[Objectives] To determine the optimum conditions and antioxidant activity of Myrica ruba leaf pigment,and provide reference for further development and use of Myrica ruba leaves. [Methods]Pigment of Myrica ruba leaves...[Objectives] To determine the optimum conditions and antioxidant activity of Myrica ruba leaf pigment,and provide reference for further development and use of Myrica ruba leaves. [Methods]Pigment of Myrica ruba leaves was extracted by ethanol extraction method. Absorbance was measured at wavelength of 300 nm. Effects of ethanol volume fraction,solid-to-liquid ratio,temperature,and extraction time were studied. L_9( 3~3) orthogonal experiment was carried out to optimize the extraction process,and DPPH scavenging ability was measured.[Results]The optimum extraction process of Myrica ruba leaf pigment was as follows: the ethanol volume fraction was 50%; the solid-to-liquid ratio was 1: 60( w/v),extraction temperature 70℃; extraction time was 60 min; after the concentration of purified Myrica ruba leaf pigment reached 0. 4455 mg/mL,the DPPH scavenging ability was basically the same as the same concentration Vc.[Conclusions] The experiment indicates that the optimized extraction process has a high yield rate,and Myrica ruba leaf pigment has high anti-oxidant activity.展开更多
[Objectives] To compare different extraction methods for total alkaloids from Abrus Cantoniensis Hance taking chloroform as solvent. [Methods] The ethanol extraction method,ultrasonic extraction method,acid water extr...[Objectives] To compare different extraction methods for total alkaloids from Abrus Cantoniensis Hance taking chloroform as solvent. [Methods] The ethanol extraction method,ultrasonic extraction method,acid water extraction method,and Soxhlet extraction method were used to extract total alkaloids,and the total alkaloids of Abrus Cantoniensis Hance were measured at 238 nm wavelength. [Results] The extraction rate of ethanol extraction method was 14. 76 mg/100 g; the extraction rate of ultrasonic extraction method was 56. 85 mg/100 g; the extraction rate of acid water extraction method was 56. 63 mg/100 g; the extraction rate of Soxhlet extraction method was 58. 19 mg/100 g.[Conclusions]Results indicate that Soxhlet extraction method can obtain the highest extraction rate.展开更多
基金Supported by Student’s Platform for Innovation and Entrepreneurship Training Program of Guangxi Zhuang Autonomous Region,China in 2016(201610599033)Innovation and Entrepreneurship Ethnical Medicine Teaching Team Program of Guangxi Zhuang Autonomous Region,China(Gui Jiao Gao Jiao[2015]93&Gui Jiao Gao Jiao[2016]6)
文摘[Objectives] To determine the optimum conditions and antioxidant activity of Myrica ruba leaf pigment,and provide reference for further development and use of Myrica ruba leaves. [Methods]Pigment of Myrica ruba leaves was extracted by ethanol extraction method. Absorbance was measured at wavelength of 300 nm. Effects of ethanol volume fraction,solid-to-liquid ratio,temperature,and extraction time were studied. L_9( 3~3) orthogonal experiment was carried out to optimize the extraction process,and DPPH scavenging ability was measured.[Results]The optimum extraction process of Myrica ruba leaf pigment was as follows: the ethanol volume fraction was 50%; the solid-to-liquid ratio was 1: 60( w/v),extraction temperature 70℃; extraction time was 60 min; after the concentration of purified Myrica ruba leaf pigment reached 0. 4455 mg/mL,the DPPH scavenging ability was basically the same as the same concentration Vc.[Conclusions] The experiment indicates that the optimized extraction process has a high yield rate,and Myrica ruba leaf pigment has high anti-oxidant activity.
基金Supported by the 12th Five-Year TCM Key Discipline Chinese Medicine Chemistry Construction Program of State Administration of Traditional Chinese Medicine,China(Guo Zhong Yi Yao Ren Jiao Fa[2012]32)Natural Science Foundation Project of Guangxi(2013GXNSFAA019240)+2 种基金Key Discipline Chinese Medicine Chemistry Construction Program of Guangxi,China(Gui Jiao Ke Yan[2013]16)Student’s Platform for Innovation and Entrepreneurship Training Program of Guangxi in 2015(201510599037)Innovation and Entrepreneurship Ethnical Medicine Teaching Team Program of Guangxi Zhuang Autonomous Region,China(Gui Jiao Gao Jiao[2015]93 & Gui Jiao Gao Jiao[2016]6)
文摘[Objectives] To compare different extraction methods for total alkaloids from Abrus Cantoniensis Hance taking chloroform as solvent. [Methods] The ethanol extraction method,ultrasonic extraction method,acid water extraction method,and Soxhlet extraction method were used to extract total alkaloids,and the total alkaloids of Abrus Cantoniensis Hance were measured at 238 nm wavelength. [Results] The extraction rate of ethanol extraction method was 14. 76 mg/100 g; the extraction rate of ultrasonic extraction method was 56. 85 mg/100 g; the extraction rate of acid water extraction method was 56. 63 mg/100 g; the extraction rate of Soxhlet extraction method was 58. 19 mg/100 g.[Conclusions]Results indicate that Soxhlet extraction method can obtain the highest extraction rate.
