Experimental study on the effect of cryoablation on lung cancer mice based on MAPK/ERK signaling pathway

Objective:To study the regulatory effect of cryoablation on MAPK/ERK signaling pathway in mice with lung adenocarcinoma.Methods:Lewis mouse lung adenocarcinoma cell line was used to establish subcutaneous transplanted tumor model in C57BL/6 mice.Ten mice were randomly divided into two groups:sham operation group and cryoablation group,with 5 mice in each group.The cryoablation group was treated with double circulation-rewarming ablation,and the sham operation group was treated with incision and suture at the transplanted tumor.The tumor tissues were taken 14 days after operation.Detect the effect of cryoablation on MAPK/ERK pathway related proteins by Western blot,such as KRAS,RAF1,MEK1,ERK1/2,P-RAF1,P-MEK1,P-ERK1/2.The expression of KRAS gene was further verified by qRt-PCR.Results:Compared with the sham operation group,the phosphorylated proteins P-RAF1,P-MEK1 and P-ERK1/2 in tumor tissue after cryoablation were decreased(P<0.05),and the key molecule KRAS in MAPK/ERK pathway was decreased in protein and gene expression(P<0.05).Conclusion:Cryoablation can negatively regulate MAPK/ERK signaling pathway by down-regulating KRas expression.

用于桥梁时变可靠度分析的承载力衰减新模型（英文）

目的:提出能够反映劣化桥梁承载力的非增特性以及自相关性的承载力衰减新模型,并利用该模型进行结构可靠度分析。创新点:提出描述劣化桥梁承载力衰减的新的随机过程模型,给出利用实测数据进行模型参数拟合的方法,并利用提出的衰减模型对劣化桥梁进行时变可靠度分析。方法:采用Gamma过程描述承载力衰减的随机过程(公式7),并基于Monte Carlo模拟的方法研究承载力衰减过程的自相关性对可靠度结果的影响。如果将承载力衰减过程假定为完全相关的过程,则会低估结构的失效概率。

aging structures in the presence of incomplete deterioration information

非完整哀减信息下的劣化结构时变可靠度评估 研宄衰减函数的概率分布对结构可靠度的影响；在非完整衰减信息下，对结构的时变可靠度进行评估。 1．假定衰减函数服从5种常见的概卒分靠，讨论可靠度分析结果的差异；2．当衰减函数的概率分布（或各概率分布的杖重）未知时，提出平均可靠度的分析方法。假定承载力是允伞相关的随机过程，扯其概率分伟未知的情况下：（1）假定5种常见的概率分布，对结构可靠度进行分析和比较；（2）将时变可靠发的计算公式Taylor展开，转化为关于衰减函数一阶矩和二阶矩的函数，推导平均可靠度的计算方法（公式18）。1．不同的衰减函数概率分布对结构可靠度的影响显学；2．利用本文提出的方法，在衰减函数概率的分伟（或各概率分布的杖重）未知时，可以得出结构可靠度的合理评估。