Objective: Plant-based diets have multiple health benefits for cancers;however, little is known about the association between plant-based dietary patterns and esophageal cancer(EC).This study presents an investigation...Objective: Plant-based diets have multiple health benefits for cancers;however, little is known about the association between plant-based dietary patterns and esophageal cancer(EC).This study presents an investigation of the prospective associations among three predefined indices of plant-based dietary patterns and the risk of EC.Methods: We performed endoscopic screening for 15,709 participants aged 40-69 years from two high-risk areas of China from January 2005 to December 2009 and followed the cohort until December 31, 2022. The overall plant-based diet index(PDI), healthful plant-based diet index(h PDI), and unhealthful plant-based diet index(u PDI), were calculated using survey responses to assess dietary patterns. We applied Cox proportional hazard regression to estimate the multivariable hazard ratios(HRs) and 95% confidence intervals(95% CIs) of EC across 3plant-based diet indices and further stratified the analysis by subgroups.Results: The final study sample included 15,184 participants in the cohort. During a follow-up of 219,365person-years, 176 patients with EC were identified. When the highest quartile was compared with the lowest quartile, the pooled multivariable-adjusted HR of EC was 0.50(95% CI, 0.32-0.77) for h PDI. In addition, the HR per 10-point increase in the h PDI score was 0.42(95% CI, 0.27-0.66) for ECs. Conversely, u PDI was positively associated with the risk of EC, and the HR was 1.80(95% CI, 1.16-2.82). The HR per 10-point increase in the u PDI score was 1.90(95% CI, 1.26-2.88) for ECs. The associations between these scores and the risk of EC were consistent in most subgroups. These results remained robust in sensitivity analyses.Conclusions: A healthy plant-based dietary pattern was associated with a reduced risk of EC. Emphasizing the healthiness and quality of plant-based diets may be important for preventing the development of EC.展开更多
EVI2A has emerged as a significant biomarker in various diseases;however,its biological role and mechanism in kidney renal clear cell carcinoma(KIRC)remains unexplored.We used TCGA and GEO databases to analyze EVI2A g...EVI2A has emerged as a significant biomarker in various diseases;however,its biological role and mechanism in kidney renal clear cell carcinoma(KIRC)remains unexplored.We used TCGA and GEO databases to analyze EVI2A gene expression comprehensively and performed pan-cancer assessments.Clinical relevance was evaluated through Kaplan-Meier analysis and ROC curves.The gene’s immune relevance was explored through analyses of the tumor microenvironment(TME),Tumor Immune Single-cell Hub(TISCH),immune checkpoints,and immunotherapy sensitivity.Our results indicate that EVI2A expression is upregulated in KIRC,showing correlations with tumor grade and T/N/M stage.EVI2A demonstrates high diagnostic accuracy(AUC=0.906)and predicts poor overall and progression-free survival in KIRC patients.Furthermore,EVI2A expression exhibits significant associations with immunity,including TME scores and specific immune cell types such as Tfh cells,CD4 memory T cells,and CD8+T cells.Elevated EVI2A expression suggests increased sensitivity to PD-1/CTLA-4 and tyrosine kinase inhibitors.In vitro assays confirmed the impact of EVI2A on KIRC behavior,with its knockdown resulting in reduced cell proliferation and migration.In conclusion,our comprehensive analysis identifies EVI2A as a promising biomarker and a novel therapeutic target for intervening in KIRC.These findings hold significant implications for further research and potential clinical applications.展开更多
