Microbial synthesis of carotenoids is a highly desirable alternative to plant extraction and chemical synthesis.In this study,we investigated multidimensional strategies to improve the carotenoid synthesis in the indu...Microbial synthesis of carotenoids is a highly desirable alternative to plant extraction and chemical synthesis.In this study,we investigated multidimensional strategies to improve the carotenoid synthesis in the industrial workhorse of Saccharomyces cerevisiae.First,we rewired the yeast central metabolism by optimizing non-oxidative glycolysis pathway for an improved acetyl-CoA supply.Second,we restricted the consumption of farnesyl pyrophosphate(FPP)by the down-regulation of squalene synthase using the PEST degron.Third,we further explored the human lipid binding/transfer protein saposin B(hSapB)-mediated metabolic sink for an enhanced storage of lipophilic carotenoids.Last,the copper-induced GAL expression system was engineered to function in the yeast-peptone-dextrose medium for an increased biomass accumulation.By combining the abovementioned strategies,the final engineered yeast produced 166.79±10.43 mg/Ⅰβ-carotene in shake flasks,which was nearly 5-fold improvement of the parental carotenoid-producing strain.Together,we envision that multidimensional strategies reported here might be applicable to other hosts for the future industrial development of carotenoid synthesis from renewable feedstocks.展开更多
基金support from the National Natural Science Foundation of China(32270087)the Natural Science Foundation of Fujian Province of China(2020J05011)+2 种基金Guangdong Basic and Applied Basic Research Foundation(2021A1515110340)Xiamen University(0660X2510200)Daan Gene(20223160A0063),and ZhenSheng Biotech.
文摘Microbial synthesis of carotenoids is a highly desirable alternative to plant extraction and chemical synthesis.In this study,we investigated multidimensional strategies to improve the carotenoid synthesis in the industrial workhorse of Saccharomyces cerevisiae.First,we rewired the yeast central metabolism by optimizing non-oxidative glycolysis pathway for an improved acetyl-CoA supply.Second,we restricted the consumption of farnesyl pyrophosphate(FPP)by the down-regulation of squalene synthase using the PEST degron.Third,we further explored the human lipid binding/transfer protein saposin B(hSapB)-mediated metabolic sink for an enhanced storage of lipophilic carotenoids.Last,the copper-induced GAL expression system was engineered to function in the yeast-peptone-dextrose medium for an increased biomass accumulation.By combining the abovementioned strategies,the final engineered yeast produced 166.79±10.43 mg/Ⅰβ-carotene in shake flasks,which was nearly 5-fold improvement of the parental carotenoid-producing strain.Together,we envision that multidimensional strategies reported here might be applicable to other hosts for the future industrial development of carotenoid synthesis from renewable feedstocks.
