An enolase-encoding cDNA clone in oilseed rape (Brassica napus L.) was isolated. This gene (accession number: AY307449) had a total length of 1 624 bp with an open reading frame of 1 335 bp, and encoded a predicted po...An enolase-encoding cDNA clone in oilseed rape (Brassica napus L.) was isolated. This gene (accession number: AY307449) had a total length of 1 624 bp with an open reading frame of 1 335 bp, and encoded a predicted polypeptide of 444 amino acids with a molecular weight of 47.38 kD. The deduced amino acid sequence shared identity with a number of enolases ranging from Bacillus subtilis to human beings and had much higher identity with other plant enolases than with enolases from Bacillus, yeast and human beings. Comparison of its primary structure with those of other enolases revealed the presence of an insertion of five amino acids in enolase of B. napus. Southern blotting analysis of genomic DNA indicated that enolase was likely to be a low-copy gene in the oilseed rape genome. Expression of the cloned enolase gene increased under salt stress, but decreased in response to low temperature. Our studies suggested that the cloned gene was a new member of plant enolase gene family, which contributed to the energy supply in stress-treated tissues.展开更多
There has been quite a lot of discussions centered on postmenopau sal hormone replacement therapy(HRT), but far less on the perimenopausal transit ion. Research has been done on the aging ovary and showed alterati... There has been quite a lot of discussions centered on postmenopau sal hormone replacement therapy(HRT), but far less on the perimenopausal transit ion. Research has been done on the aging ovary and showed alterations in the pri mary unit, the follicle; and granulosa cell is the most important factor respons ible for this follicle change. The aging ovary contains fewer follicles and fewer granulosa cells produced less amount of steroids and many other important factors to the survival of the ovum causing increased rate of granulosa cell apo ptosis in each follicle. Many of these changes may start before the menopause.……展开更多
文摘An enolase-encoding cDNA clone in oilseed rape (Brassica napus L.) was isolated. This gene (accession number: AY307449) had a total length of 1 624 bp with an open reading frame of 1 335 bp, and encoded a predicted polypeptide of 444 amino acids with a molecular weight of 47.38 kD. The deduced amino acid sequence shared identity with a number of enolases ranging from Bacillus subtilis to human beings and had much higher identity with other plant enolases than with enolases from Bacillus, yeast and human beings. Comparison of its primary structure with those of other enolases revealed the presence of an insertion of five amino acids in enolase of B. napus. Southern blotting analysis of genomic DNA indicated that enolase was likely to be a low-copy gene in the oilseed rape genome. Expression of the cloned enolase gene increased under salt stress, but decreased in response to low temperature. Our studies suggested that the cloned gene was a new member of plant enolase gene family, which contributed to the energy supply in stress-treated tissues.
文摘 There has been quite a lot of discussions centered on postmenopau sal hormone replacement therapy(HRT), but far less on the perimenopausal transit ion. Research has been done on the aging ovary and showed alterations in the pri mary unit, the follicle; and granulosa cell is the most important factor respons ible for this follicle change. The aging ovary contains fewer follicles and fewer granulosa cells produced less amount of steroids and many other important factors to the survival of the ovum causing increased rate of granulosa cell apo ptosis in each follicle. Many of these changes may start before the menopause.……