[Objectives]To investigate the mechanisms and pharmacologic effects of Citri Reticulatae Pericarpium against keloids by network pharmacology systematically.[Methods]TCMSP,Uniprot and BATMAN-TCM databases were used to ...[Objectives]To investigate the mechanisms and pharmacologic effects of Citri Reticulatae Pericarpium against keloids by network pharmacology systematically.[Methods]TCMSP,Uniprot and BATMAN-TCM databases were used to obtain the active constituents and targets of Citri Reticulatae Pericarpium."Keloid"was used as key word to search for related therapeutic targets from Drug Bank,OMIM,TTD,and GEO databases.The Chinese medicine compound-target network was constructed by Cytoscape software.Besides,gene ontology(GO)and Kyoto Encyclopedia of genes and genome enrichment analysis were also performed.Afterward,Discovery Studio software was used to assess the interaction of key components and genes.[Results]Five active components of Citri Reticulatae Pericarpium,773 compound targets and 676 keloid treatment targets were obtained in the databases.After the intersection,there are 47 targets of Citri Reticulatae Pericarpium for treating keloids.Hub genes were identified such as MMP9,IL6,TNF,TP53,and VEGFA,which were enriched in tumor necrosis factor-α,nuclear factor kappa-B,and other signaling pathways.The molecular docking stimulation confirmed the interaction between the MMP9 and three components of Citri Reticulatae Pericarpium.[Conclusions]Citri Reticulatae Pericarpium may play an important role in treating keloids through modulating genes and signaling pathways.The present study sheds light on the mechanisms of active compounds of Citri Reticulatae Pericarpium for the treatment of keloids.展开更多
The effects of bagging at different times in Mengyin area on the fruit quality and disease and pest incidence of Huimin short-cut Fuji apple were investigated,with unbagged fruit as the control. The results showed tha...The effects of bagging at different times in Mengyin area on the fruit quality and disease and pest incidence of Huimin short-cut Fuji apple were investigated,with unbagged fruit as the control. The results showed that among the apples bagged on May 5,May 15,May 25,June 2 and June 12,the single fruit weight and fruit shape index of the apples bagged on June 2 were highest,308. 1 g and 0. 868,respectively; the peeled harness of the apples bagged during May 5-25 was higher than that of the control; the peeled harness of the apples bagged on June 2 and June 12 was lower than that of the control; the soluble solids content of bagged fruit was lower than that of unbagged ones,and no significant differences were found among different treatments; the coloring index of the apples bagged on June 2 was up to 100%; the smooth finish index of the apples bagged on May 5 reached 96. 67%,the highest; the red chromatic value( a*) of bagged fruit was higher than that of unbagged ones,and there were no significant differences among different bagging treatments. The earlier the bagging was,the higher the incidence of black spot and bitter pit was. The incidence of black spot and bitter pit in bagged fruit was higher than that in unbagged ones. In conclusion,in Mengyin area of Shandong Province,bagging is better to be carried out on June 2,45 d after the full bloom.展开更多
1.Introduction Hydrogen is an ideal energy carrier to tackle the energy crisis and greenhouse effect,because of its high energy density and low emission.The production,storage and transportation of hydrogen are key fa...1.Introduction Hydrogen is an ideal energy carrier to tackle the energy crisis and greenhouse effect,because of its high energy density and low emission.The production,storage and transportation of hydrogen are key factors to the practical application of hydrogen energy.As the scientific and technological understanding of the electrochemical devices was advancing in the past few decades,water electrolyzers based on the proton exchange membrane (PEM) have attracted much focus for its huge potential on the production of hydrogen via water splitting.PEM electrolyzers use perfluorinated sulfonic acid (PFSA) based membranes as the electrolyte.展开更多
