PARK1 gene mutation of autosomal dominant Parkinson's disease family

Studies have shown that PARK1 gene is associated with the autosomal dominant inheritance of Parkinson＇s disease. PARK1 gene contains two mutation sites, namely Ala30Pro and Ala53Thr, which are located on exons 3 and 4, respectively. However, the genetic loci of the pathogenic genes remain unclear. In this study, blood samples were collected from 11 members of a family with high prevalence of Parkinson＇s disease, including four affected cases, five suspected cases and two non-affected cases. Point mutation screening of common mutation sites on PARK1 gene exon 4 was conducted using PCR, to determine the genetic loci of the causative gene for Parkinson＇s disease. Gene identification and sequencing results showed that a T base deletion mutation was observed in the PARK1 gene exon 4 of all 11 collected samples. It was confirmed that the PARK1 gene exon 4 gene mutation is an important pathogenic mutation for Parkinson＇s disease.

Neuronal apoptosis and interleukin 1-beta converting enzyme expression in the frontal cortex and hippocampus of mice with ischemia/reperfusion injury

BACKGROUND： Interleukin 1β-converting enzyme （ICE） gene expression can induce neuronal apoptosis. However, the dynamic changes in ICE gene expression and its effects on neuronal apoptosis under cerebral ischemia/reperfusion conditions remain unclear. OBJECTIVE： To observe neuronal apoptosis and changes in ICE gene expression in the frontal cortex and hippocampus following ischemia/reperfusion injury. DESIGN, TIME AND SETTING： A randomized, controlled animal study was conducted at the Laboratory of Experimental Animal Center, the Second Hospital of Jilin University and Central Laboratory, the Second Hospital of Jilin University, China, from November 2008 to September 2009. MATERIALS： The ICE gene primer sequence （TaKaPa Co., Dalian, China）, FACScan Flow cytometer （Becton Dickinson, Franklin Lakes, N J, USA）, and Perkin Elmer GeneAmp PCR system 2400 （Perkin Elmer, Waltham, MA, USA） were used in this study. METHODS： A total of 45 healthy, adult, male, Kunming mice were randomly assigned to normal control （n = 5）, sham surgery （n = 5）, and model （n = 35） groups. The mice in the model group were equally and randomly subdivided into seven subgroups according to various reperfusion time points （1 hour, 1,3, 7, 14, 28, and 42 days）. Animal models of ischemia/reperfusion injury were established by bilateral carotid artery ligation in the model group. The mice in the sham surgery group only received saline perfusion and surgery for carotid artery exposure. MAIN OUTCOME MEASURES： Neuronal apoptosis in the frontal cortex and hippocampus of mice was measured using flow cytometry. The time course of ICE mRNA levels in the frontal cortex and hippocampus were quantified using reverse transcription-polymerase chain reaction. RESULTS： Neuronal apoptosis in the frontal cortex and hippocampus peaked at 3 days following ischemia/reperfusion injury （P 〈 0.05）. ICE mRNA expression increased in the frontal cortex at 1 day following ischemia/reperfusion injury （P 〈 0.05）, decreased at 3 days, and then peaked at 14 days （P 〈 0.05）. ICE mRNA expression increased in the hippocampus at 3 days following ischemia/reperfusion injury （P 〈 0.05）, peaked at 7 days （P 〈 0.05）, and then decreased gradually to normal levels at 28 days. CONCLUSION： Neuronal apoptosis peaked at 3 days following ischemia/reperfusion injury, and both apoptosis and ICE mRNA levels remained high for 2 weeks after injury. Early apoptosis may result from increased ICE mRNA expression.

A survey of public needs for government information service in libraries

Purpose:This paper describes a comprehensive user survey carried out in China to investigate public needs for government information service in libraries.It is expected to provide insights into the ways to help public libraries meet the user needs.Design/methodology/approach:The paper is founded on a survey among library users,librarians and non-library users.Paper questionnaires were distributed in the National Library of China and 32 provincial public libraries in China and electronic questionnaires were sent to non-library users.A total of 909 valid questionnaires were collected.Findings:A lot of attention is paid to policies,laws and regulations relating to common people’s work and lives such as science,technology,education,culture,finance,market supervision,health care,sports,urban construction and environment protection.A wide variety of government information resources is needed and information delivered to users shall be accurate and timely.Internet has become the main means of accessing government information and mobile phones the preferred Web access channel,while printed materials are considered important information sources.Users need assistance and training in the use of library information resources and services.Research limitations:The sample was limited to respondents from provincial-level public libraries,but city and county-level library users were not included.Practical implications:The survey results are beneficial for the Chinese public libraries to design service programs and improve their services.Originality/value:The paper is one of the comprehensive surveys on public needs for government information service in the Chinese libraries and some of die findings will be of wider interest for other libraries.

全球视野下的稳增长——第二次世界大战以来的增长目标与政府支出

本文从政府支出切入,考察全球视野下的稳增长。本文在理论上贡献识别稳增长力度的方法,在实证上利用手工收集的全球增长目标数据进行检验。本文发现,当需要稳增长时,增长目标每增加1个百分点,政府支出平均增加1.148个百分点。这个发现是稳健的。本文发现稳增长的三个特征:在支出结构上,稳增长增加经济事务支出;在资金来源上,稳增长依靠政府经常性收入;在时间维度上,稳增长具有暂时性。本文的发现表明稳增长是全球性现象。

有机种植对农田节肢动物多样性影响的整合分析

有机农业是生态环境友好的生产方式,对农业生物多样性保护具有重要意义。个体研究的差异不利于有机农业的生态环境效益评估。本研究利用文献整合分析,以对农田生态环境具有良好指示作用的节肢动物为研究对象,探讨了有机种植对农田生物多样性的保护效果及影响因素。结果表明,相比常规种植,有机种植可使节肢动物的丰富度、多度及均匀度显著提升34.95%、64.95%及12.09%;天敌和害虫的物种丰富度分别显著提升了22.50%和31.03%;同时天敌的个体数量比常规显著增加了71.80%,害虫减少了10.46%。经过3年及以上的有机种植后,节肢动物的丰富度和多度均显著高于常规种植。常规种植化学农药施用频率可显著影响节肢动物丰富度和均匀度指数,施药次数每增加1次,节肢动物丰富度相比有机种植显著降低13.54%,均匀度显著降低2.64%。有机水田对节肢动物多度的提升效果显著优于有机旱地,为后者的4.7倍;但二者的丰富度和均匀度没有显著差异。蔬菜和茶叶种植体系对节肢动物多样性指数的综合提升效果优于其他作物类型,丰富度、多度和均匀度指数分别显著提升了81.46%、74.14%、18.55%和48.86%、49.06%、30.88%。鼓励常规种植减少农药施用频次,加大有机和生态化管理措施的应用程度,是保护农业生物多样性、实现农业绿色高质量发展的有效途径。

A Constructive Proof of Beurling-Lax Theorem

This paper deals with an alternative proof of Beurling-Lax theorem by adopting a constructive approach instead of the isomorphism technique which was used in the original proof.

Spectra of rational orthonormal systems

We use the Weyl correspondence approach to investigate the spectral operators of the Laguerre and the Kautz systems. We show that the basic functions in the Laguerre and the Kautz systems are the eigenvectors of modified Sturm-Liouville operators. The results are further generalized to multiple parameters of one complex variable in both the unit disc and the upper half-plane contexts.