Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber(Cucumis sativus L.)

P450(cytochrome P450)is a supergene family,which is involved in various metabolic pathways in plants.Based on previous study,we found some of cucumber P450 mRNAs were systemic mobile in cucumber/pumpkin grafts.However,the reason that why P450 mRNAs were endorsed as signaling,and what specific motif(s)did they harbored is not clear yet.Here,we first identified 221 CsaP450 genes in cucumber genomewide level.Combining with graft-transmissiblemRNAs datasets in cucumber,we elucidated 15mobile-CsaP450-coding genes,of which 5 and 10 belonged to A-type and non-A type respectively.Compared with Arabidopsis and pumpkin(Cucubit moschata)graft-induced-transmissible P450 mRNAs,a phylogenetic treewas constructed and divided into eight clans by usingmultiple-sequence alignment.Gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)annotations indicated that the expression patterns of the mobile-mRNA-coding CsaP450 genes in different tissues of cucumber was specifically enriched in oxidoreductase activity and secondary metabolic pathways.The structures and motifs of these 15 mobile-mRNA-coding CsaP450 genes and their types of regulatory elements told that the proportion of CU-rich motifs was higher than nonmobile-mRNA-coding CsaP450 genes.The integrated analysis of mobility direction and mRNA abundance of 15 mobilemRNA-coding CsaP450 genes allowed to conclude that there was rarely relationship between them.The study provided a new insight into the relationship between the motifs and functional characterization of mobile-mRNA-coding P450 genes of cucumber in genome-wide levels.

Xiongzhi Dilong decoction interferes with calcitonin gene-related peptide(CGRP)-induced migraine in rats through the CGRP/iNOS pathway

Objective: To evaluate the effects of Xiongzhi Dilong decoction(XZDLD) and its wind medicine on calcitonin gene-related peptide(CGRP)-induced migraine and explore the mechanism through the CGRP/inducible nitric oxide synthase(iNOS) pathway.Methods: Rats were divided into control, model, XZDLD, XZDLD(external wind), XZDLD(internal wind),and olcegepant groups. CGRP was injected into the dura mater to induce a migraine. The frequency of head scratching, cage climbing, and facial grooming was observed. The pain threshold, the levels of CGRP,pituitary adenylate cyclase activating polypeptide(PACAP), substance P(SP), and the plasma protein extravasation(PPE) ratio were measured. The phosphorylation levels of p38, extracellular signalregulated kinase 1/2(ERK1/2), and expression of iNOS were detected by western blot.Results: Compared with the model group, the three modified XZDLD groups showed reduced frequency of head scratching and cage climbing in the first 30 min(all P <.05). Facial grooming frequency was reduced in XZDLD and XZDLD(external wind) groups(P =.0003 and P =.0131, respectively). External wind medicine played a more important role in increasing mechanical pain threshold than internal wind medicine. Moreover, compared with the model group, the three modified XZDLD groups demonstrated reduced plasma levels of CGRP and PACAP(all P <.05). No difference in the SP level was observed among the six groups. XZDLD reduced PPE ratio. XZDLD and XZDLD(external wind) groups suppressed the CGRP/iNOS pathway by inhibiting the p-p38/p38 ratio and the expression of iNOS. No difference in pERK1/2/ERK1/2 ratio was detected among the six groups.Conclusion: XZDLD increases pain threshold, downregulates the expression of CGRP and PACAP, and reduces PPE ratio by inhibiting the CGRP/iNOS pathway. External wind medicine is more effective than internal one on improving facial grooming and head scratching, increasing the mechanical pain threshold, and inhibiting the expression of iNOS.

