Objective:Electroacupuncture(EA)is good at post-surgical pain.But point selection method in treating incision-induced pain remains a major clinical challenge.We reasoned that different acupoints may work though adjust...Objective:Electroacupuncture(EA)is good at post-surgical pain.But point selection method in treating incision-induced pain remains a major clinical challenge.We reasoned that different acupoints may work though adjusting prostaglandin E2 in spinal cord.We wish to explore the analgesic mechanism of electroacupuncture on plantar incision pain rats and provide more therapeutic ideas for acupuncture analgesia.Methods:A total of 50 male rats were randomly divided into a sham operation group,a model group,an EA 1,EA 2 and a drug group(n=10,each).A rat model of left plantar incision pain was established.The rats in EA1 group was needled at ipsilateral Yanglingquan(GB34)and Taixi(KI3).The rats in EA2 group was needled at ipsilateral Quchi(LI11)and Hegu(LI4).EA stimulation(2/100 Hz,1-2-3 mA)was administered 30 minutes immediately after operation.The rats in drug group were fed with Fenbid by gavage 20 minutes before incision(30 mg/kg,p.o.).The hot plate pain detector was used to measure the thermal pain threshold(TPT)before and 24 hours after operation Prostaglandin E2 content of spinal cord was detected by enzyme-linked immunosorbent assay(ELISA)at 1 and 24 hours after operation.Results:Compared with sham operation group,the TPT in model group decreased 41%.Compared with the model group,the TPT increased 56%in EA1,29%in EA2,190%in drug group(P>0.05).At 1 h after operation,compared with the sham operation group,PGE_2 in model group increased 15%.Compared with the model group,PGE_2 in drug group decreased 5%.At 24 hours after operation,compared with sham operation group,PGE_2 in model group increased 9%.Compared with model group,it decreased 4%in EA 1 group,8%in drug group and increased 3%in EA2 group.Conclusion:Both the drug and the electroacupuncture can adjust the 24-hour pain threshold and PGE_2 in spinal cord.The curative effects of the drug are better than that of electroacupuncture.The proximal point is better than that of the distal point.Electroacupuncture can treat postoperative pain by regulating PGE_2 in spinal cord.展开更多
Background The mechanism of acupuncture analgesia in craniotomy has been widely studied. However, the theoretical basis for selection of acupoints has not been examined. In this study, we used the regional homogeneit...Background The mechanism of acupuncture analgesia in craniotomy has been widely studied. However, the theoretical basis for selection of acupoints has not been examined. In this study, we used the regional homogeneity method blood oxygen level-dependent （BOLD） signals to determine changes in brain activity in response to transcutaneous electrical stimulation on acupoints and non-acupoints in resting state functional magnetic resonance imaging （fMRI）.Methods Twelve healthy volunteers were enrolled in this study. BOLD fMRI scanning of the brain was performed for 306 seconds before and 30 minutes after transcutaneous electrical stimulation on acupoints UB63 （Jinmen）, LV3 （Tai chong）, ST36 （Zusanli）, and GB40 （Qiuxu）. The procedure was repeated after one week with stimulation on non-acupoints （one was 9 above BL67, the second was 12 above BL67 （Kunlun）, the third was 7 above KI3, and the fourth was 10 above KI3 （Taixi））.Results The regional homogeneity in the acupoint group was increased in the left thalamus, caudate, putamen, lentiform nucleus （BA19, 30, 39）, postcentral gyrus, precentral gyrus （BA3, 4, 30, 32）, calcarine fissure, middle temporal gyrus （BA30）, right superior temporal gyrus, inferior temporal gyrus （BA38）, cuneus, and precuneus （BA7, 19） when compared to the non-acupoint group. The regional homogeneity of the acupoint group was decreased in the left cerebellum posterior lobe, middle frontal gyrus （BA10）, double-side precuneus （BA7）, and the postcentral gyrus （BA40）.Conclusions The brain region activated following acupoint stimulation is the ipsilateral pain-related brain region, which may relate to the therapeutic effect of acupuncture on pain relief. Further acupoint stimulation causes different central nervous responses compared to non-acupoint stimulation.展开更多
