Spinal anesthesia is a common technique which can significantly alleviate surgery patients' anguish, and can avoid the risks associated with general anesthesia. However, spinal anesthesia is associated with some o...Spinal anesthesia is a common technique which can significantly alleviate surgery patients' anguish, and can avoid the risks associated with general anesthesia. However, spinal anesthesia is associated with some other risks. The most common adverse event is hypotension resulting from a near complete sympathetic block. Nearly 1/3 of non-obstetric patients experience spinal anesthesia-induced hypotension. Moreover, in obstetric patients without pharmacological prophylaxis, the incidence rate of spinal anesthesia-induced hypotension can reach up to 70%-80%[1].展开更多
Objective To explore the protective effects of dexmedetomidine(Dex)against high glucose-induced epithelial-mesenchymal transition in HK-2 cells and relevant mechanisms.Methods HK-2 cells were exposed to either glucose...Objective To explore the protective effects of dexmedetomidine(Dex)against high glucose-induced epithelial-mesenchymal transition in HK-2 cells and relevant mechanisms.Methods HK-2 cells were exposed to either glucose or glucose+Dex for 6 h.The production of ROS,morphology of HK-2 cells,and cell cycle were detected.Moreover,the expression of AKT,p-AKT,ERK,pERK,PI3 K,E-Cadherin,Claudin-1,andα-SMA were determined and compared between HK-2 cells exposed to glucose and those exposed to both glucose and Dex with or without PI3 K/AKT pathway inhibitor LY294002 and ERK pathway inhibitor U0126.Results Compared with HK-2 cells exposed to high level of glucose,the HK-2 cells exposed to both high level of glucose and Dex showed:(1)lower level of ROS production;(2)cell morphology was complete;(3)more cells in G1 phase;(4)lower expression of p-AKT,p-ERK andα-SMA,higher expression of ECadherin and Claudin-1.PI3 K/AKT inhibitor LY294002 and ERK inhibitor U0126 decreased the expression of p-AKT,p-ERK andα-SMA,and increased the expression of E-Cadherin and Claudin-1.Conclusion Dex can attenuate high glucose-induced HK-2 epithelial-mesenchymal transition by inhibiting AKT and ERK.展开更多
Objective To identify the important risk factors for type 2 Diabetes Mellitus(T2 DM) and develop effective strategies to address the problem of T2 DM. Our study aimed to evaluate the association between apolipoprotein...Objective To identify the important risk factors for type 2 Diabetes Mellitus(T2 DM) and develop effective strategies to address the problem of T2 DM. Our study aimed to evaluate the association between apolipoprotein E(Apo E) genetic polymorphism and type 2 diabetes, and to provide clues for the etiology of T2 DM.Methods Based on the criteria of inclusion and exclusion, we extracted, pooled, analyzed and assessed the case-control studies of Apo E polymorphism and T2 DM published in Pub Med, Web of Science,Medline, Wan Fang, VIP, and CNKI databases by R soft-ware(version 3.4.3). We used Random-effect models when heterogeneity was present in between-study, and fixed-effect models otherwise.Results We had 59 studies covering 6,872 cases with T2 DM and 8,250 controls, and compared the alleles and genotypes of Apo E between cases and controls. When we conducted a comparison between Apo E ε4 and ε3 alleles, we produced a pooled OR of 1.18(95% CI: 1.09-1.28;P < 0.001). Apo E ε2/ε2 genotype displayed a possible association with T2 DM(OR = 1.46;95% CI: 1.11-1.93;P = 0.007), ε3/ε4 genotype showed a 1.11-fold risk(OR = 1.11;95% CI: 1.01-1.22;P = 0.039) and ε4/ε4 genotype had a1.71-fold risk of developing T2 DM(OR = 1.71;95% CI: 1.33-2.19;P < 0.001) when they were compared with ε3/ε3 genotype.Conclusions There is an association between Apo E polymorphism and T2 DM: allele ε4 and genotypes(ε2/ε2, ε3/ε4, and ε4/ε4) are associated with the increased risk for the development of T2 DM, and they may be risk factors for T2 DM.展开更多
A case-control study was conducted to investigate associations between organophosphate pesticide（OP）exposure,aggression,impulsivity,and attempted suicide.Questionnaires were used to collect information;impulsivity a...A case-control study was conducted to investigate associations between organophosphate pesticide（OP）exposure,aggression,impulsivity,and attempted suicide.Questionnaires were used to collect information;impulsivity and aggression were measured by the Barratt Impulsivity Scale（BIS）and the Aggression Inventory（AI）.展开更多