[ Objective] This study aimed to investigate the effects of different water and fertilizer combinations on apple saplings. [ Method] ＇ Tianhong 2＇ Fuji /SH40/Malus robusta Rehd. was used as the experimental material...[ Objective] This study aimed to investigate the effects of different water and fertilizer combinations on apple saplings. [ Method] ＇ Tianhong 2＇ Fuji /SH40/Malus robusta Rehd. was used as the experimental material to analyze the effects of different irrigation modes and combinations of basal fertilizer and dressing fertilizer on tree structure, leaf parameters and photosynthesis of apple saplings. [ Result] The results showed that different water and fertilizer combinations ex- hibited varying effects on tree structure, leaf parameters and photosynthesis of apple saplings. To be specific, applying 432 000 kg/hm2 basal fertilizer, 480 kg/hm2 urea and 915 kg/hmz organic fertilizer ＋ 0 ＋ 915 kg/hm2 organic fertilizer as dressing fertilizer, and 1% urea as leaf fertilizer was conducive to promoting growth of branches and leaves, increasing leaf thickness, individual leaf area and SPAD, and improving photosynthesis of apple saplings under half root irrigation and whole root irrigation conditions. In addition, the effects were more significant under whole root irrigation conditions. [ Conclusion] Selecting the appropriate water and fertilizer combination is conducive to the growth of apple saplings.展开更多
A neural network classification method,and a batch-learning self-organizing map(BLSOM),was established using trinucleotide and tetranucleotide in the hemagglutinin gene sequences of 25 avian influenza viruses isolated...A neural network classification method,and a batch-learning self-organizing map(BLSOM),was established using trinucleotide and tetranucleotide in the hemagglutinin gene sequences of 25 avian influenza viruses isolated in Guangdong Province. Statistical analysis and normalization of the fragment number were done and MATLAB function was used to simulate the human brain thinking for self-organizing learning. When the number of training steps was 100 and above,the strains could be successfully clustered. H_1,H_3,H_5,H_7 and H_9 subtype strains fell within different classes,respectively,and the HA gene cluster map of H_3N_2 and H_7N_9strains was quite similar,suggesting that these strains shared the same origin; H_5N_1 strain was quite different in different years; H_1N_1 and H_9N_2 strains could be clustered into one group,indicating the natural recombinant variation in the two kinds of viruses,thereby providing a reference for high-risk strain screening and traceability.展开更多
Water-saving irrigation is an important way to realize the sustainable development of the apple industry.In order to screen the best irrigation pattern for apple,taking 9-year-old‘Yanfu 10'Fuji/Malus robusta appl...Water-saving irrigation is an important way to realize the sustainable development of the apple industry.In order to screen the best irrigation pattern for apple,taking 9-year-old‘Yanfu 10'Fuji/Malus robusta apple as the material,the effects of different irrigation methods(drip irrigation,sprinkling irrigation,and flood irrigation) on the growth,fruit quality,and yield of apple trees were studied.The results showed that compared with the flood irrigation,drip irrigation and sprinkling irrigation significantly increased the spring shoot length by 14.8%and 9.1%,respectively,and decreased the autumn shoot length by 11.7% and 8.8%,respectively.Drip irrigation and sprinkling irrigation significantly increased the leaf area,chlorophyll content,and leaf weight,the leaf area increased by 3.0% and 1.9%,respectively,the chlorophyll content increased by 13.9% and 11.5%,respectively,and the leaf weight increased by 5.8% and 5.1%,respectively.Drip irrigation and sprinkling irrigation could slightly increase the single fruit weight and fruit shape index,significantly increase the coloring index and smoothness index.The single fruit weight increased by 3.2% and 1.9%,the coloring index increased by 6.1% and 4.1%,the smoothness index increased by 4.7% and 2.8%,and the proportion of red fruit increased by 4.2% and 2.2%,respectively.The content of soluble solids in drip irrigation and sprinkling irrigation was significantly higher than that in flood irrigation,which was 13.0% and 2.6% higher than CK,respectively.The fruit hardness in drip irrigation and sprinkling irrigation was 7.9% and 2.2% higher than CK,respectively,and that in drip irrigation increased significantly.The yield in drip irrigation and sprinkling irrigation was 12.1% and 8.2% higher than CK,respectively.In conclusion,drip irrigation and sprinkling irrigation could promote the growth of apple trees,improve the fruit quality,and increase the yield of apple trees,and the effect of drip irrigation is better than sprinkling irrigation.展开更多
