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<i>In Vitro</i>Plant Regeneration of <i>Morus indica</i>L. cv. V1 Using Leaf Explant 被引量:1
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作者 Melur Kodandaram Raghunath Karaba N. Nataraja +3 位作者 Jyothi Sainath Meghana radha sivarajan sajeevan Mala V. Rajan Syed Mashayak Hussaine Qadri 《American Journal of Plant Sciences》 2013年第10期2001-2005,共5页
Adventitious bud induction and plantlet regeneration were studied in a popular mulberry variety, V1 using leaf as an explant. Fully expanded leaf explants were cultured on Murashige and Skoog’s (MS) medium supplement... Adventitious bud induction and plantlet regeneration were studied in a popular mulberry variety, V1 using leaf as an explant. Fully expanded leaf explants were cultured on Murashige and Skoog’s (MS) medium supplemented with thidiazuron (TDZ) (0.5-4.0 mg/l), 6-benzylaminopurine (BAP) (0.5-2.0 mg/l), indole acetic acid (IAA) (2.0 mg/l), gibberlic acid (GA3) (1.0-2.0 mg/l) silver nitrate (AgNO3) (2.0 mg/l) and different carbon sources such as sucrose, fructose and glucose (10%-30%) either individually or in combination to induce adventitious buds and regeneration. The highest percentage (63%) of adventitious bud formation and regeneration (68%) was achieved in the medium containing MS with TDZ (1.0 mg/l), IAA (2.0 mg/l) and AgNO3 (2.0 mg/l). For subsequent regeneration and shoot elongation the MS medium having BAP (1.0 mg/l), GA3 (2.0 mg/l) and AgNO3 (2.0 mg/l) was found to be suitable. Amongst the carbon sources tested, the most suitable carbon source was found to be sucrose (3%) followed by fructose (2%) for adventitious bud formation. Excised in vitro shoots were rooted (60%-80%) in half strength MS medium supplemented with indole-3-butyric acid (1.0 mg/l). The well rooted plantlets were hardened in soil + sand + farm yard manure (FYM) mixture with a success rate of 70%-90%. Since in vitro regeneration is highly genotype-dependent in mulberry, the standardized protocol can be effectively used for further improvement of this leading genotype using biotechnological approaches. 展开更多
关键词 In Vitro Regeneration Adventitious Bud THIDIAZURON Mulberry cv. V1 Carbon Source
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An Efficient Protocol for Total RNA Isolation from Healthy and Stressed Tissues of Mulberry (<i>Morus</i>sp.) and Other Species 被引量:1
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作者 radha sivarajan sajeevan Manchanahally Byrappa Shivanna Karaba N. Nataraja 《American Journal of Plant Sciences》 2014年第13期2057-2065,共9页
Extraction of quality RNA for molecular biology applications from perennial woody plants like mulberry is complicated due to the presence of high polysaccharides, polyphenols and other secondary metabolites. Since the... Extraction of quality RNA for molecular biology applications from perennial woody plants like mulberry is complicated due to the presence of high polysaccharides, polyphenols and other secondary metabolites. Since the existing methods failed to yield quality RNA in sufficient quantity from leaf and root tissues of mulberry, in this study, we modified the CTAB-based protocol. The standardised protocol yielded high quantity (520.00 μg/g fresh weight of leaf tissue) of quality RNA and the RNA extracted was suitable for all downstream applications such as cDNA synthesis, PCR and whole transcriptome analysis. The method developed was also found to be useful for isolating good quality and quantity total RNA from desiccated and salinity stressed leaf tissues of mulberry. The protocol was also applied successfully to isolate total RNA from leaf tissues of other species such as cardamom, papaya and rice. 展开更多
关键词 MULBERRY CARDAMOM PAPAYA Rice Dehydration Salinity RNA Isolation
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Identification and Characterization of OsWRKY72 Variant inIndica Genotypes
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作者 narasimha ashwini radha sivarajan sajeevan +1 位作者 makarla udayakumar karaba nalkur nataraja 《Rice science》 SCIE CSCD 2016年第6期297-305,共9页
Plant WRKY transcription factors (TFs) constitute one of the largest families of proteinsinvolved in biotic and abiotic stress responses. These TFs have a conserved 60 amino acid WRKYdomain at the N-terminal and a z... Plant WRKY transcription factors (TFs) constitute one of the largest families of proteinsinvolved in biotic and abiotic stress responses. These TFs have a conserved 60 amino acid WRKYdomain at the N-terminal and a zinc finger motif at the C-terminal. To examine the relevance ofOsWRKY72 in imparting salinity stress tolerance, two indica rice genotypes, Rasi (tolerant genotype)and Tellahamsa (susceptible genotype), were used. In Rasi seedlings at 12 h under 100 mmol/L NaClstress, OsWRKY72 expression was up-regulated, whereas in Tellahamsa, it was highly up-regulated atlethal stress. Full-length OsWRKY72 cDNA was cloned from these two rice genotypes for furtheranalysis. We identified a variant, termed as OsWRKY72b that carries an additional sequence of 111 bpwithin the WRKY domain. Expression of OsWRKY72b was higher under salinity stress in Rasi than inTellahamsa. Disorder prediction of OsWRKY72b showed that the additional sequence in the WRKYdomain is ordered thereby maintaining the tertiary structure that might interact with the major groove ofDNA. Prediction of phosphorylation sites in OsWRKY72b indicated that a few serine residues could bethe potential phosphorylation sites. In this study, we firstly reported a OsWRKY72 variant that couldhave a role in abiotic stress responses. 展开更多
关键词 Oryza sativa indica genotype OsWRKY72 gene SALINITY transcription factor
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