Objective Vitamin D and Toll-like receptor-4(TLR-4)inhibition are involved in the protection of keratinocytes.The effects of combination of 1,25(OH)_(2)D_(3) and TLR-4 inhibitor on the protection of keratinocytes agai...Objective Vitamin D and Toll-like receptor-4(TLR-4)inhibition are involved in the protection of keratinocytes.The effects of combination of 1,25(OH)_(2)D_(3) and TLR-4 inhibitor on the protection of keratinocytes against ultraviolet radiation B(UVB)irradiation remain unclear.This study was undertaken to explore the effects of combination of 1,25(OH)_(2)D_(3) and TAK-242(TLR-4 inhibitor)on the damage to HaCaT cells caused by UVB irradiation.Methods In vitro,HaCaT cells were treated with 1,25(OH)_(2)D_(3) or/and TAK-242 prior to UVB irradiation at the intensity of 20 mJ/cm^(2),then the production of reactive oxygen species(ROS),cell migration,apoptosis of cells,and the expression of oxidative stress,endoplasmic reticulum stress,and apoptosis related proteins were determined.Results Compared with the HaCaT cells treated with 1,25(OH)_(2)D_(3) or TAK-242,the cells treated with both 1,25(OH)_(2)D_(3) and TAK-242 showed,1)significantly lower production of ROS(P<0.05);2)significantly less apoptosis of HaCaT cells(P<0.05);3)significantly lower expression of NF-κB,Caspase-8,Cyto-C,Caspase-3(P<0.05).Conclusion The combination of 1,25(OH)_(2)D_(3) and TAK-242 could produce a better protection for HaCaT cells via inhibiting the oxidative stress,endoplasmic reticulum stress and apoptosis than 1,25(OH)_(2)D_(3) or TAK-242 alone.展开更多
According to the report of the International Diabetes Federation, there were 382 million people affected with diabetes in 2013 and it is expected that this number will increase to 592 million by the year 2035;. Diabet...According to the report of the International Diabetes Federation, there were 382 million people affected with diabetes in 2013 and it is expected that this number will increase to 592 million by the year 2035;. Diabetes is caused due to the interaction between environmental and genetic factors;.展开更多
Globally,breast cancer is the second major cause of cancer death among women[1].Cyclophosphamide(CTX)is a common chemotherapeutic agent for patients with breast cancer who have undergone surgery.CTX metabolites act by...Globally,breast cancer is the second major cause of cancer death among women[1].Cyclophosphamide(CTX)is a common chemotherapeutic agent for patients with breast cancer who have undergone surgery.CTX metabolites act by disrupting deoxyribonucleic acid and by arresting the cell cycle of tumor cells,which results in their apoptosis or growth inhibition[2].However,CTX triggers the production of excessive amounts of reactive oxygen species(ROS),which produces various side effects in different organs or systems[3].In the initial stage of cancer development,ROS may promote the expansion of cancerous cells by modifying apoptosis-related genes[4].Furthermore,ROS will activate the NF-κB pathway and NF-κB-dependent genes that are involved in apoptosis and cell adhesion,which are implicated in multiple aspects of cancer development[5].ROS,as a survival or apoptotic signal to cancer cells,is determined by the dosage,duration,type,and site of ROS production[6].展开更多
Organophosphate(OP)pesticides are a class of chemicals that are typically derived from phosphoric,phosphonic,and phosphinic acids,and are commonly used in agriculture[1].China is the largest producer of pesticides and...Organophosphate(OP)pesticides are a class of chemicals that are typically derived from phosphoric,phosphonic,and phosphinic acids,and are commonly used in agriculture[1].China is the largest producer of pesticides and one of the most intensive pesticide users in crop production in the world.Pesticide poisoning has been shown to be an important public health problem among Chinese farm workers[2].Multiple routes of exposure to OP pesticides occur through food contamination,environmental and household pollution,proximity to agricultural fields,and agricultural work[3].展开更多
Ultraviolet radiation(UVR)is a public health concern due to the depletion in ozone layer and the excessive exposure to UVR has been recognized as one of the major environmental risk factors for various skin diseases i...Ultraviolet radiation(UVR)is a public health concern due to the depletion in ozone layer and the excessive exposure to UVR has been recognized as one of the major environmental risk factors for various skin diseases including the damage on keratinocyte which leading to formation of cutaneous malignancies[1].UVR consists of UVA(320-400nm),UVB(290-320nm)and UVC(200-290nm)[2].展开更多
文摘Spinal anesthesia is a common technique which can significantly alleviate surgery patients' anguish, and can avoid the risks associated with general anesthesia. However, spinal anesthesia is associated with some other risks. The most common adverse event is hypotension resulting from a near complete sympathetic block. Nearly 1/3 of non-obstetric patients experience spinal anesthesia-induced hypotension. Moreover, in obstetric patients without pharmacological prophylaxis, the incidence rate of spinal anesthesia-induced hypotension can reach up to 70%-80%[1].