In order to optimize and transform closed mature apple orchards with standard rootstocks and improve the quality of fruit,taking a closed Red Fuji apple orchard as the test object,the effects of different density-redu...In order to optimize and transform closed mature apple orchards with standard rootstocks and improve the quality of fruit,taking a closed Red Fuji apple orchard as the test object,the effects of different density-reducing methods(deinterlacing,removing every other plant in each row,removing every other plant every other row)on the canopy microenvironment,tree structure,leaf photosynthesis and fruit quality were studied.The results showed that different density-reducing methods significantly reduced the orchard coverage and increased the crown transmittance.Among them,the deinterlacing treatment was the best in improving the population structure of the closed orchard,as it reduced the orchard coverage rate by 55.68 percentage points and the canopy transmittance by 82.38 percentage points,compared with the control(CK).Different density-reducing methods all could significantly reduce the branch amount in the closed orchard and optimized the branch composition.The three density-reducing methods decreased the number of branches per plant by 18.96%,12.41%and 19.58%,respectively,compared with the CK.And compared with the CK,the proportion of short branches and leafy branches to the total branches was increased by 17.13%,14.27%and 7.37%,respectively,and the proportion of long branches and developmental branches to the total branches was decreased by 24.47%,18.04%and 10.79%,respectively.The effects of the different density-reducing methods on the temperature,relative light intensity,SPAD and leaf photosynthetic rate in canopies all followed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing<removing every other plant in each row<removing every other plant every other row<CK.The average single fruit weight(238.3 g),coloring index(89.2),smoothness index(83.2),soluble solid content(15.1%)and high quality fruit rate(82.4%)of the deinterlacing treatment were higher than those of other treatments,and the values were 18.2%,11.4%,5.85%,26.9%and 25.2%higher than the CK,respectively.The use of dein￣terlacing to reduce density is the best for improving the microenvironment of closed apple orchards and improving the photosynthetic efficiency and fruit quality.展开更多
Objective:To investigate the anatomical basis of Rubens'flap based on the deep circumflex iliac artery,and to apply more donor site tissue amount for big chest wall defect.Methods:Gross anatomical study was carrie...Objective:To investigate the anatomical basis of Rubens'flap based on the deep circumflex iliac artery,and to apply more donor site tissue amount for big chest wall defect.Methods:Gross anatomical study was carried on 8 sides of fresh specimens of 4 cases and data was measured by mean of the Vernier caliper.Besides,the surgical simulation was carried on 1 specimen(2 sides).Results:At the inguinal segment,the via artery gave off(9.16±6.22)branches;the diameter of the origin was(3.97±0.86)mm;the distance from starting point to the first branch was(15.87±9.24)mm;amount of osteomusculocutaneous branch was 3.12±1.34;the biggest diameter of perforator was(1.48±1.02)mm;pedicle length was(132.51±48.24)mm.In the surgical simulation,the layers of Ruben's flap from up to down ranged in skin,subcutaneous tissue,obliquus externus abdominis,oblique internal abdominis and transversus abdominis.Conclusion:Rubens'flap,with large tissue amount,based on the deep circumflex iliac artery,near to traditional abdominal flap,has a good clinical application prospect in breast reconstruction and repair of big chest wall defect,for its thin waist effect and slight donor site defect.展开更多
