The Manchurian walnut(Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently. The antifatigue, antioxidative and immunoregulatory effects of Manchurian walnut hydrolysate ...The Manchurian walnut(Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently. The antifatigue, antioxidative and immunoregulatory effects of Manchurian walnut hydrolysate peptides(MWHPs)were evaluated in this study. MWHPs with a degree of hydrolysis of 32.23% were ultrafiltered and divided into three fractions,namely, high(> 10 k Da), medium(3–10 kDa), and low molecular weight(< 3 kDa), and then fed to mice continuously at doses of 200, 400 or 800 mg/(kg·d). The antifatigue, antioxidative, and immunoregulatory effects of the peptides were tested on the second and fourth weeks of MWHP administration. Results showed that low-molecular-weight MWHPs exerted significant antifatigue(prolonging swimming time, elevating liver glycogen contents, and reducing lactic acid contents), antioxidative(enhancing superoxide dismutase(SOD), GSH-Px, and catalase(CAT) activities and reducing malondialdehyde(MDA) content), and immunoregulatory(raising the immune-organ index and promoting T-lymphocyte proliferation and s Ig A secretion in the intestinal tract) effects. This research indicates that MWHPs have potential applications in health care and may be developed as a base for new functional foods.展开更多
从辣白菜中挑出105株菌株,革兰氏染色后筛选出18株杆菌,其余为球菌。经耐受性试验初步筛选8株疑似乳酸杆菌,做后续功能特性研究,包括菌株黏附性、抗氧化、降亚硝酸盐、同化胆固醇等。结果表明,A11,A16具有很强的胃酸耐受性(存活率大于75...从辣白菜中挑出105株菌株,革兰氏染色后筛选出18株杆菌,其余为球菌。经耐受性试验初步筛选8株疑似乳酸杆菌,做后续功能特性研究,包括菌株黏附性、抗氧化、降亚硝酸盐、同化胆固醇等。结果表明,A11,A16具有很强的胃酸耐受性(存活率大于75%);A15,A16耐胆盐能力较强(存活率大于64%)。A15对蛋白黏着力较强,疏水性较高。体外功能性试验表明,A16抗氧化性能较高,A1降亚硝酸钠能力强(清除率大于75%),A16胆固醇清除能力远优于其它,清除率大于57%。综上所述,A1,A11,A15,A16是相对优势菌株,益生功能较强,经16S r RNA的PCR产物鉴定A1,A11,A15是植物乳杆菌,A16是唾液乳杆菌。展开更多
This study assessed and compared the immunogenicity of various immunization strategies in mice using combinations of re- combinant DNA （pCCMp24） and recombinant attenuated vaccinia virus Tian Tan （rddVTT_ccMpe4）. ...This study assessed and compared the immunogenicity of various immunization strategies in mice using combinations of re- combinant DNA （pCCMp24） and recombinant attenuated vaccinia virus Tian Tan （rddVTT_ccMpe4）. Intramuscular immuniza- tion was performed on days 0 （prime） and 21 （boost）. The immunogenicity of the vaccine schedules was determined by meas- uring human immunodeficiency virus （HIV）-specific binding antibody levels and cytokine （interleukin-2 and interleukin-4） concentrations in peripheral blood, analyzing lymphocyte proliferation capacity against HIV epitopes and CD4~/CD8＋cell ratio, and monitoring interferon-gamma levels at different times post-immunization. The results showed that pCCMp24, rddVTT.ccMp24 and their prime-boost immunization induced humoral and cellular immune responses. The pCCMp24/ rddVTT.ccMp24 immunization strategy increased CD8＋ T cells and induced more IFN-7-secreting cells compared with sin- gle-shot rDNA. The prime-boost immunization strategy also induced the generation of cellular immunological memory to HIV epitope peptides. These results demonstrated that prime-boost immunization with rDNA and rddVTT_ccMp24 had a tendency to induce greater cellular immune response than single-shot vaccinations, especially IFN-7 response, providing a basis for further studies.展开更多
基金Supported by National High Technology Research and Development Program(No.2013AA102206-2)
文摘The Manchurian walnut(Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently. The antifatigue, antioxidative and immunoregulatory effects of Manchurian walnut hydrolysate peptides(MWHPs)were evaluated in this study. MWHPs with a degree of hydrolysis of 32.23% were ultrafiltered and divided into three fractions,namely, high(> 10 k Da), medium(3–10 kDa), and low molecular weight(< 3 kDa), and then fed to mice continuously at doses of 200, 400 or 800 mg/(kg·d). The antifatigue, antioxidative, and immunoregulatory effects of the peptides were tested on the second and fourth weeks of MWHP administration. Results showed that low-molecular-weight MWHPs exerted significant antifatigue(prolonging swimming time, elevating liver glycogen contents, and reducing lactic acid contents), antioxidative(enhancing superoxide dismutase(SOD), GSH-Px, and catalase(CAT) activities and reducing malondialdehyde(MDA) content), and immunoregulatory(raising the immune-organ index and promoting T-lymphocyte proliferation and s Ig A secretion in the intestinal tract) effects. This research indicates that MWHPs have potential applications in health care and may be developed as a base for new functional foods.
文摘从辣白菜中挑出105株菌株,革兰氏染色后筛选出18株杆菌,其余为球菌。经耐受性试验初步筛选8株疑似乳酸杆菌,做后续功能特性研究,包括菌株黏附性、抗氧化、降亚硝酸盐、同化胆固醇等。结果表明,A11,A16具有很强的胃酸耐受性(存活率大于75%);A15,A16耐胆盐能力较强(存活率大于64%)。A15对蛋白黏着力较强,疏水性较高。体外功能性试验表明,A16抗氧化性能较高,A1降亚硝酸钠能力强(清除率大于75%),A16胆固醇清除能力远优于其它,清除率大于57%。综上所述,A1,A11,A15,A16是相对优势菌株,益生功能较强,经16S r RNA的PCR产物鉴定A1,A11,A15是植物乳杆菌,A16是唾液乳杆菌。
基金supported by the National Natural Science Foundation of China (81001342)the National Basic Research Program of China (2011CB512110)the National Mega Project on Major Infectious Diseases Prevention (2012ZX10001005-006)
文摘This study assessed and compared the immunogenicity of various immunization strategies in mice using combinations of re- combinant DNA （pCCMp24） and recombinant attenuated vaccinia virus Tian Tan （rddVTT_ccMpe4）. Intramuscular immuniza- tion was performed on days 0 （prime） and 21 （boost）. The immunogenicity of the vaccine schedules was determined by meas- uring human immunodeficiency virus （HIV）-specific binding antibody levels and cytokine （interleukin-2 and interleukin-4） concentrations in peripheral blood, analyzing lymphocyte proliferation capacity against HIV epitopes and CD4~/CD8＋cell ratio, and monitoring interferon-gamma levels at different times post-immunization. The results showed that pCCMp24, rddVTT.ccMp24 and their prime-boost immunization induced humoral and cellular immune responses. The pCCMp24/ rddVTT.ccMp24 immunization strategy increased CD8＋ T cells and induced more IFN-7-secreting cells compared with sin- gle-shot rDNA. The prime-boost immunization strategy also induced the generation of cellular immunological memory to HIV epitope peptides. These results demonstrated that prime-boost immunization with rDNA and rddVTT_ccMp24 had a tendency to induce greater cellular immune response than single-shot vaccinations, especially IFN-7 response, providing a basis for further studies.
