We report a complete genomic sequence of rare isolates (minor genotype) of theSARS-CoV from SARS patients in Guangdong, China, where the first few casesemerged. The most striking discovery from the isolate is an extra...We report a complete genomic sequence of rare isolates (minor genotype) of theSARS-CoV from SARS patients in Guangdong, China, where the first few casesemerged. The most striking discovery from the isolate is an extra 29-nucleotidesequence located at the nucleotide positions between 27,863 and 27,864 (referredto the complete sequence of B J01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream ofthe N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29,suggests a significant genetic event and differentiates it from the previously re-ported genotype, the dominant form among all sequenced SARS-CoV isolates. A17-nt segment of this extra sequence is identical to a segment of the same size intwo human mRNA sequences that may interfere with viral genome replication andtranscription in the cytosol of the infected cells. It provides a new avenue for theexploration of the virus-host interaction in viral evolution, host pathogenesis, andvaccine development.展开更多
The corona-like spikes or peplomers on the surface of the virion under electronicmicroscope are the most striking features of coronaviruses. The S (spike) proteinis the largest structural protein, with 1,255 amino aci...The corona-like spikes or peplomers on the surface of the virion under electronicmicroscope are the most striking features of coronaviruses. The S (spike) proteinis the largest structural protein, with 1,255 amino acids, in the viral genome. Itsstructure can be divided into three regions: a long N-terminal region in the exte-rior, a characteristic transmembrane (TM) region, and a short C-terminus in theinterior of a virion. We detected fifteen substitutions of nucleotides by comparisonswith the seventeen published SARS-CoV genome sequences, eight (53.3%) of whichare non-synonymous mutations leading to amino acid alternations with predictedphysiochemical changes. The possible antigenic determinants of the S protein arepredicted, and the result is confirmed by ELISA (enzyme-linked immunosorbentassay) with synthesized peptides. Another profound finding is that three disulfidebonds are defined at the C-terminus with the N-terminus of the E (envelope) pro-tein, based on the typical sequence and positions, thus establishing the structuralconnection with these two important structural proteins, if confirmed. Phyloge-netic analysis reveals several conserved regions that might be potent drug targets.展开更多
文摘We report a complete genomic sequence of rare isolates (minor genotype) of theSARS-CoV from SARS patients in Guangdong, China, where the first few casesemerged. The most striking discovery from the isolate is an extra 29-nucleotidesequence located at the nucleotide positions between 27,863 and 27,864 (referredto the complete sequence of B J01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream ofthe N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29,suggests a significant genetic event and differentiates it from the previously re-ported genotype, the dominant form among all sequenced SARS-CoV isolates. A17-nt segment of this extra sequence is identical to a segment of the same size intwo human mRNA sequences that may interfere with viral genome replication andtranscription in the cytosol of the infected cells. It provides a new avenue for theexploration of the virus-host interaction in viral evolution, host pathogenesis, andvaccine development.
文摘The corona-like spikes or peplomers on the surface of the virion under electronicmicroscope are the most striking features of coronaviruses. The S (spike) proteinis the largest structural protein, with 1,255 amino acids, in the viral genome. Itsstructure can be divided into three regions: a long N-terminal region in the exte-rior, a characteristic transmembrane (TM) region, and a short C-terminus in theinterior of a virion. We detected fifteen substitutions of nucleotides by comparisonswith the seventeen published SARS-CoV genome sequences, eight (53.3%) of whichare non-synonymous mutations leading to amino acid alternations with predictedphysiochemical changes. The possible antigenic determinants of the S protein arepredicted, and the result is confirmed by ELISA (enzyme-linked immunosorbentassay) with synthesized peptides. Another profound finding is that three disulfidebonds are defined at the C-terminus with the N-terminus of the E (envelope) pro-tein, based on the typical sequence and positions, thus establishing the structuralconnection with these two important structural proteins, if confirmed. Phyloge-netic analysis reveals several conserved regions that might be potent drug targets.