土壤含水量是气候、水文和生态等研究的重要参数。地表温度-植被指数特征空间法是遥感监测土壤含水量的常用方法。以欧洲伊比利亚半岛为研究区,使用MSG-SEVIRI(Meteosat Second Generation-Spinning Enhanced Visible and Infrared Imag...土壤含水量是气候、水文和生态等研究的重要参数。地表温度-植被指数特征空间法是遥感监测土壤含水量的常用方法。以欧洲伊比利亚半岛为研究区,使用MSG-SEVIRI(Meteosat Second Generation-Spinning Enhanced Visible and Infrared Imager)晴空数据,构建地表温度日较差-植被覆盖度特征空间。在此特征空间上,结合研究区土壤质地数据,建立土壤含水量反演模型反演土壤体积含水量。利用西班牙REMEDHUS (REd de MEDiciòn de la HUmedad del Suelo)土壤含水量观测网络的实测数据对反演结果进行验证,均方根误差均在0. 05 m^3/m^3以内,具有较高的精度。与常用的地表温度-植被覆盖度特征空间的结果对比证明,以地表温度日较差替代地表温度,能够减小地表温度反演误差导致的土壤含水量估算误差,从而提高土壤含水量反演精度。展开更多
基金supported by grants from the Beijing Nova Program (No. Z201100006820069)CAMS Innovation Fund for Medical Sciences (CIFMS, No. 2021-I2M-1-023, 2021-I2M-1-010)Talent Incentive Program of Cancer Hospital Chinese Academy of Medical Sciences (Hope Star)。
文摘Objective: Plant-based diets have multiple health benefits for cancers;however, little is known about the association between plant-based dietary patterns and esophageal cancer(EC).This study presents an investigation of the prospective associations among three predefined indices of plant-based dietary patterns and the risk of EC.Methods: We performed endoscopic screening for 15,709 participants aged 40-69 years from two high-risk areas of China from January 2005 to December 2009 and followed the cohort until December 31, 2022. The overall plant-based diet index(PDI), healthful plant-based diet index(h PDI), and unhealthful plant-based diet index(u PDI), were calculated using survey responses to assess dietary patterns. We applied Cox proportional hazard regression to estimate the multivariable hazard ratios(HRs) and 95% confidence intervals(95% CIs) of EC across 3plant-based diet indices and further stratified the analysis by subgroups.Results: The final study sample included 15,184 participants in the cohort. During a follow-up of 219,365person-years, 176 patients with EC were identified. When the highest quartile was compared with the lowest quartile, the pooled multivariable-adjusted HR of EC was 0.50(95% CI, 0.32-0.77) for h PDI. In addition, the HR per 10-point increase in the h PDI score was 0.42(95% CI, 0.27-0.66) for ECs. Conversely, u PDI was positively associated with the risk of EC, and the HR was 1.80(95% CI, 1.16-2.82). The HR per 10-point increase in the u PDI score was 1.90(95% CI, 1.26-2.88) for ECs. The associations between these scores and the risk of EC were consistent in most subgroups. These results remained robust in sensitivity analyses.Conclusions: A healthy plant-based dietary pattern was associated with a reduced risk of EC. Emphasizing the healthiness and quality of plant-based diets may be important for preventing the development of EC.
基金the Ethics Committee of the First Affiliated Hospital of Nanchang University(Approval:(2023)CDYFYYLK(03-013)).
文摘EVI2A has emerged as a significant biomarker in various diseases;however,its biological role and mechanism in kidney renal clear cell carcinoma(KIRC)remains unexplored.We used TCGA and GEO databases to analyze EVI2A gene expression comprehensively and performed pan-cancer assessments.Clinical relevance was evaluated through Kaplan-Meier analysis and ROC curves.The gene’s immune relevance was explored through analyses of the tumor microenvironment(TME),Tumor Immune Single-cell Hub(TISCH),immune checkpoints,and immunotherapy sensitivity.Our results indicate that EVI2A expression is upregulated in KIRC,showing correlations with tumor grade and T/N/M stage.EVI2A demonstrates high diagnostic accuracy(AUC=0.906)and predicts poor overall and progression-free survival in KIRC patients.Furthermore,EVI2A expression exhibits significant associations with immunity,including TME scores and specific immune cell types such as Tfh cells,CD4 memory T cells,and CD8+T cells.Elevated EVI2A expression suggests increased sensitivity to PD-1/CTLA-4 and tyrosine kinase inhibitors.In vitro assays confirmed the impact of EVI2A on KIRC behavior,with its knockdown resulting in reduced cell proliferation and migration.In conclusion,our comprehensive analysis identifies EVI2A as a promising biomarker and a novel therapeutic target for intervening in KIRC.These findings hold significant implications for further research and potential clinical applications.
基金Supported by Central Government Funds of Guiding Local Scientific and Technological Development for Sichuan Province(2021ZYD0057).
