Nutrient and stress factor 1(Nsf1),a transcription factor containing the classical Cys2-His2(C_(2)H_(2))zinc finger motif,is expressed under non-fermentable carbon conditions and in response to salt stress in Saccharo...Nutrient and stress factor 1(Nsf1),a transcription factor containing the classical Cys2-His2(C_(2)H_(2))zinc finger motif,is expressed under non-fermentable carbon conditions and in response to salt stress in Saccharomyces cerevisiae.However,the role of Nsf1 in filamentous fungi is not well understood.In this study,the orthologue of Nsf1 was investigated in Fusarium graminearum(named FgNsf1),a causal agent of Fusarium head blight(FHB).The functions of FgNsf1 were evaluated by constructing a FgNSF1 deletion mutant,designated asΔFgNsf1,and its functional complementation mutantΔFgNsf1-C.Gene deletion experiments showed that the mycelial growth rate,asexual and sexual reproduction ofΔFgNsf1 were significantly reduced,but the pigment production ofΔFgNsf1 was remarkably increased compared with the PH-1 andΔFgNsf1-C.In addition,the tolerance ofΔFgNsf1 to osmotic pressures,cell wall-damaging agents and oxidative stress increased significantly.Sensitivity tests to different fungicides revealed thatΔFgNsf1 exhibited increased sensitivity to carbendazim(MBC)and tebuconazole,and enhanced tolerance to fludioxonil and iprodione than PH-1 andΔFgNsf1-C.The virulence ofΔFgNsf1 to wheat coleoptiles and flowering wheat heads were dramatically decreased,which was consistent with the decrease in the yield of deoxynivalenol(DON).All of these defects were restored by target gene complementation.These results indicated that FgNsf1 plays a crucial role in vegetative growth,asexual and sexual reproduction,stress responses,fungicide sensitivity,and full virulence in F.graminearum.展开更多
Botryosphaeria dothidea is a destructive fungal pathogen that causes Botryosphaeria canker and fruit ring rot on apple worldwide.Autophagy is a process of self-degradation that maintains intracellular homeostasis via ...Botryosphaeria dothidea is a destructive fungal pathogen that causes Botryosphaeria canker and fruit ring rot on apple worldwide.Autophagy is a process of self-degradation that maintains intracellular homeostasis via lysosomal pathway.To date,the biological role of autophagy in B.dothidea remains unknown.In this study,we identified and characterized the autophagy-related gene BdATG8 in B.dothidea.BdATG8 was able to restore the defect in nitrogen starvation tolerance of Saccharomyces cerevisiae ATG8 deletion mutant.GFP-BdAtg8 was shown to be a useful marker for monitoring autophagy in B.dothidea.Target deletion of Bd ATG8(ΔBd Atg8)blocked autophagy and significantly impaired mycelial growth,conidiation and perithecium formation.In addition,ΔBdAtg8 showed significantly increased sensitivity to phytoalexin and oxidative stress,suggesting that BdATG8 plays critical roles in overcoming phytoalexin and reactive oxygen species(ROS)-mediated plant immunity.Pathogenicity assays revealed thatΔBdAtg8 almost lost ability to infect hosts.Overall,our results indicate that BdATG8 plays an important role in fungal development,stress responses and pathogenesis in B.dothidea.展开更多
Grapevine(Vitis vinifera L.)is an economically important fruit crop in the world,and China ranks first in the production of grapes with approximately 15%of the world’s total yield.However,diseases that cause the deat...Grapevine(Vitis vinifera L.)is an economically important fruit crop in the world,and China ranks first in the production of grapes with approximately 15%of the world’s total yield.However,diseases that cause the death of grapevine shoots pose a severe threat to the production of grapes.In this study,the fungus Neopestalotiopsis eucalypti was identified as a causal pathogen of grapevine shoot rot based on the morphology of conidia and a phylogenetic analysis.The phylogenetic analysis was performed with three isolates based on the combined sequence of internal transcribed spacer(ITS)region of ribosomal DNA,part of the translation elongation factor 1-alpha(Tef)and theβ-tubulin(Tub2)genes.The three isolates were all identified as N.eucalypti.Pathogenicity tests of the three fungal isolates were conducted on grapevines shoots in vitro and in vivo.The results showed that all three fungal isolates caused severe rot lesions on the inoculated grapevine shoots,and N.eucalypti was re-isolated from the inoculated grapevine shoots.Therefore,N.eucalypti was confirmed as a causal agent of the grapevine shoot rot.This is the first report of N.eucalypti causing grapevine shoot disease in China.展开更多
基金This work was supported by the National Key Research&Development Program of China(2016YED0201007,2018YFD0201201 and 2018YFD0201000)the National Natural Science Foundation of China(31672065)+1 种基金the Agricultural Science and Technology Projects of Jiangsu Province,China(BE2018378,BA2018039,PZCZ201715,CX(19)3003,and CX(18)2005)the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX18_0670).
