扩大碟形术加带血管蒂腓骨游离移植治疗胫骨慢性骨髓炎

目的:探索扩大碟形术加带血管蒂腓骨游离移植治疗胫骨慢性骨髓炎的可行性和疗效。方法:12例胫骨慢性骨髓炎患者行胫骨骨髓炎病灶清除、扩大碟形术,取对侧带血管蒂腓骨游离移植,修复患侧胫骨切除后缺损,置冲洗引流管。结果:经2~3年随访,优5例,良6例,中1例,绝大部分病例胫骨碟形化部经重建修复后在13~18月内满意愈合,恢复行走功能,少部分病例术后切口有轻微感染。经随访发现,随着骨缺损部逐渐愈合,移植来的腓骨会逐渐塑形、增粗,并与受区胫骨融为一体。结论:扩大碟形术加带血管蒂腓骨游离移植治疗胫骨慢性骨髓炎具有可行性和较满意之疗效,强调骨髓炎手术适应症的正确把握和感染病灶清除的适当彻底,须注意围手术期全程的规范化管理。

Hepatitis B virus infection and replication in primarily cultured human fetal hepatocytes

AIM: To investigate the infection and replication of hepatitis B virus (HBV) in primarily cultured human fetal hepatocytes (HFHs). METHODS: The human fetal hepatocytes were cultured in serum-free medium, HBV-positive serum was added into the medium to study the susceptibility of hepatocytes to HBV infection. The supernatant was collected for ELISA assay of HBsAg and HBeAg, and quantitative fluorescence PCR for HBV-DNA assay daily. Albumin and HBcAg, CK8 and CK18 expressions were detected by immunohistochemistry in cultured hepatocytes. Content of lactate dehydrogenate (LDH) was measured to find out the integrity of the cell membrane. RESULTS: A stable hepatocyte culture system was established. HBV could infect the hepatocytes and replicate, and HBcAg expression could be detected by immunohistochemistry in hepatocyte-like cells. HBV- DNA in the supernatant could be detected from d 2 to d 18 and HBsAg and HBeAg were positive on d 3-d 18 after HBV infection. HBV in medium increased from d 0 to d 6 and subsequently decreased as the cells were progressively loosing their hepatocyte phenotypes. CONCLUSION: HBV could infect human fetal hepato- cytes and replicate. This in vitro model allowed a detailed study on early events associated with human HBV entry into cells and subsequent replication.

SULFATION OF PACHYMAN WITH CHLOROSULFONIC ACID USING THE IMPROVED WOLFROM METHOD

High purity polysaccharide of pachyman was isolated from the powder of Poria cocos sclerotium with an yield of 77.8%. The intrinsic viscosity of polysaccharide was found to be 78.95 mL/g in DMSO solution at 25℃. The isolated polysaccharide was reacted with chlorosulfonic acid to obtain pachyman sulfate using the improved Wolfrom method. The results of the orthogonality experiment on the sulfation reaction identified that the effectiveness of the reaction conditions on the degree of sulfation and the value of intrinsic viscosity is in the following order： molar ratio of chlorosulfonic acid to glucoside （3-5） 〉 reaction temperature （60-80℃） 〉 reaction time （1 2 h）, The kinetic studies of the pachyman sulfation indicated that the hydrolysis is accompanied with the sulfation process. The decrease in intrinsic viscosity of the sulfated pachyman is proportional to the increase in the degree of sulfation under the mild reaction conditions of 〈 80℃, chlorosulfonic acid/glucoside mole ratio 〈 5, and reaction time 〈 2 h. Beyond the above reaction conditions, excessive loss of --OH group occurs during hydrolysis. The NMR results indicated a complete sulfation on C-6 and a partial sulfation on the C-2 and C-4 of glucoside.

