衰弱对社区获得性肺炎患者的临床疗效及生活质量的影响研究

目的:探究衰弱对社区获得性肺炎患者的临床疗效及生活质量的影响。方法:选取2022年1月至2023年8月期间我院收治的60例获得性肺炎患者作为研究对象。按照患者的衰弱表型评估方法(Fried衰弱评估)评分是否小于3分将患者分为非衰弱组(n=23)和衰弱组(n=37)。根据《中国成人社区获得性肺炎诊断和治疗指南》的建议,对患者采取相应的治疗措施。比较两组患者临床疗效、临床症状消退时间、肺功能[第1秒用力呼气量(First second forced expiratory volume,FEV1)、用力肺活量(Forced vital capacity,FVC)及FEV1与FVC的比值(Ratio of FEV1 to FVC,FEV1/FVC)]、生活质量[生活质量量表(SF-36)]的差异。结果:治疗后4 w,衰弱组的总有效率明显低于非衰弱组(P<0.05);衰弱组患者的临床症状消失时间明显长于非衰弱组(P<0.05);两组患者FEV1、FVC、FEV1/FVC肺功能明显高于治疗前,但衰弱组明显低于非衰弱组(P<0.05);衰弱组患者的生活质量指标评分明显低于非衰弱组(P<0.05)。结论:针对社区获得性肺炎患者,衰弱对临床疗效有负面影响,会延长患者的临床症状消退时间,影响患者肺功能恢复,降低患者的生活质量。

双层CNN三维刚性与非刚性模型相似性分析

三维模型相似性分析是计算机视觉中的重点问题,如何构建其形状特征和对比函数是难点。随着深度学习出现,通过神经网络自动提取模型特征成为研究热点。构建了双层CNN网络,首先利用热核特征函数分别构建带有颜色的刚性和非刚性训练集与测试集,其次将数据集通过双层CNN网络进行模型训练,第一层实现类别初步判定,第二层实现同一模型刚性与非刚性形变区分。为了提高分类准确度,对初步分类错误的模型引入阈值判定,将其直接排除。通过实验分析,双层CNN网络刚性与非刚性的判别准确率达到99%。实验证明,该方法在模型相似性分析上是鲁棒的,且提取的特征不受人工干扰。

Science Letters:IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1

Insulin-like growth factor binding-protein-7 （IGFBP7） was obtained from our previous colonic adenocarcinoma （CRC） and normal mucosa suppression subtraction hybridization （SSH） cDNA libraries. By RT-PCR and immunohistochemistry, we found that IGFBP7 was overexpressed in CRC tissue compared to normal tissue. However, our in vitro experiments performed in 10 CRC cell lines showed that IGFBP7 expressed only in SW480 and Caco2 cell lines, which implied an underlying reversible regulatory mechanism. Using methylation-specific PCR （MSP） and bisulfite sodium PCR （BSP）, we found that its expression was associated with DNA hypomethylation of exonl. This was further supported by the in vitro study which showed restored IGFBP7 expression after demethylation agent 5-aza-2＇-deoxycytidine treatment. Correlation analysis between IGFBP7 expression and prognosis indicated that overexpression of IGFBP7 in CRC tissue correlated with favourable survival. Investigation of the functional role of IGFBP7 through transfection studies showed that IGFBP7 protein could inhibit growth rate, decrease colony formation activity, and induce apoptosis in RKO and SW620 cells, suggesting it a potential tumor suppressor protein in colorectal carcinogenesis. In conclusion, our study clearly demonstrated that IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1.

Review:Anticancer effects of Chinese herbal medicine,science or myth?

Currently there is considerable interest among oncologists to find anticancer drugs in Chinese herbal medicine (CHM). In the past, clinical data showed that some herbs possessed anticancer properties, but western scientists have doubted the scientific validity of CHM due to the lack of scientific evidence from their perspective. Recently there have been encouraging results, from a western perspective, in the cancer research field regarding the anticancer effects of CHM. Experiments showed that CHM played its anticancer role by inducing apoptosis and differentiation, enhancing the immune system, inhibiting angiogenesis, reversing multidrug resistance (MDR), etc. Clinical trials demonstrated that CHM could improve survival, increase tumor response, improve quality of life, or reduce chemotherapy toxicity, although much remained to be determined regarding the objective effects of CHM in human in the context of clinical trials. Interestingly, both laboratory experiments and clinical trials have demonstrated that when combined with chemotherapy, CHM could raise the efficacy level and lower toxic reactions. These facts raised the feasibility of the combination of herbal medicines and chemotherapy, although much remained to be investigated in this area.

