It has been shown that in the avian optic tectum, L-glutamate may be an excitatory transmitter, whereas glycine and γ-aminobutyric acid (GABA) may be inhibitory transmitters. Tritiated glycine injections in the avian...It has been shown that in the avian optic tectum, L-glutamate may be an excitatory transmitter, whereas glycine and γ-aminobutyric acid (GABA) may be inhibitory transmitters. Tritiated glycine injections in the avian tectum label the cell bodies within the nucleus isthmus pars parvocellularis (Ipc). Electrical stimulation of Ipc results in the release of exogenous and endogenous glycine into tectal perfusate. It is, therefore, suggested that the isthmotectal pathway uses glycine as its transmitter. Tectal injections of of tritiated GABA also label Ipc cell bodies, indicating that the isthmotectal projection contains GABAergic fibers.展开更多
The miniature endplate currents (MEPC’s) were recorded at the neuromuscular junction of Xenopus laevis embryo neuron-muscle co-cultured cells. These MEPC’s were due to the spontaneous release of acetylcholine from t...The miniature endplate currents (MEPC’s) were recorded at the neuromuscular junction of Xenopus laevis embryo neuron-muscle co-cultured cells. These MEPC’s were due to the spontaneous release of acetylcholine from the nerve terminal. After perfusion with glutamate (10 μmol/L), both frequency and amplitude of the MEPC’s increased. After washing away of glutamate, this effect persisted. We named this phenomena "Long-Term Facilitation".GABA (20 μmol/L) on the other hand had an inhibitory effect on both frequency and amplitude of the MEPC’s. After washing away of GABA, the MEPC frequency and amplitude increased. We named this effect "Post-Potentiation". Local perfusion experiments furthermore indicated that the effect of glutamate was restricted to the neuromuscular junction, the effect of GABA was restricted to the soma.展开更多
基金Project supported by the National Natural Science Foundation of China
文摘It has been shown that in the avian optic tectum, L-glutamate may be an excitatory transmitter, whereas glycine and γ-aminobutyric acid (GABA) may be inhibitory transmitters. Tritiated glycine injections in the avian tectum label the cell bodies within the nucleus isthmus pars parvocellularis (Ipc). Electrical stimulation of Ipc results in the release of exogenous and endogenous glycine into tectal perfusate. It is, therefore, suggested that the isthmotectal pathway uses glycine as its transmitter. Tectal injections of of tritiated GABA also label Ipc cell bodies, indicating that the isthmotectal projection contains GABAergic fibers.
基金Project supported by the National Natural Science Foundation of China.
文摘The miniature endplate currents (MEPC’s) were recorded at the neuromuscular junction of Xenopus laevis embryo neuron-muscle co-cultured cells. These MEPC’s were due to the spontaneous release of acetylcholine from the nerve terminal. After perfusion with glutamate (10 μmol/L), both frequency and amplitude of the MEPC’s increased. After washing away of glutamate, this effect persisted. We named this phenomena "Long-Term Facilitation".GABA (20 μmol/L) on the other hand had an inhibitory effect on both frequency and amplitude of the MEPC’s. After washing away of GABA, the MEPC frequency and amplitude increased. We named this effect "Post-Potentiation". Local perfusion experiments furthermore indicated that the effect of glutamate was restricted to the neuromuscular junction, the effect of GABA was restricted to the soma.
