Hederacolchiside A1 suppresses proliferation of tumor cells by inducing apoptosis through modulating PI3K/Akt/mTOR signaling pathway

Objective：Hederacolchiside A1,exhibits cytostatic and cytotoxic activity against various cancer cells in vitro,however,the mechanism is not well understood.Methods：In this study,hederacolchiside A1 from Pulsatilla chinensis was isolated and tested its anticancer activity and mechanism.Hederacolchiside A1 could inhibit proliferation of A549,SMMC-7721,BEL-7402,and MCF-7 cells by MTT assay.Investigations of apoptosis of treated cancer cells were identified in hederacolchiside A1 by flow cytometric analysis of annexin V expression.Results：Based on the results of western blotting and JC-1 staining,hederacolchiside A1 reduced the mitochondrial membrane potential and Bcl-2 protein levels,whereas cleaved caspase-3 was higher.Furthermore,hederacolchiside A1 effectively inhibited the phosphorylations of phosphatidylinositol 3 kinase（PI3K）,protein kinase B（Akt）,and mammalian target of rapamycin（m TOR）.In vivo study showed that hederacolchiside A1（3.0,4.5,and 6.0 mg/kg,ip）could significantly inhibit the weight of tumor in anmodel.Similar inhibitory activities were observed when the compound（3.25,7.5,and15.0 mg/kg,ig）was tested in nude mice xenograft tumor models using human breast carcinoma MCF-7 cells.Conclusion：These data indicated that hederacolchiside A1 suppressed the proliferation of human tumor cells by inducing apoptosis through modulating the PI3K/Akt/m TOR signaling pathway.

A New Triterpenoid Saponin from Aidi Injection

Objective To investigate the chemical constituents from Aidi Injection.Methods The chemical constituents were isolated by chromatography on Sephadex LH-20 gel columns and reverse phase semi-preparative HPLC repeatedly.Their structures were identified by spectroscopic analysis(NMR and MS).Results Twenty-two compounds were isolated and identified to be 3-O-3′,4′-diacetyl-β-D-xylopyranosyl-6-O-β-D-glucopyranosyl- cycloastragenol(1),astragaloside IV(2),astragaloside II(3),astragaloside I(4),isoastragaloside I(5), acetylastragaloside I(6),ginsenosid Re(7),ginsenoside Rf(8),ginsenoside Rg1(9),ginsenoside Rb3(10), notoginsenoside R4(11),ginsenoside Rb1(12),ginsenoside Rc(13),ginsenoside Rb2(14),ginsenoside Rd(15), lucyoside H(16),3-O-β-D-glucopyranosyl(1→4)-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl(1→2)-α-L- arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside (17),3-O-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl[β-D-glucopyranosyl-(1→4)]-(1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-arabinopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside(18),syringin (19),elentheroside E(20),4-(1,2,3-trihydroxypropyl)-2,6-dimethoxyphenyl-1-O-β-D-glucopyranoside(21),and coniferin(22).Conclusion Compounds 1-6 are originated from Astragalus membranceus,compounds 7-18 are originated from Panax ginseng,and compounds 19-22 are originated from Acanthopanax senticosus by LC-MS analysis.Compound 1 is a new compound.