文摘[Objectives]To investigate the mechanisms and pharmacologic effects of Citri Reticulatae Pericarpium against keloids by network pharmacology systematically.[Methods]TCMSP,Uniprot and BATMAN-TCM databases were used to obtain the active constituents and targets of Citri Reticulatae Pericarpium."Keloid"was used as key word to search for related therapeutic targets from Drug Bank,OMIM,TTD,and GEO databases.The Chinese medicine compound-target network was constructed by Cytoscape software.Besides,gene ontology(GO)and Kyoto Encyclopedia of genes and genome enrichment analysis were also performed.Afterward,Discovery Studio software was used to assess the interaction of key components and genes.[Results]Five active components of Citri Reticulatae Pericarpium,773 compound targets and 676 keloid treatment targets were obtained in the databases.After the intersection,there are 47 targets of Citri Reticulatae Pericarpium for treating keloids.Hub genes were identified such as MMP9,IL6,TNF,TP53,and VEGFA,which were enriched in tumor necrosis factor-α,nuclear factor kappa-B,and other signaling pathways.The molecular docking stimulation confirmed the interaction between the MMP9 and three components of Citri Reticulatae Pericarpium.[Conclusions]Citri Reticulatae Pericarpium may play an important role in treating keloids through modulating genes and signaling pathways.The present study sheds light on the mechanisms of active compounds of Citri Reticulatae Pericarpium for the treatment of keloids.
基金Supported by Earmarked Fund for China Agriculture Research System(CARS-27)Key Research and Development Project of Shandong Province(2017CXGC0210)Youth Research Fund of Shandong Institute of Pomology(2018KY07)
文摘The effects of bagging at different times in Mengyin area on the fruit quality and disease and pest incidence of Huimin short-cut Fuji apple were investigated,with unbagged fruit as the control. The results showed that among the apples bagged on May 5,May 15,May 25,June 2 and June 12,the single fruit weight and fruit shape index of the apples bagged on June 2 were highest,308. 1 g and 0. 868,respectively; the peeled harness of the apples bagged during May 5-25 was higher than that of the control; the peeled harness of the apples bagged on June 2 and June 12 was lower than that of the control; the soluble solids content of bagged fruit was lower than that of unbagged ones,and no significant differences were found among different treatments; the coloring index of the apples bagged on June 2 was up to 100%; the smooth finish index of the apples bagged on May 5 reached 96. 67%,the highest; the red chromatic value( a*) of bagged fruit was higher than that of unbagged ones,and there were no significant differences among different bagging treatments. The earlier the bagging was,the higher the incidence of black spot and bitter pit was. The incidence of black spot and bitter pit in bagged fruit was higher than that in unbagged ones. In conclusion,in Mengyin area of Shandong Province,bagging is better to be carried out on June 2,45 d after the full bloom.
基金supported by the National Key R&D Program of China(2021YFA1500900,2020YFA0710000)the National Natural Science Foundation of China(22172047,22002039,21825201 and U19A2017)+3 种基金the Provincial Natural Science Foundation of Hunan(2021JJ30089,2016TP1009 and 2020JJ5045)the China Postdoctoral Science Foundation(2019M662759,2020M682541 and 2020M682549)the Shenzhen Science and Technology Program(JCYJ20210324122209025)the Changsha Municipal Natural Science Foundation(kq2107008 and kq2007009)。
文摘1.Introduction Hydrogen is an ideal energy carrier to tackle the energy crisis and greenhouse effect,because of its high energy density and low emission.The production,storage and transportation of hydrogen are key factors to the practical application of hydrogen energy.As the scientific and technological understanding of the electrochemical devices was advancing in the past few decades,water electrolyzers based on the proton exchange membrane (PEM) have attracted much focus for its huge potential on the production of hydrogen via water splitting.PEM electrolyzers use perfluorinated sulfonic acid (PFSA) based membranes as the electrolyte.
基金Supported by National Modern Agricultural(Apple)Industry Technology System of China(CARS-28)
文摘[ Objective] This study aimed to investigate the effects of different water and fertilizer combinations on apple saplings. [ Method] ＇ Tianhong 2＇ Fuji /SH40/Malus robusta Rehd. was used as the experimental material to analyze the effects of different irrigation modes and combinations of basal fertilizer and dressing fertilizer on tree structure, leaf parameters and photosynthesis of apple saplings. [ Result] The results showed that different water and fertilizer combinations ex- hibited varying effects on tree structure, leaf parameters and photosynthesis of apple saplings. To be specific, applying 432 000 kg/hm2 basal fertilizer, 480 kg/hm2 urea and 915 kg/hmz organic fertilizer ＋ 0 ＋ 915 kg/hm2 organic fertilizer as dressing fertilizer, and 1% urea as leaf fertilizer was conducive to promoting growth of branches and leaves, increasing leaf thickness, individual leaf area and SPAD, and improving photosynthesis of apple saplings under half root irrigation and whole root irrigation conditions. In addition, the effects were more significant under whole root irrigation conditions. [ Conclusion] Selecting the appropriate water and fertilizer combination is conducive to the growth of apple saplings.