Rare case of drain-site hernia after laparoscopic surgery and a novel strategy of prevention:A case report

BACKGROUND Trocar site hernia(TSH)is a rare but potentially dangerous complication of laparoscopic surgery,and the drain-site TSH is an even rarer type.Due to the difficulty to diagnose at early stages,TSH often leads to a delay in surgical intervention and eventually results in life-threatening consequences.Herein,we report an unusual case of drain-site TSH,followed by a brief literature review.Finally,we provide a novel,simple,and practical method of prevention.CASE SUMMARY A 54-year-old female patient underwent laparoscopic subtotal hysterectomy and bilateral adnexectomy for uterine fibroids 8 d ago in another hospital.She was admitted to our hospital with a 2-d history of intermittent abdominal pain,nausea,vomiting,and abdominal enlargement with an inability to pass stool and flatus.The emergency computed tomography scan revealed the small bowel herniated through a 10 mm trocar incision,which was used as a drainage port,with diffuse bowel distension and multiple air-fluid levels with gas in the small intestines.She was diagnosed with drain-site strangulated TSH.The emergency exploratory laparotomy confirmed the diagnosis.A herniorrhaphy followed by standard intestinal resection and anastomosis were performed.The patient recovered well after the operation and was discharged on postoperative day 8 and had no postoperative complications at her 2-wk follow-up visit.CONCLUSION TSH must be kept in mind during the differential diagnosis of post-laparoscopic obstruction,especially after the removal of the drainage tube,to avoid the serious consequences caused by delayed diagnosis.Furthermore,all abdomen layers should be carefully closed under direct vision at the trocar port site,especially where the drainage tube was placed.Our simple and practical method of prevention may be a novel strategy worthy of clinical promotion.

Refining murine heterotopic heart transplantation:A model to study ischemia and reperfusion injury in donation after circulatory death hearts

Heart transplantation is a lifesaving procedure,which is limited by the availability of donor hearts.Using hearts from donors after circulatory death,which have sustained global ischemia,requires thorough studies on reliable and reproducible models that developing researchers may not have mastered.By combining the most recent lit-erature and our recommendations based on observations and trials and errors,the methods here detail a sound in vivo heterotopic heart transplantation model for rats in which protective interventions on the ischemic heart can be studied,and thus al-lowing the scientific community to advance organ preservation research.Knowledge gathered from reproducible animal models allow for successful translation to clinical studies.

ADSORPTION OF LATERALLY INTERACTING 1-NAPHTHOL/1-NAPHTHYL-AMINE MIXTURES ON NONPOLAR SURFACES FROM AQUEOUS SOLUTIONS

The binary adsorption behavior of 1-naphthol/1-naphthylamine mixtures in water on nonpolar adsorbent Amberlite XAD4 was investigated at 293 K, 303 K and 313 K, respectively. The experimental uptakes of 1-naphthol and 1-naphthylamine in all binary-component systems of different molar ratios were obviously higher than the corresponding uptakes predicted by the extended Langmuir model, assuming no interaction between the adsorbed molecules of the two components. This phenomenon was attributed to the cooperative adsorption effect arising from the hydrogen bonding interaction between 1-naphthol and 1-naphthylamine molecules. A modified extended Langmuir model was proposed to describe the binary adsorption behavior by means of introducing a fitting parameter related with the cooperative adsorption effect of the adsorbates.

Research Status of Serum Pharmacology and Serum Pharmacochemistry of Traditional Chinese Medicine

Serum pharmacochemistry of traditional Chinese medicine has become a more accurate and rapidly developing reasonable way to analyze the effective material basis of traditional Chinese medicine in recent years. Through this method, we can select very complex Chinese medicine components and clarify the effective substances consistent with the main functions, and then separate and purify effective ingredients in traditional Chinese medicine, so as to explain the material basis of the drug effect and prove the rationality and principle of action of Chinese medicine compounds. It can show the multi-component and multi-target characteristic of traditional Chinese medicine, and after analyzing the migrating components of serum, we can explore the direct effective substances that act on the patient’s body, so as to accelerate and accurately complete the in-depth study of the effective substances of Chinese medicine.