Objective： To investigate the effect of electroacupuncture（EA） treatment on the expression of cyclooxygenase（COX） 2 and microglia in spinal cord by using rat model of neuropathic pain, and to probe into the relat...Objective： To investigate the effect of electroacupuncture（EA） treatment on the expression of cyclooxygenase（COX） 2 and microglia in spinal cord by using rat model of neuropathic pain, and to probe into the relationship between COX 2 and microglia. Methods： The rats were randomly divided into 6 groups, including normal control group, model group, sham group, EA 1 group（distant acupoints ＋ local acupoints）, EA 2 group（local acupoints）, and EA 3 group（distant acupoints）. Thermal withdrawal latencies were evaluated at 1 day preoperatively and 3, 5 and 7 days postoperatively. At 7 days postoperatively, the spinal COX 2 m RNA was detected by reverse-transcription polymerase chain reaction. Double immunofluorescent staining technology was applied to screen and verify the relationship between altered COX 2 and microglia. Results： Compared with the model group, thermal withdrawal latencies increased after EA treatment（P〈0.01）. The expressions of COX 2 m RNA were up-regulated in spinal cord of rat on day 7 after surgery（P〈0.05）. Compared with the model group, EA stimulation（EA 1 and EA 2 groups） reversed the up-regulation of COX 2 m RNA expression（P〈0.05）. EA 1 and EA 2 groups might have better treatment effect compared with the EA 3 group. Fluorescent images displayed COX 2 and microglia expressed at common areas. Conclusions： EA was effective in analgesic and anti-inflammatory. EA has decreased the expression of spinal COX 2 m RNA in the trend of the therapeutic effect of ＂distant acupoints ＋ local acupoints＂, and ＂local acupoints＂ intervention may be superior to that of ＂distant acupoints＂ intervention. Microglia may be related to the formation of COX 2.展开更多
基金supported by a grant from National University Students'Innovation and Entrepreneurship Training project(No.201810026036)a grant from the National Key Basic Research and Development Program"973"Project(No.2007CB512503)。
文摘Objective:Electroacupuncture(EA)is good at post-surgical pain.But point selection method in treating incision-induced pain remains a major clinical challenge.We reasoned that different acupoints may work though adjusting prostaglandin E2 in spinal cord.We wish to explore the analgesic mechanism of electroacupuncture on plantar incision pain rats and provide more therapeutic ideas for acupuncture analgesia.Methods:A total of 50 male rats were randomly divided into a sham operation group,a model group,an EA 1,EA 2 and a drug group(n=10,each).A rat model of left plantar incision pain was established.The rats in EA1 group was needled at ipsilateral Yanglingquan(GB34)and Taixi(KI3).The rats in EA2 group was needled at ipsilateral Quchi(LI11)and Hegu(LI4).EA stimulation(2/100 Hz,1-2-3 mA)was administered 30 minutes immediately after operation.The rats in drug group were fed with Fenbid by gavage 20 minutes before incision(30 mg/kg,p.o.).The hot plate pain detector was used to measure the thermal pain threshold(TPT)before and 24 hours after operation Prostaglandin E2 content of spinal cord was detected by enzyme-linked immunosorbent assay(ELISA)at 1 and 24 hours after operation.Results:Compared with sham operation group,the TPT in model group decreased 41%.Compared with the model group,the TPT increased 56%in EA1,29%in EA2,190%in drug group(P>0.05).At 1 h after operation,compared with the sham operation group,PGE_2 in model group increased 15%.Compared with the model group,PGE_2 in drug group decreased 5%.At 24 hours after operation,compared with sham operation group,PGE_2 in model group increased 9%.Compared with model group,it decreased 4%in EA 1 group,8%in drug group and increased 3%in EA2 group.Conclusion:Both the drug and the electroacupuncture can adjust the 24-hour pain threshold and PGE_2 in spinal cord.The curative effects of the drug are better than that of electroacupuncture.The proximal point is better than that of the distal point.Electroacupuncture can treat postoperative pain by regulating PGE_2 in spinal cord.
