期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
A pilot study of the modulation of sirtuins on arylamine N-acetyltransferase 1 and 2 enzymatic activity 被引量:7
1
作者 Eneida Turiján-Espinoza rául alejandro salazar-gonzález +4 位作者 Edith Elena Uresti-rivera Gloria Estela Hernández-Hernández Montserrat Ortega-Juárez rosa Milán Diana Portales-Pérez 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2018年第2期188-199,共12页
Arylamine N-acetyltransferase(NAT; E.C. 2.3.1.5) enzymes are responsible for the biotransformation of several arylamine and hydrazine drugs by acetylation. In this process, the acetyl group transferred to the acceptor... Arylamine N-acetyltransferase(NAT; E.C. 2.3.1.5) enzymes are responsible for the biotransformation of several arylamine and hydrazine drugs by acetylation. In this process, the acetyl group transferred to the acceptor substrate produces NAT deacetylation and, in consequence, it is susceptible of degradation. Sirtuins are protein deacetylases, dependent on nicotine adenine dinucleotide,which perform post-translational modifications on cytosolic proteins. To explore possible sirtuin participation in the enzymatic activity of arylamine NATs, the expression levels of NAT1, NAT2,SIRT1 and SIRT6 in peripheral blood mononuclear cells(PBMC) from healthy subjects were examined by flow cytometry and Western blot. The in situ activity of the sirtuins on NAT enzymatic activity was analyzed by HPLC, in the presence or absence of an agonist(resveratrol) and inhibitor(nicotinamide) of sirtuins. We detected a higher percentage of positive cells for NAT2 in comparison with NAT1, and higher numbers of SIRT1t cells compared to SIRT6 in lymphocytes. In situ NAT2 activity in the presence of NAM inhibitors was higher than in the presence of its substrate, but not in the presence ofresveratrol. In contrast, the activity of NAT1 was not affected by sirtuins. These results showed that NAT2 activity might be modified by sirtuins. 展开更多
关键词 Arylamine N-acetyltransferase NAT SIRTUINS Peripheral blood mononuclear cells NICOTINAMIDE RESVERATROL
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部