Acaricidal activity of Boenninghausenia sessilicarpa against Panonychus cirri was tested in the laboratory. Four solvents were used to prepare crude extracts, petroleum ether, chloroform, ethyl acetate, and ethanol, a...Acaricidal activity of Boenninghausenia sessilicarpa against Panonychus cirri was tested in the laboratory. Four solvents were used to prepare crude extracts, petroleum ether, chloroform, ethyl acetate, and ethanol, among which ethanol was the most effective one. The results suggested that ethanol extracts of B. sessilicarpa had eminent acaricidal and ovicidal activities. Concentrated extracts were prepared using petroleum ether, chloroform, ethyl acetate, or distilled water as solvent. Mite mortality rates in the concentrated extracts by petroleum ether, chloroform, or distilled water were significantly lower than those by ethyl acetate. The LC50 values of eggs and female mites were 0.7639 and 1.1033 mg mL^-1, respectively. After liquid chromatography and thin layer chromatography, the concentrated extracts were separated into 14 groups of fractions and further tests for their acaricidal and ovicidal activities were conducted. Fraction 2 was found to possess higher acaricidal and ovicidal activities. The mortality of eggs and adult mites were 85.83 and 63.07%, respectively. Moreover, fraction 2 showed moderate oviposition inhibition effect (0.8795) against P. citri when the used dose was higher than 2.5 mg mL^-1展开更多
Neoseiulus barker/Hughes is one of the best biological control products. The paper reviewed taxonomic status, biological characteristics, large-scale breeding and application research status of N. barkeri, so as to pr...Neoseiulus barker/Hughes is one of the best biological control products. The paper reviewed taxonomic status, biological characteristics, large-scale breeding and application research status of N. barkeri, so as to provide scientific basis for further research and application of N. barkeri.展开更多
基金supported by the Special Public Sector Research of China (nyhyzx07-057)the National Key Technology R&D Program of China(2007BAD47B04, 2008BAD92B08)
文摘Acaricidal activity of Boenninghausenia sessilicarpa against Panonychus cirri was tested in the laboratory. Four solvents were used to prepare crude extracts, petroleum ether, chloroform, ethyl acetate, and ethanol, among which ethanol was the most effective one. The results suggested that ethanol extracts of B. sessilicarpa had eminent acaricidal and ovicidal activities. Concentrated extracts were prepared using petroleum ether, chloroform, ethyl acetate, or distilled water as solvent. Mite mortality rates in the concentrated extracts by petroleum ether, chloroform, or distilled water were significantly lower than those by ethyl acetate. The LC50 values of eggs and female mites were 0.7639 and 1.1033 mg mL^-1, respectively. After liquid chromatography and thin layer chromatography, the concentrated extracts were separated into 14 groups of fractions and further tests for their acaricidal and ovicidal activities were conducted. Fraction 2 was found to possess higher acaricidal and ovicidal activities. The mortality of eggs and adult mites were 85.83 and 63.07%, respectively. Moreover, fraction 2 showed moderate oviposition inhibition effect (0.8795) against P. citri when the used dose was higher than 2.5 mg mL^-1
基金Supported by Scientific and Technological Project of Chongqing City(CSTC,2010AC1018)Development Plan for Changjiang Scholars and Innovative Team(IRT0976)+1 种基金Special Fund for Agro-scientific Research in the Public Interest(200903032)Open Fund Projects of Municipal Key Laboratory for Citrus in Chongqing City
文摘Neoseiulus barker/Hughes is one of the best biological control products. The paper reviewed taxonomic status, biological characteristics, large-scale breeding and application research status of N. barkeri, so as to provide scientific basis for further research and application of N. barkeri.