文摘Objective To explore the protective effects of dexmedetomidine(Dex)against high glucose-induced epithelial-mesenchymal transition in HK-2 cells and relevant mechanisms.Methods HK-2 cells were exposed to either glucose or glucose+Dex for 6 h.The production of ROS,morphology of HK-2 cells,and cell cycle were detected.Moreover,the expression of AKT,p-AKT,ERK,pERK,PI3 K,E-Cadherin,Claudin-1,andα-SMA were determined and compared between HK-2 cells exposed to glucose and those exposed to both glucose and Dex with or without PI3 K/AKT pathway inhibitor LY294002 and ERK pathway inhibitor U0126.Results Compared with HK-2 cells exposed to high level of glucose,the HK-2 cells exposed to both high level of glucose and Dex showed:(1)lower level of ROS production;(2)cell morphology was complete;(3)more cells in G1 phase;(4)lower expression of p-AKT,p-ERK andα-SMA,higher expression of ECadherin and Claudin-1.PI3 K/AKT inhibitor LY294002 and ERK inhibitor U0126 decreased the expression of p-AKT,p-ERK andα-SMA,and increased the expression of E-Cadherin and Claudin-1.Conclusion Dex can attenuate high glucose-induced HK-2 epithelial-mesenchymal transition by inhibiting AKT and ERK.
基金supported by the Jipa Ruida Environmental Inspection Corporation Limited,Beijing under Grant Radioactive Diagnosis and Treatment Construction Project-Radiation Protection and Evaluation [Grant No.2016YX137]Jilin Province Pharmacy Operation Corporation,Limited [Grant No.371182093427]
文摘Objective To identify the important risk factors for type 2 Diabetes Mellitus(T2 DM) and develop effective strategies to address the problem of T2 DM. Our study aimed to evaluate the association between apolipoprotein E(Apo E) genetic polymorphism and type 2 diabetes, and to provide clues for the etiology of T2 DM.Methods Based on the criteria of inclusion and exclusion, we extracted, pooled, analyzed and assessed the case-control studies of Apo E polymorphism and T2 DM published in Pub Med, Web of Science,Medline, Wan Fang, VIP, and CNKI databases by R soft-ware(version 3.4.3). We used Random-effect models when heterogeneity was present in between-study, and fixed-effect models otherwise.Results We had 59 studies covering 6,872 cases with T2 DM and 8,250 controls, and compared the alleles and genotypes of Apo E between cases and controls. When we conducted a comparison between Apo E ε4 and ε3 alleles, we produced a pooled OR of 1.18(95% CI: 1.09-1.28;P < 0.001). Apo E ε2/ε2 genotype displayed a possible association with T2 DM(OR = 1.46;95% CI: 1.11-1.93;P = 0.007), ε3/ε4 genotype showed a 1.11-fold risk(OR = 1.11;95% CI: 1.01-1.22;P = 0.039) and ε4/ε4 genotype had a1.71-fold risk of developing T2 DM(OR = 1.71;95% CI: 1.33-2.19;P < 0.001) when they were compared with ε3/ε3 genotype.Conclusions There is an association between Apo E polymorphism and T2 DM: allele ε4 and genotypes(ε2/ε2, ε3/ε4, and ε4/ε4) are associated with the increased risk for the development of T2 DM, and they may be risk factors for T2 DM.
基金partially supported by the Colorado Injury Control Research Center Grant Number 5R49CE001168 from the Centers for Disease Control and Preventionthe USA-China Agricultural Injury Research Training Project Grant Number 5DW43TW007257 from the National Institutes of Health,Fogarty International Center
文摘A case-control study was conducted to investigate associations between organophosphate pesticide（OP）exposure,aggression,impulsivity,and attempted suicide.Questionnaires were used to collect information;impulsivity and aggression were measured by the Barratt Impulsivity Scale（BIS）and the Aggression Inventory（AI）.