Time-dependent matrix metalloproteinases and tissue inhibitor of metalloproteinases expression change in fusarium solani keratitis

AIM： To investigate matrix metalloproteinases（MMPs）and tissue inhibitor of metalloproteinases（TIMPs）expression during the progress of fusarium solani（F.solani） keratitis in a rat model.· METHODS： A rat model of F.solani keratitis was produced using corneal scarification and a hand-made contact lens. MMPs and TIMPs expressiond were explored in this rat model of F.solani keratitis using real-time polymerase chain reaction（PCR） and DIF.GM6001（400 μmol/m L） was used to treat infected corneas. The keratitis duration, amount and area of corneal neovascularization（CNV） were evaluated.·RESULTS： MMP-3 expression was 66.3 times higher in infected corneas compared to normal corneas. MMP-8,-9,and-13 expressions were significantly upregulated in the mid-period of the infection, with infected- to-normal ratios of 4.03, 39.86, and 5.94, respectively. MMP-2 and-7expressions increased in the late period, with the infected-to-normal ratios of 5.94 and 16.22, respectively.TIMP-1 expression was upregulated in the early period,and it was 43.17 times higher in infected compared to normal corneas, but TIMP-2,-3, and-4 expressions were mildly downregulated or unchanged. The results of DIF were consistent with the result of real-time PCR.GM6001, a MMPs inhibitor, decreased the duration of F.solani infection and the amount and area of CNV.·CONCLUSION： MMPs and TIMPs contributed into the progress of F.solani keratitis.

Pharmacological Action and Research Progress of Traditional Chinese Medicine on Liver Injury

According to the different types of liver damage,there should also be different Chinese medicine and dose for different degrees of treatment and protection.This paper studies the Chinese medicine treatment of different types of liver injury,and is expected to provide a reference for the further application of Chinese medicine in liver injury.

微生物检验在临床应用中的质量控制分析

目的探究微生物检验在临床应用中的质量控制策略以及临床控制效果。方法选取2016年2月-2017年2月我院ICU病房中收治的480例呼吸道感染患者作为本次临床研究资料,采用随机方式将患者分为对照组与观察组,每组患者均有240例,观察组患者在微生物检验前实施质量控制,并根据微生物检验结果为患者提供对症治疗,对照组患者则在接受常规微生物检验后,接受对症治疗,对比两组患者检验结果与治疗效果。结果与对照组相比,观察组患者致病菌类型以及检出率更高,两组检验差异显著,具有统计学意义(P<0.05);观察组患者在质量控制微生物检验的对症治疗支持下,总有效率达到98.8%,对照组治疗总有效率仅为85.4%,两组患者治疗差异显著(P<0.05)。结论在实施微生物检验前进行质量控制,有助于查明致病原因并提高临床检验准确率,提高治疗针对性与有效性。

Pressure-induced phase transitions in the ZrXY(X=Si,Ge,Sn;Y=S,Se,Te)family compounds

Pressure is an effective and clean way to modify the electronic structures of materials,cause structural phase transitions and even induce the emergence of superconductivity.Here,we predicted several new phases of the Zr XY family at high pressures using the crystal structures search method together with first-principle calculations.In particular,the Zr Ge S compound undergoes an isosymmetric phase transition from P4/nmm-I to P4/nmm-II at approximately 82 GPa.Electronic band structures show that all the high-pressure phases are metallic.Among these new structures,P4/nmm-II Zr Ge S and P4/mmm Zr Ge Se can be quenched to ambient pressure with superconducting critical temperatures of approximately 8.1 K and 8.0 K,respectively.Our study provides a way to tune the structure,electronic properties,and superconducting behavior of topological materials through pressure.

EFFECT OF WEAK INTERACTIONS ON PHENOL ADSORPTION FROM AQUEOUS SOLUTIONS BY AMINATED POLYMERIC ADSORBENTS

Adsorption behaviors of phenol from aqueous solutions have been investigated in batch systems at 303 K and 318 K respectively, using hypercrosslinked polymeric adsorbent （CHA111）, aminated hypercrosslinked polymeric adsorbents （NDA101, NDA103, NDA105） and weakly basic polymeric adsorbent （D301） with a view to studying the effect of hydrogen bonding and Van der Waals interactions between adsorbate and the adsorbent. All adsorption isotherms can be well fitted by Langmuir and Freundlich equations. Compared with D301 driven by hydrogen bonding interaction only and CHA111 driven by Van der Waals interaction only, phenol adsorption on aminated adsorbents driven by both hydrogen bonding and Van der Waals interactions were apparently different, i.e., negative effect for NDA105, positive effect for NDA101 and synergistic effect for NDA103. In this synergistic action, some weak interactions would contribute more or less to the adsorption than they work individually.