基金Supported by National Entry-Exit Inspection and Quarantine Research Project(2015IK054)Special Project of Ministry of Science and Technology for Development of Major Scientific Instruments and Equipments(2012YQ0901-9705)
文摘A neural network classification method,and a batch-learning self-organizing map(BLSOM),was established using trinucleotide and tetranucleotide in the hemagglutinin gene sequences of 25 avian influenza viruses isolated in Guangdong Province. Statistical analysis and normalization of the fragment number were done and MATLAB function was used to simulate the human brain thinking for self-organizing learning. When the number of training steps was 100 and above,the strains could be successfully clustered. H_1,H_3,H_5,H_7 and H_9 subtype strains fell within different classes,respectively,and the HA gene cluster map of H_3N_2 and H_7N_9strains was quite similar,suggesting that these strains shared the same origin; H_5N_1 strain was quite different in different years; H_1N_1 and H_9N_2 strains could be clustered into one group,indicating the natural recombinant variation in the two kinds of viruses,thereby providing a reference for high-risk strain screening and traceability.
基金Supported by National Key Technologies R&D Program of China(2014BAD16B02-2)Key Research and Development Program of Shandong Province(2017CXGC0210)+1 种基金Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2017D01)Modern Agricultural Industry Technology System of China for Apple(CARS-27)
文摘Water-saving irrigation is an important way to realize the sustainable development of the apple industry.In order to screen the best irrigation pattern for apple,taking 9-year-old‘Yanfu 10'Fuji/Malus robusta apple as the material,the effects of different irrigation methods(drip irrigation,sprinkling irrigation,and flood irrigation) on the growth,fruit quality,and yield of apple trees were studied.The results showed that compared with the flood irrigation,drip irrigation and sprinkling irrigation significantly increased the spring shoot length by 14.8%and 9.1%,respectively,and decreased the autumn shoot length by 11.7% and 8.8%,respectively.Drip irrigation and sprinkling irrigation significantly increased the leaf area,chlorophyll content,and leaf weight,the leaf area increased by 3.0% and 1.9%,respectively,the chlorophyll content increased by 13.9% and 11.5%,respectively,and the leaf weight increased by 5.8% and 5.1%,respectively.Drip irrigation and sprinkling irrigation could slightly increase the single fruit weight and fruit shape index,significantly increase the coloring index and smoothness index.The single fruit weight increased by 3.2% and 1.9%,the coloring index increased by 6.1% and 4.1%,the smoothness index increased by 4.7% and 2.8%,and the proportion of red fruit increased by 4.2% and 2.2%,respectively.The content of soluble solids in drip irrigation and sprinkling irrigation was significantly higher than that in flood irrigation,which was 13.0% and 2.6% higher than CK,respectively.The fruit hardness in drip irrigation and sprinkling irrigation was 7.9% and 2.2% higher than CK,respectively,and that in drip irrigation increased significantly.The yield in drip irrigation and sprinkling irrigation was 12.1% and 8.2% higher than CK,respectively.In conclusion,drip irrigation and sprinkling irrigation could promote the growth of apple trees,improve the fruit quality,and increase the yield of apple trees,and the effect of drip irrigation is better than sprinkling irrigation.
