目的研究Gli表达下调对食管腺癌OE33细胞生长转移的影响,并探讨其相关的分子机制。方法将Gli1、Gli2 si RNA和空白对照si RNA转染OE33细胞48 h,实时荧光定量聚合酶链反应检测OE33细胞Gli1和Gli2 m RNA表达水平;采用Western blot检测OE3...目的研究Gli表达下调对食管腺癌OE33细胞生长转移的影响,并探讨其相关的分子机制。方法将Gli1、Gli2 si RNA和空白对照si RNA转染OE33细胞48 h,实时荧光定量聚合酶链反应检测OE33细胞Gli1和Gli2 m RNA表达水平;采用Western blot检测OE33细胞中Gli1、Gli2、Cyclin D1、p27、E-cadherin及N-cadherin蛋白的表达水平;流式细胞术检测OE33细胞增殖和细胞周期分布;Transwell实验检测细胞侵袭能力。结果采用Gli1和Gli2 si RNA抑制Gli1和Gli2 m RNA及蛋白表达后,可下调Cyclin D1和N-cadherin蛋白表达,增加E-cadherin和p27蛋白表达,同时抑制细胞增殖,将细胞周期阻滞在G_0/G_1期并降低细胞的侵袭能力,与对照组比较,差异有统计学意义(P<0.05)。结论 Gli在食管腺癌的生长转移中具有重要作用,敲掉Gli1和Gli2基因表达可抑制食管腺癌细胞周期分布和上皮-间质转化能力,这可能与Cyclin D1、p27、E-cadherin及N-cadherin蛋白变化相关。展开更多
The relationships of cooling rate with microstructure and thermal conductivity of vermicular graphite cast iron(VGI) cylinder block were studied, which are important for design and optimization of the casting process ...The relationships of cooling rate with microstructure and thermal conductivity of vermicular graphite cast iron(VGI) cylinder block were studied, which are important for design and optimization of the casting process of VGI cylinder blocks. Cooling rates at different positions in the cylinder block were calculated based on the cooling curves recorded with a solidification simulation software. The metallographic structure and thermal conductivity were observed and measured using optical microscopy(OM), scanning electrical microscopy(SEM) and laser flash diffusivity apparatus, respectively. The effects of the cooling rate on the vermicularity, total and average areas of all graphite particles, and the pearlite fraction in the VGI cylinder block were investigated. It is found that the vermicularity changes in parabola trend with the increase of cooling rate. The total area of graphite particles and the cooling rate at eutectoid stage can be used to predict pearlite fraction well. Moreover, it is found that the thermal conductivity at room temperature is determined by the average area of graphite particles and pearlite fraction when the range of vermicularity is from 80% to 93%. Finally, the quantitative models are established to calculate the vermicularity, pearlite fraction, and thermal conductivity of the VGI cylinder block.展开更多
文摘目的研究Gli表达下调对食管腺癌OE33细胞生长转移的影响,并探讨其相关的分子机制。方法将Gli1、Gli2 si RNA和空白对照si RNA转染OE33细胞48 h,实时荧光定量聚合酶链反应检测OE33细胞Gli1和Gli2 m RNA表达水平;采用Western blot检测OE33细胞中Gli1、Gli2、Cyclin D1、p27、E-cadherin及N-cadherin蛋白的表达水平;流式细胞术检测OE33细胞增殖和细胞周期分布;Transwell实验检测细胞侵袭能力。结果采用Gli1和Gli2 si RNA抑制Gli1和Gli2 m RNA及蛋白表达后,可下调Cyclin D1和N-cadherin蛋白表达,增加E-cadherin和p27蛋白表达,同时抑制细胞增殖,将细胞周期阻滞在G_0/G_1期并降低细胞的侵袭能力,与对照组比较,差异有统计学意义(P<0.05)。结论 Gli在食管腺癌的生长转移中具有重要作用,敲掉Gli1和Gli2基因表达可抑制食管腺癌细胞周期分布和上皮-间质转化能力,这可能与Cyclin D1、p27、E-cadherin及N-cadherin蛋白变化相关。
文摘The relationships of cooling rate with microstructure and thermal conductivity of vermicular graphite cast iron(VGI) cylinder block were studied, which are important for design and optimization of the casting process of VGI cylinder blocks. Cooling rates at different positions in the cylinder block were calculated based on the cooling curves recorded with a solidification simulation software. The metallographic structure and thermal conductivity were observed and measured using optical microscopy(OM), scanning electrical microscopy(SEM) and laser flash diffusivity apparatus, respectively. The effects of the cooling rate on the vermicularity, total and average areas of all graphite particles, and the pearlite fraction in the VGI cylinder block were investigated. It is found that the vermicularity changes in parabola trend with the increase of cooling rate. The total area of graphite particles and the cooling rate at eutectoid stage can be used to predict pearlite fraction well. Moreover, it is found that the thermal conductivity at room temperature is determined by the average area of graphite particles and pearlite fraction when the range of vermicularity is from 80% to 93%. Finally, the quantitative models are established to calculate the vermicularity, pearlite fraction, and thermal conductivity of the VGI cylinder block.
