以MoS_(2)修饰的3D打印还原石墨烯气凝胶制备微型超级电容器电极

微型超级电容器(MSCs)具有高的功率密度和卓越的循环性能,广泛的潜在应用,因而受到诸多关注。然而,制备具有高表面电容和能量密度的MSCs电极仍然存在挑战。本研究使用还原石墨烯气凝胶(GA)和二硫化钼(MoS_(2))作为材料,结合3D打印和表面修饰方法成功构建了具有超高表面电容和能量密度的MSCs电极。通过3D打印技术,获得具有稳定宏观结构和GA交联微孔结构的电极。此外,采用溶液法在3D打印电极表面加载MoS_(2)纳米片,进一步提高了材料的电化学性能。具体而言,电极的表面电容达3.99 F cm^(−2),功率密度为194μW cm^(−2),能量密度为1997 mWh cm^(−2),表现出卓越的电化学性能和循环稳定性。这项研究为制备具有高表面电容和高能量密度的微型超级电容器电极提供了一种简单高效的方法,在MSCs电极领域具有重要的参考意义。

Establishment of a Construct-specific Real-time PCR Method for Quantitative Detection of Genetically Modified Maize Line MIR604

In this study, a construct-specific real-time PCR method for quantitative detection of genetically modified maize line MIR604 was established with prim- ers and probes designed based on vector sequence of MIR604 under optimized reaction system and thermal cycling condition. By using the established method, six non-genetically modified crops, genetically modified maize line MIR604 and other non-target genetically modified crops were detected. According to the results, flu- orescence signal could be detected in genomie DNA of MIR604, but other non-genetically modified crops and non-target genetically modified crops exhibited no fluo- rescence signal of MIR604 molecular fragment. Certified reference materials containing 1% MIR604 were detected with the established method and the results indi- cated that the average relative content in the test samples was 1.05%. The sensitivity analysis results indicated that MIR604 nucleic acid fragment with at least five copies could be detected with the established method. In conclusion, the construct-specific real-time PCR method for quantitative detection of genetically modified maize line MIR604 is of high specificity, accuracy and sensitivity, which provides technical support for safety supervision of genetically modified organisms in China.

Evaluation of Uncertainty in Measuring the Content of CaM V35S Promoter in Genetically Modified Soybean,GTS40-3-2,by Real-time Quantitative PCR

[ Objective ] This study aimed to investigate the major contributors to the measurement uncertainty in quantitative analysis of genetically modified ingreclients and improve the quality of quantitative detection of genetically modified components. [ Method] The content of CaMV35S promoter （parameter） in GTS40- 3-2 soybean powder samples was measured to estimate the measurement uncertainty preliminarily. [ Result] Type A uncertainty （uA） ＇ type B uncertainty （uB） and combined standard uncertainty （Uc） were 0.0 004, 0.002 and 0.002, respectively. At a confidence level ofp = 95% and freedom degree of Voff = 3 251, coverage factor k = 1.96, expanded uncertainty U = 0.004. The final measurement result was C = 0.028 ± 0. 004, which was dose to the conventional true value （0.03）. Thus, the measurement uncertainty was relatively small, indicating a high quality of measurement. In this study, uncertainty evaluation indicated that the deviation of micro liquid transfer made the greatest contribution to the measurement uncertainty. [ Cludusion ] The deviation of micro liquid transfer should be reduced to im- prove the quality of measurement.

Uncertainty in Detecting Specific Fragment of Genetically Modified Maize Event NK603 by Real-time Fluorescence Quantitative PCR

In this study, the event-specific real-time fluorescence quantitative PCR method established by Siehuan Academy of Agricultural Sciences was employed to detect the content of flanking fragment （specific fragment） in samples containing 2% genetically modified maize event NK603. The uncertainty of detection results was evaluated based on various uncertainty sources, such as PCR amplification system, data analysis and micropipette. The results showed that A-type uncertainty （ uA ）, B-type uncertainty （ uB ）, combined standard uncertainty （ uC ） and expanded uncertainty （ U95 ） were 0. 000 8,0.1301,0. 001 and 0. 002, respectively; the final detection result was 1.9% ±0.002. Thus, the main uncertainty in detecting flanking fragment of genetically modified maize event NK603 with realtime fluorescence quantitative PCR method was derived from the random effect in the experimental process.

Development of an Event-specific Quantitative PCR for Genetically Modified Maize (Zea mays) Event NK603

[ Objective ] The aim of this study was to develop a quantitative PCR detection method for genetically modified maize event NK603, so as to provide ba- sis for quantitative analysis of event NK603. [ Methods ] A quantitative PCR detection method for genetically modified maize event NK603 was developed using primers and Taqman probe designed according to the flanking sequence of event NK603, which was then adopted to detect the samples containing 2% NK603 stand- ard （with uncertain quantity of 10% ）. [ Results ] The slope of standard curve ranged between -3.6 and -3.1, and the correlation coefficient was higher than 0. 99. The amplification efficiency of this method reached 100.2%, fallen between 90% and 110%. The detected quantity of the experimental sample was 1.9%, closer to the true quantity （2%）. [ Conclusion] This quantitative PCR detection method for genetically modified maize event NK603 is very precise and can be a- dopted in routine testing analysis.

锂离子电池锂盐浓度的研究进展

综述锂盐浓度通过改变溶剂化结构影响电池界面动力学行为的机理,并分别阐述作用于锂盐溶剂化产物结构、Li^(+)传输性能、固体电解质相界面(SEI)膜稳定性及锂离子电池内部副反应的机理。对低浓度电解液的应用前景进行展望。

THE REACTION OF CALCIUM WITH WAZO-1 AND ITS BIOANALYTICAL APPLICATION

Abstract: A Selective new reagent Wazo-1 was prepared. This paper studied the properties of its fluorescence and UV- Spectrum. A new fluorescence method for the determination of clacium is developed. Wazo-1 reacts with calcium. forms calcium complex(Ex=297nm; Em=370nm). The interference of Mg(Ⅱ)has been studied. The reagent has better selectivity to Ca(Ⅱ) in the presence of large amount of Mg(Ⅱ). Fluorescence method has-been used for determination of microamounts of calcium in serum with new reagent Wazo-1.The results obtained are favourable.

Detection of Herbicide-tolerant Canola by Multiplex PCR

[Objective]This study aimed to establish a multiplex PCR detection method of herbicide-tolerant canola.[Method] An endogenous reference gene(CruA) and three exogenous genes(T-CaMV 35 S, P-CaMV 35 S and pat) were selected for multiplex PCR. Specific primers were designed based on national standards or related literature. The annealing temperature, ratio of primer concentration and sensitivity of the established multiplex PCR system were optimized. The optimal multiplex PCR system was verified with known samples. [Result] The experimental results showed that the optimal annealing temperature of multiplex PCR was 58 ℃; the optimal ratio of primer concentration(μmol/L) was T-CaMV 35S: CruA: P-CaMV 35S: pat=0.1: 0.2: 0.2: 0.2;the detection sensitivity of the established multiplex PCR method was 0.3 ng. The amplified bands of known samples were completely consistent with the molecular characteristics. [Conclusion] This study provided a rapid, accurate and effective multiplex PCR technique for detection of herbicide-tolerant canola.