期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
一种简单经济提取小鼠大量肾小球的实验方法
1
作者 李艳秋 刘晓丹 +4 位作者 范秋灵 田淑艳 刘雪 王力宁 姚丽 《中国现代医学杂志》 CAS 2020年第7期10-13,共4页
目的为减轻实验者用磁珠提取肾小球的经济负担,对传统的心脏灌注肾脏提取方法进行改良。方法结扎远端腹主动脉及下腔静脉,结扎肠系膜上动脉和腹腔动脉,以减少磁珠分流。将磁珠工作液经静脉留置针灌注肾脏。加入相应体积胶原酶A,通过100... 目的为减轻实验者用磁珠提取肾小球的经济负担,对传统的心脏灌注肾脏提取方法进行改良。方法结扎远端腹主动脉及下腔静脉,结扎肠系膜上动脉和腹腔动脉,以减少磁珠分流。将磁珠工作液经静脉留置针灌注肾脏。加入相应体积胶原酶A,通过100μm筛网2次,加入蛋白裂解液,离心裂解吸出含蛋白上清进行肾小球蛋白的纯化、定量。结果与传统的心脏灌注肾脏提取方法比较,改良胸主动脉灌注肾脏提取肾小球方法磁珠用量仅为原来的1/35~1/40。在获取相同的蛋白情况下,提取肾小球的最佳浓度为4×10~6磁珠/ml,磁珠用量为20ml。通过该方法灌注分离的肾小球完整,纯度为(95.7±2.3)%。结论改良的胸主动脉灌注肾脏是一种简单经济实用磁珠提取肾小球的实验方法,可以快速获取大量完整的肾小球,蛋白质甚至RNA,为肾小球本身的研究提供新的技术支持。 展开更多
关键词 改良 肾小球 C57BL/6小鼠 NZB/WF1小鼠 磁珠
下载PDF
Machine learning in nephrology: scratching the surface 被引量:6
2
作者 Qi Li qiu-ling fan +5 位作者 Qiu-Xia Han Wen-Jia Geng Huan-Huan Zhao Xiao-Nan Ding Jing-Yao Yan Han-Yu Zhu 《Chinese Medical Journal》 SCIE CAS CSCD 2020年第6期687-698,共12页
Machine learning shows enormous potential in facilitating decision-making regarding kidney diseases.With the development of data preservation and processing,as well as the advancement of machine learning algorithms,ma... Machine learning shows enormous potential in facilitating decision-making regarding kidney diseases.With the development of data preservation and processing,as well as the advancement of machine learning algorithms,machine learning is expected to make remarkable breakthroughs in nephrology.Machine learning models have yielded many preliminaries to moderate and several excellent achievements in the fields,including analysis of renal pathological images,diagnosis and prognosis of chronic kidney diseases and acute kidney injury,as well as management of dialysis treatments.However,it is just scratching the surface of the field;at the same time,machine learning andits applications in renal diseases are facing a number of challenges.In this review,we discuss the application status,challenges and future prospects of machine learning in nephrology to help people further understand and improve the capacity for prediction,detection,and care quality in kidney diseases. 展开更多
关键词 MACHINE learning NEPHROLOGY KIDNEY DISEASES
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部