基金This work was supported by the grants from the National Key Basic Research and Development Program ＂973＂ Project （No. 2007CB512503）, and the China Postdoctoral Science Foundation （No. 20070420403）.Acknowledgements： The authors are highly grateful to Professor ZANG Yu-feng （State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University） for software support.
文摘Background The mechanism of acupuncture analgesia in craniotomy has been widely studied. However, the theoretical basis for selection of acupoints has not been examined. In this study, we used the regional homogeneity method blood oxygen level-dependent （BOLD） signals to determine changes in brain activity in response to transcutaneous electrical stimulation on acupoints and non-acupoints in resting state functional magnetic resonance imaging （fMRI）.Methods Twelve healthy volunteers were enrolled in this study. BOLD fMRI scanning of the brain was performed for 306 seconds before and 30 minutes after transcutaneous electrical stimulation on acupoints UB63 （Jinmen）, LV3 （Tai chong）, ST36 （Zusanli）, and GB40 （Qiuxu）. The procedure was repeated after one week with stimulation on non-acupoints （one was 9 above BL67, the second was 12 above BL67 （Kunlun）, the third was 7 above KI3, and the fourth was 10 above KI3 （Taixi））.Results The regional homogeneity in the acupoint group was increased in the left thalamus, caudate, putamen, lentiform nucleus （BA19, 30, 39）, postcentral gyrus, precentral gyrus （BA3, 4, 30, 32）, calcarine fissure, middle temporal gyrus （BA30）, right superior temporal gyrus, inferior temporal gyrus （BA38）, cuneus, and precuneus （BA7, 19） when compared to the non-acupoint group. The regional homogeneity of the acupoint group was decreased in the left cerebellum posterior lobe, middle frontal gyrus （BA10）, double-side precuneus （BA7）, and the postcentral gyrus （BA40）.Conclusions The brain region activated following acupoint stimulation is the ipsilateral pain-related brain region, which may relate to the therapeutic effect of acupuncture on pain relief. Further acupoint stimulation causes different central nervous responses compared to non-acupoint stimulation.
基金Supported by the Project of Beijing University of Chinese Medicine,China(No.JYB22-JS022)
文摘Objective： To investigate the effect of electroacupuncture（EA） treatment on the expression of cyclooxygenase（COX） 2 and microglia in spinal cord by using rat model of neuropathic pain, and to probe into the relationship between COX 2 and microglia. Methods： The rats were randomly divided into 6 groups, including normal control group, model group, sham group, EA 1 group（distant acupoints ＋ local acupoints）, EA 2 group（local acupoints）, and EA 3 group（distant acupoints）. Thermal withdrawal latencies were evaluated at 1 day preoperatively and 3, 5 and 7 days postoperatively. At 7 days postoperatively, the spinal COX 2 m RNA was detected by reverse-transcription polymerase chain reaction. Double immunofluorescent staining technology was applied to screen and verify the relationship between altered COX 2 and microglia. Results： Compared with the model group, thermal withdrawal latencies increased after EA treatment（P〈0.01）. The expressions of COX 2 m RNA were up-regulated in spinal cord of rat on day 7 after surgery（P〈0.05）. Compared with the model group, EA stimulation（EA 1 and EA 2 groups） reversed the up-regulation of COX 2 m RNA expression（P〈0.05）. EA 1 and EA 2 groups might have better treatment effect compared with the EA 3 group. Fluorescent images displayed COX 2 and microglia expressed at common areas. Conclusions： EA was effective in analgesic and anti-inflammatory. EA has decreased the expression of spinal COX 2 m RNA in the trend of the therapeutic effect of ＂distant acupoints ＋ local acupoints＂, and ＂local acupoints＂ intervention may be superior to that of ＂distant acupoints＂ intervention. Microglia may be related to the formation of COX 2.
