Knowledge on methanogenic microbial communities associated with the degradation of polycyclic aromatic hydrocarbons(PAHs)is crucial to developing strategies for PAHs bioremediation.In this study,the linkage between th...Knowledge on methanogenic microbial communities associated with the degradation of polycyclic aromatic hydrocarbons(PAHs)is crucial to developing strategies for PAHs bioremediation.In this study,the linkage between the type of PAHs and microbial community structure was fully investigated through 16 S rRNA gene sequencing on four PAH-degrading cultures.Putative degradation products were also detected.Our results indicated that naphthalene(Nap)/2-methylnaphthalene(2-Nap),phenanthrene(Phe)and anthracene(Ant)sculpted different microbial communities.Among them,Nap and 2-Nap selected for similar degrading bacteria(i.e.,Alicycliphilus and Thauera)and methanogens(Methanomethylovorans and Methanobacterium).Nap and 2-Nap were probably activated via carboxylation,producing 2-naphthoic acid.In contrast,Phe and Ant shaped different bacterial and archaeal communities,with Arcobacter and Acinetobacter being Phe-degraders and Thiobacillus Ant-degrader.Methanogenic archaea Methanobacterium and Methanomethylovorans predominated Phe-degrading and Ant-degrading culture,respectively.These findings can improve our understanding of natural PAHs attenuation and provide some guidance for PAHs bioremediation in methanogenic environment.展开更多
Polycyclic aromatic hydrocarbons(PAHs)often occur in oil-contaminated soil,coke wastewater and domestic sludge;however,associated PAH degraders in these environments are not clear.Here we evaluated phenanthrene degrad...Polycyclic aromatic hydrocarbons(PAHs)often occur in oil-contaminated soil,coke wastewater and domestic sludge;however,associated PAH degraders in these environments are not clear.Here we evaluated phenanthrene degradation potential in the mixed samples of above environments,and obtained a methanogenic community with different microbial profile compared to those from sediments.Phenanthrene was efficiently degraded(1.26 mg/L/d)and nonstoichiometric amount of methane was produced simultaneously.16S rRNA gene sequencing demonstrated that bacterial populations were mainly associated with Comamonadaceae Nocardiaceae and Thermodesulfobiaceae,and that methanogenic archaea groups were dominated by Methanobacterium and Methanothermobacter.Substances such as hexane,hexadecane,benzene and glucose showed the most positive effects on phenanthrene degradation.Substrate utilization tests indicated that this culture could not utilize other PAHs.These analyses could offer us some suggestions on the putative phenanthrene-degrading microbes in such environments,and might help us develop strategies for the removal of PAHs from contaminated soil and sludge.展开更多
基金supported by the National Natural Science Foundation Projects (Nos. 41573065 and 41773082)the Key Project of Natural Science Foundation of China (No. 21337001)the National Water Pollution Control and Treatment Science and Technology Major Project (No. 2017ZX07202002)
文摘Knowledge on methanogenic microbial communities associated with the degradation of polycyclic aromatic hydrocarbons(PAHs)is crucial to developing strategies for PAHs bioremediation.In this study,the linkage between the type of PAHs and microbial community structure was fully investigated through 16 S rRNA gene sequencing on four PAH-degrading cultures.Putative degradation products were also detected.Our results indicated that naphthalene(Nap)/2-methylnaphthalene(2-Nap),phenanthrene(Phe)and anthracene(Ant)sculpted different microbial communities.Among them,Nap and 2-Nap selected for similar degrading bacteria(i.e.,Alicycliphilus and Thauera)and methanogens(Methanomethylovorans and Methanobacterium).Nap and 2-Nap were probably activated via carboxylation,producing 2-naphthoic acid.In contrast,Phe and Ant shaped different bacterial and archaeal communities,with Arcobacter and Acinetobacter being Phe-degraders and Thiobacillus Ant-degrader.Methanogenic archaea Methanobacterium and Methanomethylovorans predominated Phe-degrading and Ant-degrading culture,respectively.These findings can improve our understanding of natural PAHs attenuation and provide some guidance for PAHs bioremediation in methanogenic environment.
基金supported by the National Natural Science Foundation of China(Grant Nos.41573065 and 41773082)the Key Project of Natural Science Foundation of China(Grant No.21337001)the National Water Pollution Control and Treatment Science and Technology Major Project(Grant No.2017ZX07202002).
文摘Polycyclic aromatic hydrocarbons(PAHs)often occur in oil-contaminated soil,coke wastewater and domestic sludge;however,associated PAH degraders in these environments are not clear.Here we evaluated phenanthrene degradation potential in the mixed samples of above environments,and obtained a methanogenic community with different microbial profile compared to those from sediments.Phenanthrene was efficiently degraded(1.26 mg/L/d)and nonstoichiometric amount of methane was produced simultaneously.16S rRNA gene sequencing demonstrated that bacterial populations were mainly associated with Comamonadaceae Nocardiaceae and Thermodesulfobiaceae,and that methanogenic archaea groups were dominated by Methanobacterium and Methanothermobacter.Substances such as hexane,hexadecane,benzene and glucose showed the most positive effects on phenanthrene degradation.Substrate utilization tests indicated that this culture could not utilize other PAHs.These analyses could offer us some suggestions on the putative phenanthrene-degrading microbes in such environments,and might help us develop strategies for the removal of PAHs from contaminated soil and sludge.
