[Objective] The paper was to observe the effects of Guangxi Hepu pearl hydrolysate on proliferation and apoptosis of human hepatic stellate cells. [Method] The inhibition rate against the proliferation of human hepati...[Objective] The paper was to observe the effects of Guangxi Hepu pearl hydrolysate on proliferation and apoptosis of human hepatic stellate cells. [Method] The inhibition rate against the proliferation of human hepatic stellate cells was determined by MTT colorimetry after co-incubation with pearl hydrolysate for 48 h. The percentage of early and late apoptotic cells was determined by flow cytometry(Annexin V-FITC/PI staining). [Result] Low, medium and high concentrations of pearl hydrolysate could inhibit the proliferation of human hepatic stellate cells, and the inhibition rates at 48 h were 12.4%, 27.4% and 37.8%, respectively. The low, medium and high concentrations of pearl hydrolysate could induce the apoptosis of cells. The percentages of total apoptotic cells(early apoptotic + late apoptotic) at 48 h were 8.97%, 16.09% and 26.98%, respec-tively. [Conclusion] Pearl hydrolysate can inhibit the proliferation of human hepatic stellate cells and induce the apoptosis of these cells, which may be the mechanism of anti-hepatic fibrosis effect of pearl hydrolysate. These effects are dose-dependent. It is also found that the above cellular effects of high dose pearl hydrolysate are stronger than those of colchicine and compound Biejiaruangan tablets.展开更多
Dear Editor,The coronavirus infectious disease 2019(COVID-19)outbreak is seriously endangering human health.Most patients with severe COVID-19 are characterized by sustained cytokine production and hyper-inflammation,...Dear Editor,The coronavirus infectious disease 2019(COVID-19)outbreak is seriously endangering human health.Most patients with severe COVID-19 are characterized by sustained cytokine production and hyper-inflammation,which is known as cytokine storm syndrome.1-3 Elaborating the anti-inflammatory response is crucial to these patients,and interieukin-6(IL6)inhibitors and steroids have been recommended in clinical practice.1 However,after the cytokine storm phase,the host immune response to sepsis may develop into a protracted immunosuppressive phase.展开更多
Dear Editor,The coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a major global health threat.Mortality is predominantly caused by severe respiratory f...Dear Editor,The coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a major global health threat.Mortality is predominantly caused by severe respiratory failure related to interstitial pneumonia in both lungs and acute respiratory distress syndrome.Recently,genome-wide association studies(GWASs)have identified chromosome 3p21.31(sentinel variant:rsl 1385942)to be associated with both severe and general affected COVID-19 cases.展开更多
基金Supported by National Natural Science Foundation of China (81960761, 82060825, 81960751)Natural Science Foundation of Guangxi Province (2020JJA140257)+1 种基金Cultivation Plan of Thousand Backbone Teachers in Guangxi Institutions of Higher LearningGuangxi Specialized Experts Post Fund of "Beibu Gulf Marine Medicine Research and Development"&Guangxi First-class Discipline:Traditional Chinese Medicine (Scientific Research of Guangxi Education Department [2018] No.12)。
文摘[Objective] The paper was to observe the effects of Guangxi Hepu pearl hydrolysate on proliferation and apoptosis of human hepatic stellate cells. [Method] The inhibition rate against the proliferation of human hepatic stellate cells was determined by MTT colorimetry after co-incubation with pearl hydrolysate for 48 h. The percentage of early and late apoptotic cells was determined by flow cytometry(Annexin V-FITC/PI staining). [Result] Low, medium and high concentrations of pearl hydrolysate could inhibit the proliferation of human hepatic stellate cells, and the inhibition rates at 48 h were 12.4%, 27.4% and 37.8%, respectively. The low, medium and high concentrations of pearl hydrolysate could induce the apoptosis of cells. The percentages of total apoptotic cells(early apoptotic + late apoptotic) at 48 h were 8.97%, 16.09% and 26.98%, respec-tively. [Conclusion] Pearl hydrolysate can inhibit the proliferation of human hepatic stellate cells and induce the apoptosis of these cells, which may be the mechanism of anti-hepatic fibrosis effect of pearl hydrolysate. These effects are dose-dependent. It is also found that the above cellular effects of high dose pearl hydrolysate are stronger than those of colchicine and compound Biejiaruangan tablets.
基金We thank the Beijing Talents Foundation(2017000021223ZK30)Beijing Lab Foundation+1 种基金Special Funds for COVID-19 Prevention and Control of West China Hospital of Sichuan University(HX-2019-nCoV-049)the National Key Research and Developm ent Program of China(SQ2020YFF0426498).
文摘Dear Editor,The coronavirus infectious disease 2019(COVID-19)outbreak is seriously endangering human health.Most patients with severe COVID-19 are characterized by sustained cytokine production and hyper-inflammation,which is known as cytokine storm syndrome.1-3 Elaborating the anti-inflammatory response is crucial to these patients,and interieukin-6(IL6)inhibitors and steroids have been recommended in clinical practice.1 However,after the cytokine storm phase,the host immune response to sepsis may develop into a protracted immunosuppressive phase.
基金supported by Sichuan Science and Technology Program(no.2019YFH0137,to Y.Y.).
文摘Dear Editor,The coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a major global health threat.Mortality is predominantly caused by severe respiratory failure related to interstitial pneumonia in both lungs and acute respiratory distress syndrome.Recently,genome-wide association studies(GWASs)have identified chromosome 3p21.31(sentinel variant:rsl 1385942)to be associated with both severe and general affected COVID-19 cases.