Preparation of hybridizing zeolitic imidazolate frameworks with carboxymethylcellulose for adsorption separation of n-hexane/3-methylpentane

A novel ZIF-8-CMC hybrid material was fabricated from the hybridization of ZIF-8 and carboxymethylcellulose(CMC) by impregnation method for n-hexane/3-methylpentane separation.The surface properties of ZIF-8 were tailored by introducing CMC into ZIF-8 nanoparticles.In this work,adsorption separation of n-hexane(nHEX) and 3-methylpentane(3 MP) on ZIF-8-CMC were investigated by batch vapor-phase adsorption and liquid-phase breakthrough adsorption.The adsorption selectivity of nHEX/3 MP reversed from preferable adsorption of nHEX to preferable adsorption of 3 MP upon the increasing of CMC containing in the hybrid materials.As the temperature increases,the adsorption amounts of nHEX and 3 MP decrease.With the increasing of CMC contents,the nHEX uptake decreased,the uptake capacity of 3 MP increased gradually.For liquid-phase breakthrough adsorption,the dynamic adsorption capacity of nHEX also decreased with the increasing of temperature.

Intracortical screw insertion plus limited open reduction in treating type 31A3 irreducible intertrochanteric fractures in the elderly

BACKGROUND In most elderly patients with intertrochanteric fractures,satisfactory fracture reduction can be achieved by closed reduction using a traction table.However,intertrochanteric fractures cannot achieve satisfactory reduction in a few patients,which is called irreducible intertrochanteric fractures.Especially for type 31A3 irreducible intertrochanteric fractures,limited open reduction of the broken end with different intraoperative reduction methods is required to achieve satisfactory reduction and fixation.AIM To discuss clinical efficacy of intracortical screw insertion plus limited open reduction in type 31A3 irreducible intertrochanteric fractures in the elderly.METHODS A retrospective analysis was performed on 23 elderly patients with type 31A3 irreducible intertrochanteric fractures(12 males and 11 females,aged 65-89-yearsold)who received treatment at the orthopedics department.After type 31A3 irreducible intertrochanteric fractures were confirmed by intraoperative C-arm,all of these cases received intracortical screw insertion plus limited open reduction in the broken end with intramedullary screw internal fixation.The basic information of surgery,reduction effects,and functional recovery scores of the hip joint were assessed.RESULTS All patients were followed up for 13.8 mo on average.The operation time was 53.8±13.6 min(40-95 min).The intraoperative blood loss was 218.5±28.6 mL(170-320 mL).The average number of intraoperative X-rays was 22.8±4.6(18-33).The average time to fracture union was 4.8±0.7 mo.The reduction effect was assessed using Kim’s fracture reduction evaluation.Twenty cases achieved grade I fracture reduction and three cases grade II fracture reduction.All of them achieved excellent or good fracture reduction.Upon the last follow-up,the functional recovery scores score was 83.6±9.8,which was not significantly different from the functional recovery scores score(84.8±10.7)before the fracture(t=0.397,P=0.694).CONCLUSION With careful preoperative preparation,intracortical screw insertion plus limited open reduction contributed to high-quality fracture reduction and fixation.Good clinical outcomes were achieved without increasing operation time and intraoperative blood loss.

Extraction Process of Basil Essential Oil by Steam Distillation

[Objectives] To optimize volatile oil extraction from Ocimum basilicum with the water vapor distillation. [Methods] An orthogonal design was carried out to determine the volume of volatile oil by 3 factors: the immersion time, distillation time, amount of water. The ratio of the oil in inclusion complex was used to evaluate the technology based on the orthogonal design. [Results] The best volatile oil extraction condition was to add 400 mL of water into the mixture of crude drugs, and to extract the herbal medicine for 2 h with advanced soaking for 6 h with water. [Conclusions] The process is stable, reasonable, and feasible.

