FC-DIRECTED ANTIBODY CONJUGATION OF MAGNETIC NANOPARTICLES FOR ENHANCED MOLECULAR TARGETING

In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs)functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody totarget the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aidstheir efficient immunospecific targeting through free Fab portions. The directional specificity ofconjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophagestreated with functionalized MNPs and free anti-HER2 antibody revealed that the antibodymolecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe formolecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.

Global lessons on delivery of primary healthcare services for people with non-communicable diseases:convergent mixed methods

Objective To extract key lessons on primary healthcare(PHC)service delivery strategies for non-communicable diseases(NCD)from the work of researchers funded by the Global Alliance for Chronic Diseases(GACD).Design A convergent mixed methods study that extracted data using a standardised template from research projects funded by the GACD that focused on PHC.The strategies implemented in these studies were mapped onto the PHC Performance Initiative framework.Semistructured qualitative interviews were conducted with researchers from purposefully selected projects to understand the strategies and contextual factors in more depth.Setting PHC contexts from low or middle-income countries(LMIC)as well as vulnerable groups within high-income countries.Projects came from all regions of the world,particularly East Asia and Pacific,sub-Saharan Africa,South Asia,Latin America and Caribbean.Participants The study extracted data on 84 research projects and interviewed researchers from 16 research projects.Results Research projects came from all regions of the world,and mainly focused on diabetes(35.3%),hypertension(28.3%)and mental health(27.6%).Mapped onto the PHC Performance Initiative framework:49.4%focused on high-quality PHC(particularly the comprehensiveness of NCD care,41.2%);41.2%on the availability of PHC services(particularly the competence of healthcare workers,36.5%);35.3%on population health management(particularly community-based services,35.3%);34.1%on facility organisation and management(particularly team-based care,20.0%)and 31.8%on access(particularly digital technology,23.5%).Most common strategies were task shifting and training to improve the comprehensiveness of NCD care through community-based services.Contextual factors related to inputs:infrastructure,equipment and medication,workforce(particularly community health workers),finances,health information systems and digital technology.Conclusion Key strategies and contextual factors to improve PHC service delivery for NCDs in LMICs were identified.These strategies should combine with other strategies to strengthen the PHC system as a whole,while improving care for NCDs.