The aim of this study was to investigate the chemical compositions of chalk dust and examine the adverse effects of fine chalk particle matters（PM（2.5）） on rat alveolar macrophages（AMs） in vitro.Morphologies and...The aim of this study was to investigate the chemical compositions of chalk dust and examine the adverse effects of fine chalk particle matters（PM（2.5）） on rat alveolar macrophages（AMs） in vitro.Morphologies and element concentrations of chalk particles were analyzed using quantitative energydispersive electron probe X-ray microanalysis（ED-EPMA）.The oxidative response of AMs exposed to chalk PM（2.5） was measured by luminol-dependent chemiluminescence（CL）.The results showed that（1）Chalk dust was mainly composed of gypsum（CaSO4）,calcite（CaCO3）/dolomite（CaMg（CO3）2）,and organic adhesives;（2） Fine chalk particles induced the AM production of CL,which was inhibited by about 90%by diphenyleneiodonium chloride（DPI）.Based on these results,we showed that cytotoxicity of chalk PM（2.5） may be related to the reactive oxygen species（ROS） generation.展开更多
基金supported by grants from the National Natural Science Foundation of China(Nos.21107064,2140509,21175086 and 21177078)by grant from Shanxi Scholarship Council of China(Nos.2013-012 and 2013-016)
文摘The aim of this study was to investigate the chemical compositions of chalk dust and examine the adverse effects of fine chalk particle matters（PM（2.5）） on rat alveolar macrophages（AMs） in vitro.Morphologies and element concentrations of chalk particles were analyzed using quantitative energydispersive electron probe X-ray microanalysis（ED-EPMA）.The oxidative response of AMs exposed to chalk PM（2.5） was measured by luminol-dependent chemiluminescence（CL）.The results showed that（1）Chalk dust was mainly composed of gypsum（CaSO4）,calcite（CaCO3）/dolomite（CaMg（CO3）2）,and organic adhesives;（2） Fine chalk particles induced the AM production of CL,which was inhibited by about 90%by diphenyleneiodonium chloride（DPI）.Based on these results,we showed that cytotoxicity of chalk PM（2.5） may be related to the reactive oxygen species（ROS） generation.
