A novel 2×5 model of insert-plug piezoelectric quartz crystal tumor marker micro-array immunosensor constructed with screw clamp apparatus has been developed for quantitative detection of the tumor markers such a...A novel 2×5 model of insert-plug piezoelectric quartz crystal tumor marker micro-array immunosensor constructed with screw clamp apparatus has been developed for quantitative detection of the tumor markers such as alpha-fetoprotein (AFP),carcino-embryonic antigen (CEA),prostate specific antigen (PSA),and human chorionic gonadotropin (hCG) in serum,in which every crystal unit can oscillate independently with the stability of±1 hertz (Hz) in air and±2 Hz in liquid.These response characteristics of Pz tumor marker micro-array immunosensor such as temperature, time-cost,reproducibility and specificity etc were also investigated.The detection ranges for AFP,CEA,PSA,and hCG obtained by Pz micro-array immunosensor were 20 ng/ml～640 ng/ml,1.56 ng/ml～50 ng/ml,1.25 ng/ml～50 ng/ml,and 2.5 mIU/ml～250 mlU/mi respectively with the coefficient of variance (CV) less than 5%.No cross-reactivates with other tumor markers in serum were observed.The results of AFP,CEA,PSA,and hCG obtained by this method from 68 serum samples were in good agreement with those given by chemiluminescence immunoassay with the correlation coefficients of 0.92,0.90,0.91,and 0.94 respectively.The Pz immunosensor regenerated by urea solution could be reused for five times without appreciable loss of response activity.Therefore,the proposed insert-plug immunosensor provides a rapid, sensitive,specific,reusable,convenient and reliable alternative for the detection of tumor markers in clinical laboratory.展开更多
Aiming at the rural-urban background, the paper research content is that the outdoor space design adjusting to aging population. It is noteworthy that the “empty nest” becomes a social phenomenon in China. The commu...Aiming at the rural-urban background, the paper research content is that the outdoor space design adjusting to aging population. It is noteworthy that the “empty nest” becomes a social phenomenon in China. The community and the village are the are population most centralized region, it is also the main places that old man consume their leisure time. Outdoor space design should be suitable for the local natural climatic conditions and cultural customs. Outdoor space in Hunan of China appropriates local opened space. The high quality design of outdoor space will create conditions for social endowment and self-help endowment. Outdoor activity space is closely linked with the old people’s quality of life. Function of outdoor space includes contact and the exercise as well as manual labor and pastime. Outdoor space design strategy consists of barrier-free design, identification design and activity space. The paper thinks that: “Doing to older, joy to older” is the highest state of old age. This needs the joint efforts of the family and community, needs to assume by physical space. The outdoor is the main site of activity for old man. Taking exercise, accompanying with others or pets, association and manual labor are the main content to outdoor space design.展开更多
文摘A novel 2×5 model of insert-plug piezoelectric quartz crystal tumor marker micro-array immunosensor constructed with screw clamp apparatus has been developed for quantitative detection of the tumor markers such as alpha-fetoprotein (AFP),carcino-embryonic antigen (CEA),prostate specific antigen (PSA),and human chorionic gonadotropin (hCG) in serum,in which every crystal unit can oscillate independently with the stability of±1 hertz (Hz) in air and±2 Hz in liquid.These response characteristics of Pz tumor marker micro-array immunosensor such as temperature, time-cost,reproducibility and specificity etc were also investigated.The detection ranges for AFP,CEA,PSA,and hCG obtained by Pz micro-array immunosensor were 20 ng/ml～640 ng/ml,1.56 ng/ml～50 ng/ml,1.25 ng/ml～50 ng/ml,and 2.5 mIU/ml～250 mlU/mi respectively with the coefficient of variance (CV) less than 5%.No cross-reactivates with other tumor markers in serum were observed.The results of AFP,CEA,PSA,and hCG obtained by this method from 68 serum samples were in good agreement with those given by chemiluminescence immunoassay with the correlation coefficients of 0.92,0.90,0.91,and 0.94 respectively.The Pz immunosensor regenerated by urea solution could be reused for five times without appreciable loss of response activity.Therefore,the proposed insert-plug immunosensor provides a rapid, sensitive,specific,reusable,convenient and reliable alternative for the detection of tumor markers in clinical laboratory.
文摘Aiming at the rural-urban background, the paper research content is that the outdoor space design adjusting to aging population. It is noteworthy that the “empty nest” becomes a social phenomenon in China. The community and the village are the are population most centralized region, it is also the main places that old man consume their leisure time. Outdoor space design should be suitable for the local natural climatic conditions and cultural customs. Outdoor space in Hunan of China appropriates local opened space. The high quality design of outdoor space will create conditions for social endowment and self-help endowment. Outdoor activity space is closely linked with the old people’s quality of life. Function of outdoor space includes contact and the exercise as well as manual labor and pastime. Outdoor space design strategy consists of barrier-free design, identification design and activity space. The paper thinks that: “Doing to older, joy to older” is the highest state of old age. This needs the joint efforts of the family and community, needs to assume by physical space. The outdoor is the main site of activity for old man. Taking exercise, accompanying with others or pets, association and manual labor are the main content to outdoor space design.