文摘Nutrient and stress factor 1(Nsf1),a transcription factor containing the classical Cys2-His2(C_(2)H_(2))zinc finger motif,is expressed under non-fermentable carbon conditions and in response to salt stress in Saccharomyces cerevisiae.However,the role of Nsf1 in filamentous fungi is not well understood.In this study,the orthologue of Nsf1 was investigated in Fusarium graminearum(named FgNsf1),a causal agent of Fusarium head blight(FHB).The functions of FgNsf1 were evaluated by constructing a FgNSF1 deletion mutant,designated asΔFgNsf1,and its functional complementation mutantΔFgNsf1-C.Gene deletion experiments showed that the mycelial growth rate,asexual and sexual reproduction ofΔFgNsf1 were significantly reduced,but the pigment production ofΔFgNsf1 was remarkably increased compared with the PH-1 andΔFgNsf1-C.In addition,the tolerance ofΔFgNsf1 to osmotic pressures,cell wall-damaging agents and oxidative stress increased significantly.Sensitivity tests to different fungicides revealed thatΔFgNsf1 exhibited increased sensitivity to carbendazim(MBC)and tebuconazole,and enhanced tolerance to fludioxonil and iprodione than PH-1 andΔFgNsf1-C.The virulence ofΔFgNsf1 to wheat coleoptiles and flowering wheat heads were dramatically decreased,which was consistent with the decrease in the yield of deoxynivalenol(DON).All of these defects were restored by target gene complementation.These results indicated that FgNsf1 plays a crucial role in vegetative growth,asexual and sexual reproduction,stress responses,fungicide sensitivity,and full virulence in F.graminearum.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-27)the National Natural Science Foundation of China(32001937)the Scientific Research Fund for High-level Talents in Qingdao Agricultural University,China(665/1120060)。
文摘Botryosphaeria dothidea is a destructive fungal pathogen that causes Botryosphaeria canker and fruit ring rot on apple worldwide.Autophagy is a process of self-degradation that maintains intracellular homeostasis via lysosomal pathway.To date,the biological role of autophagy in B.dothidea remains unknown.In this study,we identified and characterized the autophagy-related gene BdATG8 in B.dothidea.BdATG8 was able to restore the defect in nitrogen starvation tolerance of Saccharomyces cerevisiae ATG8 deletion mutant.GFP-BdAtg8 was shown to be a useful marker for monitoring autophagy in B.dothidea.Target deletion of Bd ATG8(ΔBd Atg8)blocked autophagy and significantly impaired mycelial growth,conidiation and perithecium formation.In addition,ΔBdAtg8 showed significantly increased sensitivity to phytoalexin and oxidative stress,suggesting that BdATG8 plays critical roles in overcoming phytoalexin and reactive oxygen species(ROS)-mediated plant immunity.Pathogenicity assays revealed thatΔBdAtg8 almost lost ability to infect hosts.Overall,our results indicate that BdATG8 plays an important role in fungal development,stress responses and pathogenesis in B.dothidea.
基金the financial support from the earmarked fund for China Agriculture Research System(CARS-27)
文摘Grapevine(Vitis vinifera L.)is an economically important fruit crop in the world,and China ranks first in the production of grapes with approximately 15%of the world’s total yield.However,diseases that cause the death of grapevine shoots pose a severe threat to the production of grapes.In this study,the fungus Neopestalotiopsis eucalypti was identified as a causal pathogen of grapevine shoot rot based on the morphology of conidia and a phylogenetic analysis.The phylogenetic analysis was performed with three isolates based on the combined sequence of internal transcribed spacer(ITS)region of ribosomal DNA,part of the translation elongation factor 1-alpha(Tef)and theβ-tubulin(Tub2)genes.The three isolates were all identified as N.eucalypti.Pathogenicity tests of the three fungal isolates were conducted on grapevines shoots in vitro and in vivo.The results showed that all three fungal isolates caused severe rot lesions on the inoculated grapevine shoots,and N.eucalypti was re-isolated from the inoculated grapevine shoots.Therefore,N.eucalypti was confirmed as a causal agent of the grapevine shoot rot.This is the first report of N.eucalypti causing grapevine shoot disease in China.
