Attenuated plasmodium sporozoite expressing MAGE-A3 induces antigen-specific CD8+ T cell response against lung cancer in mice

Objective: Cancer vaccines that rely on tumor antigen-specific CD8^+ T cell responses, are promising anti-cancer adjuvant immunotherapies. This study investigated whether genetically attenuated Plasmodium sporozoite(GAS) could be used as a novel vector to induce antigen-specific CD8^+ T cell responses against lung cancer.Methods: We constructed GAS/MAGE-A3, a recombinant GAS engineered to express the lung cancer-specific antigen, melanomaassociated antigen 3(MAGE-A3), and assessed its therapeutic effects against lung cancer.Results: Robust parasite-specific CD8αlow CD11ahigh and CD49dhigh CD11ahigh CD4^+ T cell responses as well as a MAGE-A3-specific CD8^+ T cell response were induced in GAS/MAGE-A3-immunized mice. Adoptive transfer of GAS/MAGE-A3-induced CD8^+ T cells from HLA-A2 transgenic mice into lung cancer-bearing nude mice inhibited tumor growth and prolonged survival.Conclusions: These findings demonstrate that GAS/MAGE-A3 induces a strong MAGE-A3-specific CD8^+ T cell response against lung cancer in vivo, and indicate that GAS is a novel and efficacious antigen delivery vector for antitumor immunotherapy.

基于嫦娥五号月球样品的月壤残余内摩擦角预测

随着人类探月工程的快速发展,月球基地建设以及月表资源开发利用有望更快实现,合理预测月壤工程力学性质对于未来深层次探月工程意义重大,我国嫦娥五号返回月球样品为研究月壤工程力学特性提供了直接的实测材料。然而,月球样品极其珍贵,难以满足传统土工试验测试.为了应对这一科学挑战,本研究从无损分析月球样品颗粒属性入手,使用高精度X-射线μCT扫描、三维白光干涉、原子力显微镜等无损测试手段,分析了不同类型月壤颗粒的三维多尺度形态、弹性力学和摩擦属性等指标.在此基础上,基于颗粒材料宏微观物理力学理论和数值模型尝试预测了嫦娥五号采样处月壤残余内摩擦角。本研究为基于月球样品信息跨尺度分析月壤工程力学性质提供了可行思路。

生态系统的多稳态与突变

许多生态系统可能在短时间内发生难以预料的状态突变,其中一些生态系统突变的机理可以用多稳态理论进行解释。近年来生态系统的多稳态和突变现象及其机理吸引了研究者和管理者的广泛关注。本文重点对生态系统多稳态的理论基础、识别方法及稳态转换发生的早期预警信号进行综述,并基于典型生态系统过程对现实世界中可能观测到的稳态转换进行实例分析,最后对多稳态概念框架和理论应用中的潜在争议进行讨论,以期为非线性生态系统动态的理论研究、管理实践和生物多样性保护等提供参考。