Due to our negligence,the original version of this article,published online on 12 January 2022,contained a mistake in Fig.4A.The lane ofβ-actin in Western blotting was misused.The correct Fig.4 is given below.We apol...Due to our negligence,the original version of this article,published online on 12 January 2022,contained a mistake in Fig.4A.The lane ofβ-actin in Western blotting was misused.The correct Fig.4 is given below.We apologize for our oversight when preparing the figure and state that this does not change the scientific conclusions of the article in any way.展开更多
Dear Editor,Rabbit hemorrhagic disease(RHD)is a fatal infectious disease that primarily affects adult rabbits and causes great economic losses in the rabbit industry(Mitro and Krauss,1993).The causative pathogen is ra...Dear Editor,Rabbit hemorrhagic disease(RHD)is a fatal infectious disease that primarily affects adult rabbits and causes great economic losses in the rabbit industry(Mitro and Krauss,1993).The causative pathogen is rabbit hemorrhagic disease virus(RHDV),which belongs to the genus Lagovirus and the family Calicivirus(Moussa et al.,1992).In 1984,RHDV was first documented in China.展开更多
Rabbit hemorrhagic disease virus(RHDV)is a member of the Caliciviridae family and cannot be propagated in vitro,which has impeded the progress of investigating its replication mechanism.Construction of an RHDV replico...Rabbit hemorrhagic disease virus(RHDV)is a member of the Caliciviridae family and cannot be propagated in vitro,which has impeded the progress of investigating its replication mechanism.Construction of an RHDV replicon system has recently provided a platform for exploring RHDV replication in host cells.Here,aided by this replicon system and using two-step affinity purification,we purified the RHDV replicase and identified its associated host factors.We identified rabbit nucleolin(NCL)as a physical link,which mediating the interaction between other RNA-dependent RNA polymerase(Rd Rp)-related host proteins and the viral replicase Rd Rp.We found that the overexpression or knockdown of NCL significantly increased or severely impaired RHDV replication in RK-13 cells,respectively.NCL was identified to directly interact with RHDV Rd Rp,p16,and p23.Furthermore,NCL knockdown severely impaired the binding of Rd Rp to Rd Rp-related host factors.Collectively,these results indicate that the host protein NCL is essential for RHDV replication and acts as a physical link between viral replicase and host proteins.展开更多
文摘Due to our negligence,the original version of this article,published online on 12 January 2022,contained a mistake in Fig.4A.The lane ofβ-actin in Western blotting was misused.The correct Fig.4 is given below.We apologize for our oversight when preparing the figure and state that this does not change the scientific conclusions of the article in any way.
基金the Chinese Natural Sciences Foundation(No.32172832,No.32000109)Shanghai Sailing Program(20YF1457700)the China Postdoctoral Science Foundation(No.2019M660885,No.2021T140718)。
文摘Dear Editor,Rabbit hemorrhagic disease(RHD)is a fatal infectious disease that primarily affects adult rabbits and causes great economic losses in the rabbit industry(Mitro and Krauss,1993).The causative pathogen is rabbit hemorrhagic disease virus(RHDV),which belongs to the genus Lagovirus and the family Calicivirus(Moussa et al.,1992).In 1984,RHDV was first documented in China.
基金sponsored by Shanghai Sailing Program(20YF1457700)the National Natural Science Foundation of China(32000109 and 31672572)the China Postdoctoral Science Foundation(2019M660885 and 2021T140718)
文摘Rabbit hemorrhagic disease virus(RHDV)is a member of the Caliciviridae family and cannot be propagated in vitro,which has impeded the progress of investigating its replication mechanism.Construction of an RHDV replicon system has recently provided a platform for exploring RHDV replication in host cells.Here,aided by this replicon system and using two-step affinity purification,we purified the RHDV replicase and identified its associated host factors.We identified rabbit nucleolin(NCL)as a physical link,which mediating the interaction between other RNA-dependent RNA polymerase(Rd Rp)-related host proteins and the viral replicase Rd Rp.We found that the overexpression or knockdown of NCL significantly increased or severely impaired RHDV replication in RK-13 cells,respectively.NCL was identified to directly interact with RHDV Rd Rp,p16,and p23.Furthermore,NCL knockdown severely impaired the binding of Rd Rp to Rd Rp-related host factors.Collectively,these results indicate that the host protein NCL is essential for RHDV replication and acts as a physical link between viral replicase and host proteins.
