Light-sheet fluorescence microscopy(LSFM)has played an important role in bio-imaging due to its advantages of high photon efficiency,fast speed,and long-term imaging capabilities.The perpendicular layout between LSFM ...Light-sheet fluorescence microscopy(LSFM)has played an important role in bio-imaging due to its advantages of high photon efficiency,fast speed,and long-term imaging capabilities.The perpendicular layout between LSFM excitation and detection often limits the 3D resolutions as well as their isotropy.Here,we report on a reflective type light-sheet microscope with a mini-prism used as an optical path reflector.The conventional high NA objectives can be used both in excitation and detection with this design.Isotropic resolutions in 3D down to300 nm could be achieved without deconvolution.The proposed method also enables easy transform of a conventional fluorescence microscope to high performance light-sheet microscopy.展开更多
基金National Key Research and Development Program of China(2022YFC3401100,2022YFF0712500)Guangdong Major Project of Basic and Applied Basic Research(2020B0301030009)+5 种基金National Natural Science Foundation of China(12204017,12004012,12004013,12041602,91750203,91850111,92150301)China Postdoctoral Science Foundation(2020M680220,2020M680230)Clinical Medicine Plus X-Young Scholars ProjectPeking UniversityFundamental Research Funds for the Central UniversitiesHigh-performance Computing Platform of Peking University。
文摘Light-sheet fluorescence microscopy(LSFM)has played an important role in bio-imaging due to its advantages of high photon efficiency,fast speed,and long-term imaging capabilities.The perpendicular layout between LSFM excitation and detection often limits the 3D resolutions as well as their isotropy.Here,we report on a reflective type light-sheet microscope with a mini-prism used as an optical path reflector.The conventional high NA objectives can be used both in excitation and detection with this design.Isotropic resolutions in 3D down to300 nm could be achieved without deconvolution.The proposed method also enables easy transform of a conventional fluorescence microscope to high performance light-sheet microscopy.
