In order to reduce the use of peat resources and realize the sustainable development of tobacco, the pH of distiller s grain substrate was adjusted with humic acid, and the effects of various distiller s grain substra...In order to reduce the use of peat resources and realize the sustainable development of tobacco, the pH of distiller s grain substrate was adjusted with humic acid, and the effects of various distiller s grain substrates on the growth of tobacco seedlings were studied to find out the method of cultivating high-quality tobacco seedlings by using distiller s grain substrate. The results showed that when 60% of humic acid was added to the distiller s grain substrate made from coarse distiller s grains, fine distiller s grains, perlite and vermiculite, both the pH and electrical conductivity decreased significantly and were similar to that of the conventional substrate. Moreover, the emergence rate of tobacco seedlings, the rate of strong tobacco seedlings, leaf number, stem height, root growth and quality of tobacco seedlings were good.展开更多
本研究目的是探讨血清N-聚糖模型在285例丙氨酸转移酶(alanine aminotransferase,ALT)水平正常(<40 U·L^(-1))的慢性乙型肝炎(慢性乙肝)患者中诊断显著肝纤维化和肝硬化的临床意义。入组患者均进行肝组织活检,并使用Ishak评分...本研究目的是探讨血清N-聚糖模型在285例丙氨酸转移酶(alanine aminotransferase,ALT)水平正常(<40 U·L^(-1))的慢性乙型肝炎(慢性乙肝)患者中诊断显著肝纤维化和肝硬化的临床意义。入组患者均进行肝组织活检,并使用Ishak评分系统评估患者肝组织纤维化程度。应用基于DNA测序仪的荧光糖电泳技术检测患者血清N-聚糖图谱,每例患者的血清样本中共鉴定出9个N-聚糖峰。利用机器学习算法,即随机森林(random forest,RF)构建更理想的血清N-聚糖模型,以诊断显著肝纤维化(≥F3)和肝硬化(≥F5),并比较血清N-聚糖模型和其他纤维化标志物的诊断效能。肝组织活检结果显示,有显著肝纤维化和肝硬化患者分别占63.86%(182/285)和16.49%(47/285),有显著炎症患者为4.91%(14/285)。血清N-聚糖RF-A模型具有很好的诊断显著肝纤维化(≥F3)的效能,其受试者工作特征曲线下面积(area under receiver operating characteristic curve,AUROC)为0.94,与肝活检的符合率为90.45%。在诊断肝硬化(≥F5)时,血清N-聚糖RF-B模型的AUROC为0.97,与肝组织活检的符合率为88.94%。血清N-聚糖模型(RF-A和RF-B)的诊断效能优于肝硬度值测量(liver stiffness measurement,LSM)、基于4因子的纤维化指数(fibrosis index based on the four factors,FIB-4)和天冬氨酸转氨酶与血小板比率指数(aspartate aminotransferase-to-platelet ratio index,APRI)。在ALT水平正常的慢性乙肝患者中,血清N-聚糖模型可作为诊断显著肝纤维化或肝硬化的潜在生物标志物。展开更多
基金Supported by the Project of Luzhou Company of Sichuan Provincial Tobacco Company"Comprehensive Application of Distillers'Grains on Tobacco"
文摘In order to reduce the use of peat resources and realize the sustainable development of tobacco, the pH of distiller s grain substrate was adjusted with humic acid, and the effects of various distiller s grain substrates on the growth of tobacco seedlings were studied to find out the method of cultivating high-quality tobacco seedlings by using distiller s grain substrate. The results showed that when 60% of humic acid was added to the distiller s grain substrate made from coarse distiller s grains, fine distiller s grains, perlite and vermiculite, both the pH and electrical conductivity decreased significantly and were similar to that of the conventional substrate. Moreover, the emergence rate of tobacco seedlings, the rate of strong tobacco seedlings, leaf number, stem height, root growth and quality of tobacco seedlings were good.
基金supported by the Major Science and Technology Special Project of China Thirteenth Five-year Plan(2018ZX10732401-003-015)the National Science and Technology Major Project(2013ZX10002005 and 2017ZX10203202)。
文摘本研究目的是探讨血清N-聚糖模型在285例丙氨酸转移酶(alanine aminotransferase,ALT)水平正常(<40 U·L^(-1))的慢性乙型肝炎(慢性乙肝)患者中诊断显著肝纤维化和肝硬化的临床意义。入组患者均进行肝组织活检,并使用Ishak评分系统评估患者肝组织纤维化程度。应用基于DNA测序仪的荧光糖电泳技术检测患者血清N-聚糖图谱,每例患者的血清样本中共鉴定出9个N-聚糖峰。利用机器学习算法,即随机森林(random forest,RF)构建更理想的血清N-聚糖模型,以诊断显著肝纤维化(≥F3)和肝硬化(≥F5),并比较血清N-聚糖模型和其他纤维化标志物的诊断效能。肝组织活检结果显示,有显著肝纤维化和肝硬化患者分别占63.86%(182/285)和16.49%(47/285),有显著炎症患者为4.91%(14/285)。血清N-聚糖RF-A模型具有很好的诊断显著肝纤维化(≥F3)的效能,其受试者工作特征曲线下面积(area under receiver operating characteristic curve,AUROC)为0.94,与肝活检的符合率为90.45%。在诊断肝硬化(≥F5)时,血清N-聚糖RF-B模型的AUROC为0.97,与肝组织活检的符合率为88.94%。血清N-聚糖模型(RF-A和RF-B)的诊断效能优于肝硬度值测量(liver stiffness measurement,LSM)、基于4因子的纤维化指数(fibrosis index based on the four factors,FIB-4)和天冬氨酸转氨酶与血小板比率指数(aspartate aminotransferase-to-platelet ratio index,APRI)。在ALT水平正常的慢性乙肝患者中,血清N-聚糖模型可作为诊断显著肝纤维化或肝硬化的潜在生物标志物。
