Much has been learned about how plants acclimate to stressful environments,but the molecular basis of stress adaptation and the potential involvement of epigenetic regulation remain poorly understood.Here,we examined ...Much has been learned about how plants acclimate to stressful environments,but the molecular basis of stress adaptation and the potential involvement of epigenetic regulation remain poorly understood.Here,we examined if salt stress induces mutagenesis in suspension cultured plant cells and if DNA methylation affects the mutagenesis using whole genome resequencing analysis.We generated suspension cell cultures from two Arabidopsis DNA methylation-deficient mutants and wild-type plants,and subjected the cultured cells to stepwise increases in salt stress intensity over 40 culture cycles.We show that ddc(drm1 drm2 cmt3)mutant cells can adapt to grow in 175 mM NaCl-containing growth medium and exhibit higher adaptability compared to wild type Col-0 and nrpe1 cells,which can adapt to grow in only 125mM NaCl-containing growth medium.Salt treated nrpe1 and ddc cells but not wild type cells accumulate more mutations compared with their respective untreated cells.There is no enrichment of stress responsive genes in the list of mutated genes in salt treated cells compared to the list of mutated genes in untreated cells.Our results suggest that DNA methylation prevents the induction of mutagenesis by salt stress in plant cells during stress adaptation.展开更多
Following publication of this article(Zhu et al.2021),it is noticed that the article contained an error.The affiliation of the author Ray A.Bressan should be below:4.Department of Horticulture and Landscape Architectu...Following publication of this article(Zhu et al.2021),it is noticed that the article contained an error.The affiliation of the author Ray A.Bressan should be below:4.Department of Horticulture and Landscape Architecture,Purdue University,West Lafayette,IN 47907,USA.The author Ray A.Bressan’s affiliation has been updated in this Correction,and the original article has been updated as well.展开更多
基金supported by the Chinese Academy of Sciences,the CAS Strategic Priority Research Program to J.-K.Z.(Grant No.XDB27040101)by the National Natural Science Foundation of China to X.Z.(Grant No.31872807).
文摘Much has been learned about how plants acclimate to stressful environments,but the molecular basis of stress adaptation and the potential involvement of epigenetic regulation remain poorly understood.Here,we examined if salt stress induces mutagenesis in suspension cultured plant cells and if DNA methylation affects the mutagenesis using whole genome resequencing analysis.We generated suspension cell cultures from two Arabidopsis DNA methylation-deficient mutants and wild-type plants,and subjected the cultured cells to stepwise increases in salt stress intensity over 40 culture cycles.We show that ddc(drm1 drm2 cmt3)mutant cells can adapt to grow in 175 mM NaCl-containing growth medium and exhibit higher adaptability compared to wild type Col-0 and nrpe1 cells,which can adapt to grow in only 125mM NaCl-containing growth medium.Salt treated nrpe1 and ddc cells but not wild type cells accumulate more mutations compared with their respective untreated cells.There is no enrichment of stress responsive genes in the list of mutated genes in salt treated cells compared to the list of mutated genes in untreated cells.Our results suggest that DNA methylation prevents the induction of mutagenesis by salt stress in plant cells during stress adaptation.
文摘Following publication of this article(Zhu et al.2021),it is noticed that the article contained an error.The affiliation of the author Ray A.Bressan should be below:4.Department of Horticulture and Landscape Architecture,Purdue University,West Lafayette,IN 47907,USA.The author Ray A.Bressan’s affiliation has been updated in this Correction,and the original article has been updated as well.