基金supported by the Department of Science and Technology of Jilin Province.[Grant No:20210204024YY]。
文摘Objective Vitamin D and Toll-like receptor-4(TLR-4)inhibition are involved in the protection of keratinocytes.The effects of combination of 1,25(OH)_(2)D_(3) and TLR-4 inhibitor on the protection of keratinocytes against ultraviolet radiation B(UVB)irradiation remain unclear.This study was undertaken to explore the effects of combination of 1,25(OH)_(2)D_(3) and TAK-242(TLR-4 inhibitor)on the damage to HaCaT cells caused by UVB irradiation.Methods In vitro,HaCaT cells were treated with 1,25(OH)_(2)D_(3) or/and TAK-242 prior to UVB irradiation at the intensity of 20 mJ/cm^(2),then the production of reactive oxygen species(ROS),cell migration,apoptosis of cells,and the expression of oxidative stress,endoplasmic reticulum stress,and apoptosis related proteins were determined.Results Compared with the HaCaT cells treated with 1,25(OH)_(2)D_(3) or TAK-242,the cells treated with both 1,25(OH)_(2)D_(3) and TAK-242 showed,1)significantly lower production of ROS(P<0.05);2)significantly less apoptosis of HaCaT cells(P<0.05);3)significantly lower expression of NF-κB,Caspase-8,Cyto-C,Caspase-3(P<0.05).Conclusion The combination of 1,25(OH)_(2)D_(3) and TAK-242 could produce a better protection for HaCaT cells via inhibiting the oxidative stress,endoplasmic reticulum stress and apoptosis than 1,25(OH)_(2)D_(3) or TAK-242 alone.
基金partially supported by Jipai Runda Environmental Inspection Technology Corporation Limited of Beijing [Grant No.2016YX137]the grant of research on management model of common chronic diseases [Grant No.371182093427]
文摘According to the report of the International Diabetes Federation, there were 382 million people affected with diabetes in 2013 and it is expected that this number will increase to 592 million by the year 2035;. Diabetes is caused due to the interaction between environmental and genetic factors;.
基金supported by Leshiguang Corp. Limited [2018YX046]。
文摘Globally,breast cancer is the second major cause of cancer death among women[1].Cyclophosphamide(CTX)is a common chemotherapeutic agent for patients with breast cancer who have undergone surgery.CTX metabolites act by disrupting deoxyribonucleic acid and by arresting the cell cycle of tumor cells,which results in their apoptosis or growth inhibition[2].However,CTX triggers the production of excessive amounts of reactive oxygen species(ROS),which produces various side effects in different organs or systems[3].In the initial stage of cancer development,ROS may promote the expansion of cancerous cells by modifying apoptosis-related genes[4].Furthermore,ROS will activate the NF-κB pathway and NF-κB-dependent genes that are involved in apoptosis and cell adhesion,which are implicated in multiple aspects of cancer development[5].ROS,as a survival or apoptotic signal to cancer cells,is determined by the dosage,duration,type,and site of ROS production[6].
基金supported by the USA-China Agricultural Injury Research Training Project Grant Number 5DW43TW007257 from the National Institutes of Health,Fogarty International Center.
文摘Organophosphate(OP)pesticides are a class of chemicals that are typically derived from phosphoric,phosphonic,and phosphinic acids,and are commonly used in agriculture[1].China is the largest producer of pesticides and one of the most intensive pesticide users in crop production in the world.Pesticide poisoning has been shown to be an important public health problem among Chinese farm workers[2].Multiple routes of exposure to OP pesticides occur through food contamination,environmental and household pollution,proximity to agricultural fields,and agricultural work[3].
基金supported by the Jipa Ruida Environmental Inspection Corporation Limited,Beijing under Grant Radioactive Diagnosis and Treatment Construction Project-Radiation Protection and Evaluation [number 2016YX137]PET/CT construction project-Radiation Protection Pre-evaluation [number 2015YX252]
文摘Ultraviolet radiation(UVR)is a public health concern due to the depletion in ozone layer and the excessive exposure to UVR has been recognized as one of the major environmental risk factors for various skin diseases including the damage on keratinocyte which leading to formation of cutaneous malignancies[1].UVR consists of UVA(320-400nm),UVB(290-320nm)and UVC(200-290nm)[2].
