Based on Landsat TM images in 2005 and urban planning data of 2005-2020 in Hangzhou Bay Industrial Belt, this paper studied the potential risk of the planned urban construction land extension to the wetlands by employ...Based on Landsat TM images in 2005 and urban planning data of 2005-2020 in Hangzhou Bay Industrial Belt, this paper studied the potential risk of the planned urban construction land extension to the wetlands by employing a GIS spatial analysis method. The results show that： （1） The wetland resources are widely distributed and diverse, with a proximal distribution to present urban construction lands spatially. （2） From 2005 to 2020, the urban construction lands will expand vastly and rapidly, and will take over large areas of wetlands that are mostly the ponds and the aquiculture water areas in the reclamation areas of Hangzhou Bay south coast. Moreover, this change will be spatially uneven obviously, e.g., the most serious recessions of the wetlands are concentrated in Ningbo city, Hangzhou city and Cixi city. （3） Overall, the potential risk to wetlands is very high, and will keep increasing with the trend of urbanization. （4） The quantity and spatial pattern of the planning urban construction land have substantial impacts on the wetland loss. Therefore, some policies and regulations are suggested to coordinate the relationship between urban economic development and wetland resource protection such as adjusting the construction land planning, compensating for wetland occupation as well as constructing wetland parks.展开更多
Credible evidence shows that odorant binding proteins(OBPs)are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors(OR...Credible evidence shows that odorant binding proteins(OBPs)are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors(ORs).In the present study,a novel OBP(AlinOBP3)gene from the lucerne plant bug,Adelphocoris lineolatus,was cloned and expressed.The expression pattern of AlinOBP3 was evaluated by qPCR,which indicated that AlinOBP3 was dominantly expressed in antennae.The binding properties of AlinOBP3 with 9 cotton volatiles and 5 sex pheromone analogs were measured by fluorescence competitive binding assays with the fluorescence probe 1-NPN.The results revealed that of 9 cotton volatiles,Myrcene,β-Ocimene and α-Phellandrene can bind with AlinOBP3.α-Phellandrene especially bound to AlinOBP3 with a high binding affinity,with a dissociation constant of 56.68 μmol/L.Of the 5 sex pheromone analogs,Hexyl butyrate had the strongest binding affinity with AlinOBP3,with a dissociation constant as 59.53 μmol/L.Butyl butyrate,trans-2-Hexenyl butyrate and Ethyl butyrate had medium binding affinities with AlinOBP3,with dissociation constants of 227.39,108.77 and 143.47 μmol/L,respectively.The results suggest that AlinOBP3 might be a pheromone binding protein(PBP)with a dual-function for the perception of sex pheromones and plant volatiles.展开更多
基金Key Project of Zhejiang Provincial Science and Technology Programs, No.2005C23059 National Key Technology R＆D Program of China, No.2006BAJ11B03 National Natural Science Foundation of China, No.40701177 The authors thank Prof. Liu Xinguo from Zhejiang University for his modification of language.
文摘Based on Landsat TM images in 2005 and urban planning data of 2005-2020 in Hangzhou Bay Industrial Belt, this paper studied the potential risk of the planned urban construction land extension to the wetlands by employing a GIS spatial analysis method. The results show that： （1） The wetland resources are widely distributed and diverse, with a proximal distribution to present urban construction lands spatially. （2） From 2005 to 2020, the urban construction lands will expand vastly and rapidly, and will take over large areas of wetlands that are mostly the ponds and the aquiculture water areas in the reclamation areas of Hangzhou Bay south coast. Moreover, this change will be spatially uneven obviously, e.g., the most serious recessions of the wetlands are concentrated in Ningbo city, Hangzhou city and Cixi city. （3） Overall, the potential risk to wetlands is very high, and will keep increasing with the trend of urbanization. （4） The quantity and spatial pattern of the planning urban construction land have substantial impacts on the wetland loss. Therefore, some policies and regulations are suggested to coordinate the relationship between urban economic development and wetland resource protection such as adjusting the construction land planning, compensating for wetland occupation as well as constructing wetland parks.
基金supported by the National Key Basic Research Program of China(2007CB109202)the National High-Technology Research and Development Program of China(2008BAD5B05)National Natural Science Foundation of China(31071694)
文摘Credible evidence shows that odorant binding proteins(OBPs)are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors(ORs).In the present study,a novel OBP(AlinOBP3)gene from the lucerne plant bug,Adelphocoris lineolatus,was cloned and expressed.The expression pattern of AlinOBP3 was evaluated by qPCR,which indicated that AlinOBP3 was dominantly expressed in antennae.The binding properties of AlinOBP3 with 9 cotton volatiles and 5 sex pheromone analogs were measured by fluorescence competitive binding assays with the fluorescence probe 1-NPN.The results revealed that of 9 cotton volatiles,Myrcene,β-Ocimene and α-Phellandrene can bind with AlinOBP3.α-Phellandrene especially bound to AlinOBP3 with a high binding affinity,with a dissociation constant of 56.68 μmol/L.Of the 5 sex pheromone analogs,Hexyl butyrate had the strongest binding affinity with AlinOBP3,with a dissociation constant as 59.53 μmol/L.Butyl butyrate,trans-2-Hexenyl butyrate and Ethyl butyrate had medium binding affinities with AlinOBP3,with dissociation constants of 227.39,108.77 and 143.47 μmol/L,respectively.The results suggest that AlinOBP3 might be a pheromone binding protein(PBP)with a dual-function for the perception of sex pheromones and plant volatiles.