基金Supported by Key Research and Development Program of Shandong Province(2017CXGC0210)Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2017D01)+3 种基金National Key R&D Program of China(2016YFD0201100)National Natural Science Foundation of China(31600021)Modern Agricultural Industry Technology System of China for Apple(CARS-27)Dongying Science and Technology Program(2015GG0104)
文摘In order to optimize and transform closed mature apple orchards with standard rootstocks and improve the quality of fruit,taking a closed Red Fuji apple orchard as the test object,the effects of different density-reducing methods(deinterlacing,removing every other plant in each row,removing every other plant every other row)on the canopy microenvironment,tree structure,leaf photosynthesis and fruit quality were studied.The results showed that different density-reducing methods significantly reduced the orchard coverage and increased the crown transmittance.Among them,the deinterlacing treatment was the best in improving the population structure of the closed orchard,as it reduced the orchard coverage rate by 55.68 percentage points and the canopy transmittance by 82.38 percentage points,compared with the control(CK).Different density-reducing methods all could significantly reduce the branch amount in the closed orchard and optimized the branch composition.The three density-reducing methods decreased the number of branches per plant by 18.96%,12.41%and 19.58%,respectively,compared with the CK.And compared with the CK,the proportion of short branches and leafy branches to the total branches was increased by 17.13%,14.27%and 7.37%,respectively,and the proportion of long branches and developmental branches to the total branches was decreased by 24.47%,18.04%and 10.79%,respectively.The effects of the different density-reducing methods on the temperature,relative light intensity,SPAD and leaf photosynthetic rate in canopies all followed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing<removing every other plant in each row<removing every other plant every other row<CK.The average single fruit weight(238.3 g),coloring index(89.2),smoothness index(83.2),soluble solid content(15.1%)and high quality fruit rate(82.4%)of the deinterlacing treatment were higher than those of other treatments,and the values were 18.2%,11.4%,5.85%,26.9%and 25.2%higher than the CK,respectively.The use of dein￣terlacing to reduce density is the best for improving the microenvironment of closed apple orchards and improving the photosynthetic efficiency and fruit quality.
基金This study was supported by Capital Development Fund(Grant No.20093010)Clinical Characteristic Application of Capital City(Grant No.Z111107058811097)+2 种基金Research and Development Fund of Peking University People's Hospital(Grant No.RDC2014-27)Research and Development Fund of Peking University People's Hospital(Discipline Cultivation Project)(Grant No.RDD2016-05)Youth Fund Project of Natural Science Foundation of Hainan Province(2018)(Grant No.818QN313).
文摘Objective:To investigate the anatomical basis of Rubens'flap based on the deep circumflex iliac artery,and to apply more donor site tissue amount for big chest wall defect.Methods:Gross anatomical study was carried on 8 sides of fresh specimens of 4 cases and data was measured by mean of the Vernier caliper.Besides,the surgical simulation was carried on 1 specimen(2 sides).Results:At the inguinal segment,the via artery gave off(9.16±6.22)branches;the diameter of the origin was(3.97±0.86)mm;the distance from starting point to the first branch was(15.87±9.24)mm;amount of osteomusculocutaneous branch was 3.12±1.34;the biggest diameter of perforator was(1.48±1.02)mm;pedicle length was(132.51±48.24)mm.In the surgical simulation,the layers of Ruben's flap from up to down ranged in skin,subcutaneous tissue,obliquus externus abdominis,oblique internal abdominis and transversus abdominis.Conclusion:Rubens'flap,with large tissue amount,based on the deep circumflex iliac artery,near to traditional abdominal flap,has a good clinical application prospect in breast reconstruction and repair of big chest wall defect,for its thin waist effect and slight donor site defect.
文摘土壤含水量是气候、水文和生态等研究的重要参数。地表温度-植被指数特征空间法是遥感监测土壤含水量的常用方法。以欧洲伊比利亚半岛为研究区,使用MSG-SEVIRI(Meteosat Second Generation-Spinning Enhanced Visible and Infrared Imager)晴空数据,构建地表温度日较差-植被覆盖度特征空间。在此特征空间上,结合研究区土壤质地数据,建立土壤含水量反演模型反演土壤体积含水量。利用西班牙REMEDHUS (REd de MEDiciòn de la HUmedad del Suelo)土壤含水量观测网络的实测数据对反演结果进行验证,均方根误差均在0. 05 m^3/m^3以内,具有较高的精度。与常用的地表温度-植被覆盖度特征空间的结果对比证明,以地表温度日较差替代地表温度,能够减小地表温度反演误差导致的土壤含水量估算误差,从而提高土壤含水量反演精度。