Anti-inflammatory Effects of Volatile Oil of Ocimum basilicum L. in Bozhou and Its Mechanisms

[Objectives] The research aimed to study the anti-inflammatory effects of volatile oil from Ocimum basilicum L. in Bozhou through the experimental animal model of inflammation.[Methods] Xylene-induced mouse ear swelling model and carrageenan-induced mouse paw swelling model were used to compare the anti-inflammatory effects of volatile oil from O. basilicum L.[Results] The extremely high, high, medium, and low doses of volatile oil from O. basilicum L. had a certain inhibitory effect on the ear swelling of mice induced by xylene ( P <0.05), and the inhibitory effect of high-dose group on the ear swelling of mice was better. The swollen degree of the mice s toes in the different dose groups of volatile oil from O. basilicum L. was significantly lower ( P <0.05), and the high-dose and middle-dose groups had better inhibition.[Conclusions] The volatile oil from O. basilicum L. in Bozhou had a significant inhibitory effect on xylene-induced mouse ear swelling and carrageenan-induced mouse paw swelling. It proved and clarified the anti-inflammatory effects of volatile oil from O. basilicum L. in Bozhou.

Optimal Method of Extraction Process of Flavonoids from Ginkgo biloba

[Objectives] To optimize the extraction of total flavone in folium Ginkgo biloba leaves.[Methods] Using the ultrasonic extraction method, the three levels of maximum impact were selected by each factor selection, and the optimal extraction technology was obtained by box-benhnken test design analysis.[Results] After analyzing the results, the optimal process was as follows: a time of 28.89 min and liquid/material ratio of 31.38∶ 1, the temperature was 40.74℃. In this way, NaNO 2-Al (NO 3) 3-NaOH was used as color developing agent, and the theoretical extraction of flavone in ginkgo leaf can reach 11.74 mg/g.[Conclusions] This method can be used to extract total flavonoids from G. biloba leaves with simple operation, low cost and good repeatability.

Strategies for Informatization Construction of Continuing Education Management in Higher Vocational Colleges:Taking Pharmacology Courses as an Example

In the era of rapid development of informatization,the informatization construction of national higher vocational colleges has been in the period of application deepening.China's education system is deepening to the development of mass education,and higher vocational colleges'adult continuing education plays a great role for the realization of education popularization.With the continuous expansion of continuing education enrollment scale,the diversified development of education mode,the higher complexity of continuing education management and the increase of management workload,the traditional management method has gradually become unapplicable.Higher vocational colleges can greatly improve the management level by promoting the informatization of education management according to their own professional or regional advantages.

Review on the Chemical Composition and Pharmacological Action of Ocimum basilicum

Ocimum basilicum has a history of several thousand years,the plant resources are rich,and there are many kinds of chemical constituents and pharmacological activity. In the face of the human body,it has a broad role in many ways. O. basilicum has a long history of folk application and research,and very high officinal,edible and economic value. Combining with modern analysis and detection technology,the effective parts of O. basilicum are studied and utilized. The discovery of new drugs will have important practical significance for the development and application of O. basilicum.

Research Progress on Chemical Composition and Clinical Effect of Ocimum basilicum

Ocimum basilicum is a common herb well known for its importance for ornamental and therapeutic purposes. Chemical components that have been isolated from O. basilicum plants include terpenoids,alkaloids,flavonoids,tannic acid,saponin glycosides,and ascorbic acid.O. basilicum has liver protection,immune regulation,anti-hyperglycemia,hypolipidemic,anti-toxin,anti-inflammatory,anti-bacterial and anti-fungal effects. This paper aims to review the latest progress in phytochemical research and pharmacological research of O. basilicum,which will assist various practitioners and clinicians in understanding the traditional and modern uses of O. basilicum.