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Porous Calcium Phosphate Ceramic Scaffolds for Tissue Engineering 被引量:6
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作者 L Di Silvio N Gurav +1 位作者 J Merry r sambrook 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2005年第B12期13-15,共3页
This study examined the biological response of two porous calcium phosphate ceramics, hydroxyapntite ( HA ) and hydroxyapaptite/β-tricalcium phosphate ( HA/β- TCP ) scaffolds. Three different cell types , a huma... This study examined the biological response of two porous calcium phosphate ceramics, hydroxyapntite ( HA ) and hydroxyapaptite/β-tricalcium phosphate ( HA/β- TCP ) scaffolds. Three different cell types , a human osteoblastic cell line ( HOS ) , primary human osteoblasts (HOB) and human mesenehymal stem cells (MSCs), were used to examine biocompatibility and osteoinductive capacity. The experimental results showed both materials were highly biocompatible and proliferation was significantly greater on pure HA ( P 〈 0.01 ), with a peak in proliferation at day 7. Protein levels were significantly higher ( P 〈 0.05) than the control Thermanox( TMX ( tm) ) for both test materials. Osteoinduction of MSCs was observed on both test materials, with cells seeded on HA/ β-TCP showing greater alkaline phosphatase activity compared to HA alone, indicating an enhancement in osteoinductive property. Both materials show good potential for use as tissue engineered scaffolds. 展开更多
关键词 calcium phosphate ceramics mesenchymal stem cells bone graft OSTEOINDUCTION
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