CT-2004–511064)the State Key Laboratory of Pathogen and Biosecunity SKLPBS0918
文摘In order to establish the eukaryotic cell lines for inducible control of SARS-CoV nucleocapsid gene expression.The recombinant plasmid of pTRE-Tight-SARS-N was constructed by using the plasmid p8S as the PCR template which contains a cDNA clone covering the nucleocapsid gene of SARS-CoV HKU-39449. Restriction enzymes digestion and sequence analysis indicated the recombinant plasmid of pTRE-Tight-SARS-N contained the nucleocapsid gene with the optimized nucleotide sequence which will improve the translation efficiency. Positive cell clones were selected by cotransfecting pTRE-Tight-SARS-N with the linear marker pPUR to BHK-21 Tet-on cells in the presence of puromycin. A set of double-stable eukaryotic cell lines (BHK-Tet-SARS-N) with inducible control of the SARS-CoV neucleocapsid gene expression was identified by using SDS-PAGE and Western-blot analysis. The expression of SARS-CoV nucleocapsid protein was tightly regulated by the varying concentration of doxcycline in the constructed double-stable cell line. The constructed BHK-Tet-SARS-N cell strains will facilitate the rescue of SARS-CoV in vitro and the further reverse genetic research of SARS-CoV.
