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基于恒温无蛋白酶核酸扩增反应的生物传感器研究进展
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作者 王思媛 杨慧然 +4 位作者 翁本锐 冉家兵 王慧敏 杨昌英 邓张双 《三峡大学学报(自然科学版)》 CAS 2022年第3期104-112,共9页
恒温无蛋白酶的核酸扩增反应具有操作简单、条件温和、反应效率高,以及不需要温度循环和蛋白酶参与的特点,可以实现对生物分子的高灵敏检测,已经被广泛应用于生物分析和生物医学应用等领域.本文总结了近年来基于恒温无蛋白酶的核酸扩增... 恒温无蛋白酶的核酸扩增反应具有操作简单、条件温和、反应效率高,以及不需要温度循环和蛋白酶参与的特点,可以实现对生物分子的高灵敏检测,已经被广泛应用于生物分析和生物医学应用等领域.本文总结了近年来基于恒温无蛋白酶的核酸扩增反应的生物传感器在检测领域中的发展和应用,并对当前存在的问题进行了讨论. 展开更多
关键词 核酸扩增反应 熵驱动反应 杂交链式反应 催化发卡自组装反应 核酸酶
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A Novel GEL-OHA/HAp Bone Substitute
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作者 ran jiabing HU Jingxiao +2 位作者 CHEN Si JIANG Pei SHEN Xinyu 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2016年第6期491-498,共8页
Self-crosslinked gelatin-oxidized hyaluronic acid/hydroxyapatite(GEL-OHA/HAp) composite bone substitute was successfully prepared with a novel in situ precipitation method without using any toxic chemical cross-link... Self-crosslinked gelatin-oxidized hyaluronic acid/hydroxyapatite(GEL-OHA/HAp) composite bone substitute was successfully prepared with a novel in situ precipitation method without using any toxic chemical cross-linkers,and its characterizations,including elemental composition,surface morphology,crystallinity,and structure of crystalline phase,were carried out. In order to evaluate its corresponding performances,comparisons with glutaraldehyde crosslinked gelatin/hydroxyapatite(GEL-Glu/HAp) composite were made in detail. The results indicate that nano-HAp crystallites are homogeneously dispersed in both GEL-OHA/HAp and GEL-Glu/HAp composites,and the HAp crystallites in the former take larger particle size than those in the latter. Mechanical property tests show the acceptable mechanical strength at high strain of GEL-OHA/HAp composite. Study of in vitro degradation and swelling demonstrate that the two composites have similar degradation rate and water absorption capability.By in vitro cell culture,it has been found out that the cells on the GEL-OHA/HAp composite show higher proliferative potential than the cells on the GEL-Glu/HAp composite. Compared with GEL-Glu/HAp composite,GEL-OHA/HAp composite provides an excellent strategy for preparation of non-toxic bone substitute with acceptable corresponding properties. 展开更多
关键词 gelatin self-crosslinked oxidized hyaluronic acid
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