神农香菊茎叶废弃物中多糖与总黄酮的含量测定和抗氧化活性研究

本研究对神农香菊废弃物茎叶部位中多糖和总黄酮进行了含量测定和抗氧化活性研究。通过提取神农香菊茎叶部位中总多糖与黄酮,并根据苯酚-硫酸法和硝酸铝-亚硝酸钠比色法测定含量,采用DPPH自由基清除能力测定、羟自由基清除能力测定、铁的总还原能力测定方法(FRAP法)进行抗氧化活性测定。结果表明,神农香菊废弃部位茎叶中多糖和总黄酮的含量分别为2.86%和4.02%,且均显示出较好的抗氧化活性,其中总黄酮对DPPH自由基的清除能力和羟自由基的清除能力的IC50分别为0.029±0.28 mg/mL和0.042±0.13 mg/mL,多糖对DPPH自由基的清除能力和羟自由基的清除能力的IC50分别为0.051±0.13 mg/mL和0.054±0.22 mg/mL,FRAP法分析表明在同样的浓度下(0.20 mg/mL)黄酮的总抗氧化能力(FRAP值1.06 mmol/L)较多糖(FRAP值0.85 mmol/L)的强。本实验研究的结果为神农香菊茎叶废弃物的利用及开发天然抗氧化剂提供了科学依据。

恩施道地药材厚朴有效成分含量差异研究

以生长周期5年为界限,研究恩施道地药材厚朴中有效成分含量的差异,为厚朴的人工培育类型选择提供参考依据。采取Agilent Intermax C-18(250mm*4.6mm,5μm)流动相(甲醇∶水=78∶22),检测波长为294nm,体积流量1 mL/min,柱温30℃,进样体积10μL,进行HPLC检测,测定尖叶厚朴、中间型厚朴和凹叶厚朴中厚朴酚及和厚朴酚的含量。结果显示测定的绝大多数植株均含有较高的厚朴酚及和厚朴酚,其中,尖叶厚朴中和厚朴酚及厚朴酚总含量较高,最高可达5.21%,中间型厚朴中和厚朴酚及厚朴酚总含量其次,最高含量为3.47%,凹叶厚朴中和厚朴酚及厚朴酚总含量较低,总含量值为3.14%。因此,建议优先考虑尖叶厚朴作为移植培育的品种,减少凹叶厚朴的种植。

中国企业出口优势升级路径及其影响机制——以通讯设备制造业为例

文章基于异质企业贸易理论构建产品质量和价格双重维度的企业出口优势指标,利用通讯设备制造业全球贸易数据,运用多元Ordered Logit计量模型,考察企业生产率和出口目的国质量偏好对中国企业出口优势选择的影响机制。结果显示:随着生产效率的提升,企业选择价格优势的可能性提高,从价格优势向质量优势转变的可能性降低。随着目的国市场质量偏好的提升,企业选择价格优势的可能性降低,从价格优势向质量优势转变的可能性升高。研究结果表明当市场需求偏好无差异时,高生产率的中国企业更倾向于采用传统的价格竞争策略。当市场需求偏好存在差异时,目的国高质量偏好会倒逼企业从价格优势向质量优势转变。

HPLC法测定达克替尼含量及有关物质

目的:建立测定达克替尼含量及有关物质的高效液相色谱法(high performance liquid chromatography,HPLC),以控制达克替尼的质量标准。方法:采用Agilent Eclipse XDB×C18柱(250 mm×4.6 mm,5μm);流动相为乙酸铵水溶液(30 mmol·L-1)-甲醇(20∶80);流速:1.0 mL·min-1;柱温为25℃;检测波长为252 nm;进样体积为20μL。结果:本方法能较好地分离达克替尼和杂质峰,达克替尼浓度在48.40~242.0μg·mL-1浓度范围内,线性关系良好(r=0.999 9);精密度、稳定性、重复性的RSD均<1%;回收率为99.79%,RSD=0.39%(n=9)。结论:该操作方法简便、快捷、重复性好,可用于达克替尼的含量测定。