Lens-free on-chip microscopy with RGB LEDs(LFOCM-RGB)provides a portable,cost-effective,and high-throughput imaging tool for resource-limited environments.However,the weak coherence of LEDs limits the high-resolution ...Lens-free on-chip microscopy with RGB LEDs(LFOCM-RGB)provides a portable,cost-effective,and high-throughput imaging tool for resource-limited environments.However,the weak coherence of LEDs limits the high-resolution imaging,and the luminous surfaces of the LED chips on the RGB LED do not overlap,making the coherence-enhanced executions tend to undermine the portable and cost-effective implementation.Here,we propose a specially designed pinhole array to enhance coherence in a portable and cost-effective implementation.It modulates the three-color beams from the RGB LED separately so that the three-color beams effectively overlap on the sample plane while reducing the effective light-emitting area for better spatial coherence.The separate modulation of the spatial coherence allows the temporal coherence to be modulated separately by single spectral filters rather than by expensive triple spectral filters.Based on the pinhole array,the LFOCM-RGB simply and effectively realizes the high-resolution imaging in a portable and cost-effective implementation,offering much flexibility for various applications in resource-limited environments.展开更多
A 60-days feeding trial was conducted to determine the effect of dietary supplementation of Lonicera macranthoides leaf(LML) on growth performance, meat quality, and free amino acid profiles in growingfinishing pigs. ...A 60-days feeding trial was conducted to determine the effect of dietary supplementation of Lonicera macranthoides leaf(LML) on growth performance, meat quality, and free amino acid profiles in growingfinishing pigs. L. macranthoides leaves were harvested, air-dried and milled to powder. A total of 288 crossbred barrows(Large White x Landrace) with an average initial BW of 54.0 ± 3.4 kg were randomly allocated to 3 treatment groups and fed either a basal diet or a diet supplemented with 0.5% or 1.0% LML powder. Results showed that diet supplemented with LML powder did not affect average daily feed intake(ADFI) and feed: gain ratio(P> 0.05) in growing-finishing pigs. Moreover, diet supplemented with LML powder showed no significant effects on carcass traits(P > 0.05) including dressing percentage,backfat thickness and loin muscle area, and meat quality traits(P > 0.05) including meat color, intramuscular fat and cooking loss. However, diet supplemented with LML powder significantly improved free amino acid profiles in serum and longissimus thoracis muscle in growing-finishing pigs. Most importantly, dietary LML powder increased total free amino acids content(P < 0.001) and essential free amino acid content(P < 0.001) in longissimus thoracis muscle. These results indicate that LML has the potential to improve the nutritional value of meat through improving free amino acid profiles.展开更多
This study was conducted to investigate the effects of catechins on reproductive performance, antioxidative capacity and immune function of gestating sows. A total of 60 cross-bred（Landrace × Large White） multi...This study was conducted to investigate the effects of catechins on reproductive performance, antioxidative capacity and immune function of gestating sows. A total of 60 cross-bred（Landrace × Large White） multiparious sows were blocked by body weight, parity and backfact and randomly allocated to 1 of 5 treatments: 0.100,200,300, or 400 mg/kg catechins. Dietary treatments were imposed from mating to d 40 of gestation of sows. At farrowing, litter total born, born alive, dead, and normal-（healthy piglets,>0.85 kg） and low-birth weight piglets（<0.85 kg） were recorded. Within 3.00 ± 0.50 days after farrowing litter size was standardized to 8,00 ± 1.50 piglets within treatment. The piglets were weighed at birth（d 1） and weaning（d 28）. Sows serum samples were obtained from blood samples collected on d 40 of gestation for analyses of glutathione peroxidase（GSH-Px）, superoxide dismutase（SOD）, catalase（CAT）,malondialdehyde（MDA）, hydrogen peroxide（H2 O2）, nitric oxide synthetase（NOS） and nitrogen monoxide（NO）. Our results showed that supplementation of catechins at levels of 200 or 300 mg/kg led to improvements in litter born alive（P < 0.01） and piglet born healthy（P < 0.01） and a decrease in stillborn（P < 0.05） at farrowing when compared with the control. In comparison with the control, catechins at any supplemental levels all enhanced the serum SOD（P < 0.05） and CAT（P < 0.01） activities of sows at farrowing but no obvious differences in the serum GSH-Px and NOS activities were observed in this trial（P > 0.05）. Sows received 200 mg catechin per kg diets showed a reduction（P < 0.05） of the serum MDA level at farrowing compared with all other treatments. Sows received all the levels of catechin showed a reduction（P < 0.05） of serum H2 O2 level compared with sows received the control diet on both d 40 of gestation and farrowing. Our results demonstrated that the catechins may be a potential antioxidant to increase the reproductive performance and antioxidative capacity of sows when it was added into diets during the early gestation.展开更多
基金supported by the Shenzhen Key Basic Program(No.JCYJ20200109143031287)the Shenzhen General Basic Program(No.WDZC20220816110140002)。
文摘Lens-free on-chip microscopy with RGB LEDs(LFOCM-RGB)provides a portable,cost-effective,and high-throughput imaging tool for resource-limited environments.However,the weak coherence of LEDs limits the high-resolution imaging,and the luminous surfaces of the LED chips on the RGB LED do not overlap,making the coherence-enhanced executions tend to undermine the portable and cost-effective implementation.Here,we propose a specially designed pinhole array to enhance coherence in a portable and cost-effective implementation.It modulates the three-color beams from the RGB LED separately so that the three-color beams effectively overlap on the sample plane while reducing the effective light-emitting area for better spatial coherence.The separate modulation of the spatial coherence allows the temporal coherence to be modulated separately by single spectral filters rather than by expensive triple spectral filters.Based on the pinhole array,the LFOCM-RGB simply and effectively realizes the high-resolution imaging in a portable and cost-effective implementation,offering much flexibility for various applications in resource-limited environments.