Putting the Cart before the Horse? Capital Account Liberalization and Exchange Rate Flexibility in China

This paper reviews the issues involved in moving towards greater exchange rate flexibilityand capital account liberalization in China. A more flexible exchange rate regime wouldallow China to operate a more independent monetary policy, providing a useful bufferagainst domestic and external shocks. At the same time, weaknesses in China’s financialsystem suggest that capital account liberalization poses significant risks and should be alower priority in the short term. This paper concludes that greater exchange rate flexibilityis in China’s own interest and that, along with a more stable and robust financial system, itshould be regarded as a prerequisite for undertaking a substantial liberalization of thecapital account.

Prediction of alloy composition and microhardness by random forest in maraging stainless steels based on a cluster formula

Fe-Ni-Cr-based super-high-strength maraging stainless steels were generally realized by multiple-element alloying under a given heat treatment processing. A series of alloy compositions were designed with a uniform cluster formula of [Ni16Fe192]（Cr32（Ni16-x-y-z-m-n MoxTiyNbzAlmVn）） （at.%） that was developed out of a unique alloy design tool, a cluster- plus-glue-atom model. Alloy rods with a diameter of 6 mm were prepared by copper-mold suction-cast processing under the argon atmosphere. These alloy samples were solid-solutioned at 1273 K for 1 h, followed by water-quenching, and then aged at 783 K for 3 h. The effect of the valence electron concentration, characterized with the number of valence electrons per unit cluster （VE/uc） formula of 16 atoms, on microhardness of these designed maraging stainless steels at both solid- solutioned and aged states was investigated. The relationship between alloy compositions and microhardness in maraging stainless steels was firstly established by the random forest （RF, a kind of machine learning methods） based on the experimental results. It was found that not only the microhardness of any given composition alloy within the frame of cluster formula, but also the alloy composition with a maximum microhardness for any given VE/uc, could be predicted in good agreement with the guidance of the relationship by RF. The contributions of minor-alloying elements to the microhardness of the aged alloys were also discussed.

基于同步伪随机数的无线通信安全方法

随着无线通信及其应用的飞速发展，其安全漏洞及脆弱性威胁也日益显现。虽然，IEEE 802.11无线局域网标准为人们提供了一个可选的、称之为WEP的数据认证和加密方案，但众所周知，WEP存在许多致命的不足，如数据截获侦听、IV(初始向量)的脆弱性、重播攻击以及只能实现单向认证等问题，致使无线网络具有许多脆弱性和威胁。

New Approach to WLAN Security with Synchronized Pseudo Random

Wireless transmission is becoming increasing ubiquitous, but there is a big black hole in the security of this kind of network. Although IEEE 802.11 provides an optional Wired Equivalent Privacy (WEP) to implement the authentication and confidentiality, it leaves a lot of vulnerabilities and threats. This paper proposes a protocol called SPRNG for wireless data-link layer security. SPRNG is based on the sender and receiver who generate in a synchronized way a pseudo-random number sequence. In each transmission, the sender and receiver use a pair of random numbers, one for data frame authentication, and the other for encryption key. The random numbers are used as 'one-time passwords' for sender authentication and as fresh encryption keys for each frame. SPRNG is designed to be compatible with the existing 802.11 products. Like WEP, the current 802.11 security protocol, SPRNG uses a symmetric key as its seed. SPRNG has already been simulated and tested in experiment, it shows that SPRNG has stronger Security than WEP because it reveals little information for attackers. The key problem of SPRNG, synchronization loss problem, is also presented. Though motivated by wireless security, SPRNG is generic for many other applications, especially in the point to point communication.

股市大跌影响的新评估

[摩根士丹利10月24日]我们在5月底曾发布《中国股市大跌，影响究竟有几何》一文，讨论股市泡沫破裂可能造成的影响。目前，上证指数已经比“530”之后的低点上涨了60％，9月份的静态市盈率已达64倍的历史高点。这种情况下，股市泡沫破裂将会造成的严重后果又一次引起了人们的担忧。投资者尤其担心实体经济有可能受到三方面的严重影响，即负面“财富效应”对居民消费的影响、企业固定资产投资的融资渠道受到的影响以及银行财务状况受到的影响。