Chronic long-term exposure to cuprizone causes severe brain demyelination in mice,which leads to changes in locomotion,working memory and anxiety.These findings suggest the importance of intact myelin for these behavi...Chronic long-term exposure to cuprizone causes severe brain demyelination in mice,which leads to changes in locomotion,working memory and anxiety.These findings suggest the importance of intact myelin for these behaviors.This study aimed to investigate the possible behavioral changes in mice with mild oligodendrocyte/myelin damage that parallels the white matter changes seen in the brains of patients with psychiatric disporders.We used the cuprizonetreated mouse model to test both tissue changes and behavioral functions（locomotor activity,anxiety status,and spatial working memory）.The results showed that mice given cuprizone in their diet for 7 days had no significant myelin breakdown as evaluated by immunohistochemical staining for myelin basic protein,while the number of mature oligodendrocytes was reduced.The number and length of Caspr protein clusters,a structural marker of the node of Ranvier,did not change.The locomotor activity of the cuprizonetreated mice increased whereas their anxiety levels were lower than in normal controls;spatial working memory,however,did not change.These results,for the first time,link emotion-related behavior with mild white matter damage in cuprizone-treated mice.展开更多
Immuno-suppressive protein, with a molecular weight of 155 and 370 ku, was generated in spleen and lymph node in stressed rats. It strongly inhibited lymphocyte proliferation. The results of activity assay, molecular ...Immuno-suppressive protein, with a molecular weight of 155 and 370 ku, was generated in spleen and lymph node in stressed rats. It strongly inhibited lymphocyte proliferation. The results of activity assay, molecular weight determination and histological localization all demonstrated that under stress the immuno-suppressive protein was also generated in intestinal mucosa of rats.展开更多
Immuno-suppressive protein was generated in spleen and lymph node and released into serum in mice and rats under stress, which had strong suppressive effects on T and B lymphocyte proliferation. The present study show...Immuno-suppressive protein was generated in spleen and lymph node and released into serum in mice and rats under stress, which had strong suppressive effects on T and B lymphocyte proliferation. The present study showed that the serum from stressed mice presented a significant decrease in lymphocyte proliferation at 1, 12, 24, and 48 h after stress. There was not significant suppressive effect at 96 h. The half life of biological effect was 21.6展开更多
基金supported by the Manitoba Health Research Council Foundationthe Canadian Institutes of Health Research Foundationthe Health Science Centre Foundation
文摘Chronic long-term exposure to cuprizone causes severe brain demyelination in mice,which leads to changes in locomotion,working memory and anxiety.These findings suggest the importance of intact myelin for these behaviors.This study aimed to investigate the possible behavioral changes in mice with mild oligodendrocyte/myelin damage that parallels the white matter changes seen in the brains of patients with psychiatric disporders.We used the cuprizonetreated mouse model to test both tissue changes and behavioral functions（locomotor activity,anxiety status,and spatial working memory）.The results showed that mice given cuprizone in their diet for 7 days had no significant myelin breakdown as evaluated by immunohistochemical staining for myelin basic protein,while the number of mature oligodendrocytes was reduced.The number and length of Caspr protein clusters,a structural marker of the node of Ranvier,did not change.The locomotor activity of the cuprizonetreated mice increased whereas their anxiety levels were lower than in normal controls;spatial working memory,however,did not change.These results,for the first time,link emotion-related behavior with mild white matter damage in cuprizone-treated mice.
文摘Immuno-suppressive protein, with a molecular weight of 155 and 370 ku, was generated in spleen and lymph node in stressed rats. It strongly inhibited lymphocyte proliferation. The results of activity assay, molecular weight determination and histological localization all demonstrated that under stress the immuno-suppressive protein was also generated in intestinal mucosa of rats.
文摘Immuno-suppressive protein was generated in spleen and lymph node and released into serum in mice and rats under stress, which had strong suppressive effects on T and B lymphocyte proliferation. The present study showed that the serum from stressed mice presented a significant decrease in lymphocyte proliferation at 1, 12, 24, and 48 h after stress. There was not significant suppressive effect at 96 h. The half life of biological effect was 21.6