基金financial support from the Major Project of Hunan Province (2015NK1002)the National Science and Technology Support Program Funding (2012BAD39B03)Key Research and Development Program of Hunan Province(field of agricultural support) (2015NK2004, 2015JC3100)
文摘A 60-days feeding trial was conducted to determine the effect of dietary supplementation of Lonicera macranthoides leaf(LML) on growth performance, meat quality, and free amino acid profiles in growingfinishing pigs. L. macranthoides leaves were harvested, air-dried and milled to powder. A total of 288 crossbred barrows(Large White x Landrace) with an average initial BW of 54.0 ± 3.4 kg were randomly allocated to 3 treatment groups and fed either a basal diet or a diet supplemented with 0.5% or 1.0% LML powder. Results showed that diet supplemented with LML powder did not affect average daily feed intake(ADFI) and feed: gain ratio(P> 0.05) in growing-finishing pigs. Moreover, diet supplemented with LML powder showed no significant effects on carcass traits(P > 0.05) including dressing percentage,backfat thickness and loin muscle area, and meat quality traits(P > 0.05) including meat color, intramuscular fat and cooking loss. However, diet supplemented with LML powder significantly improved free amino acid profiles in serum and longissimus thoracis muscle in growing-finishing pigs. Most importantly, dietary LML powder increased total free amino acids content(P < 0.001) and essential free amino acid content(P < 0.001) in longissimus thoracis muscle. These results indicate that LML has the potential to improve the nutritional value of meat through improving free amino acid profiles.
基金jointly supported by grants from the National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2012BAD39B00)State Key Laboratory of Animal Nutrition(2004DA125184F1305)+2 种基金the NSFC(307005793110173031110103909)
文摘This study was conducted to investigate the effects of catechins on reproductive performance, antioxidative capacity and immune function of gestating sows. A total of 60 cross-bred（Landrace × Large White） multiparious sows were blocked by body weight, parity and backfact and randomly allocated to 1 of 5 treatments: 0.100,200,300, or 400 mg/kg catechins. Dietary treatments were imposed from mating to d 40 of gestation of sows. At farrowing, litter total born, born alive, dead, and normal-（healthy piglets,>0.85 kg） and low-birth weight piglets（<0.85 kg） were recorded. Within 3.00 ± 0.50 days after farrowing litter size was standardized to 8,00 ± 1.50 piglets within treatment. The piglets were weighed at birth（d 1） and weaning（d 28）. Sows serum samples were obtained from blood samples collected on d 40 of gestation for analyses of glutathione peroxidase（GSH-Px）, superoxide dismutase（SOD）, catalase（CAT）,malondialdehyde（MDA）, hydrogen peroxide（H2 O2）, nitric oxide synthetase（NOS） and nitrogen monoxide（NO）. Our results showed that supplementation of catechins at levels of 200 or 300 mg/kg led to improvements in litter born alive（P < 0.01） and piglet born healthy（P < 0.01） and a decrease in stillborn（P < 0.05） at farrowing when compared with the control. In comparison with the control, catechins at any supplemental levels all enhanced the serum SOD（P < 0.05） and CAT（P < 0.01） activities of sows at farrowing but no obvious differences in the serum GSH-Px and NOS activities were observed in this trial（P > 0.05）. Sows received 200 mg catechin per kg diets showed a reduction（P < 0.05） of the serum MDA level at farrowing compared with all other treatments. Sows received all the levels of catechin showed a reduction（P < 0.05） of serum H2 O2 level compared with sows received the control diet on both d 40 of gestation and farrowing. Our results demonstrated that the catechins may be a potential antioxidant to increase the reproductive performance and antioxidative capacity of sows when it was added into diets during the early gestation.
