Clinical trials have shown beneficial effects of probiotics on inflammatory bowel diseases (IBD), although the exact mechanism remains unknown. VSL#3, a mixture of 8 probiotic bacteria, has been confirmed to have adju...Clinical trials have shown beneficial effects of probiotics on inflammatory bowel diseases (IBD), although the exact mechanism remains unknown. VSL#3, a mixture of 8 probiotic bacteria, has been confirmed to have adjunctive therapeutic effects on colitis. T follicular helper (Tfh) cells, a new separate subset of CD4+ T helper cells, have been proved to play a vital role in autoimmunity. The present study aimed to identify the beneficial effect of the probiotic mixture VSL#3 on the mouse model of colitis by regulating Tfh cells. Dextran sulfate sodium (DSS) was used to induce chronic colitis in C57BL/6 mice. VSL#3 (3x109 live bacteria) was given to C57BL/6 mice every other day for 60 days by gavage. The disease activity index (DAI), histological activity index (HAI), colon length and myeloperoxidase (MPO) activity were detected. Immunofluorescence was used to visualize the location of Tfh cells. Immunoglobulins, Tfh cells and plasma cells were quantified by enzyme-linked immunosorbent assay (ELISA), flow cytometry, real-time PCR or Western blotting. The results showed that after DSS treatment, the humoral immunity was disordered in C57BL/6 mice, with increased IgM, IgG and IgA levels in colonic mucus and increased Tfh cells in mesenteric lymph nodes (MLN). VSL#3 treatment showed anti-inflammatory effects as evidenced by reduced DAI score, HAI score and MPO activity. IgM, IgG and IgA levels were significantly reduced in colon mucus, and the number of Tfh cells was markedly decreased in MLN after VSL#3 treatment. It was concluded that VSL#3 alleviates DSS-induced colitis by downregulating Tfh cells, and Tfh cells may become a potential therapeutic target for IBD.展开更多
BACKGROUND Most of the recent research on nightmare distress and depressive symptoms has focused on adolescents and students,with less research on the nurse population.Emergency department nurses are at high risk for ...BACKGROUND Most of the recent research on nightmare distress and depressive symptoms has focused on adolescents and students,with less research on the nurse population.Emergency department nurses are at high risk for nightmare distress and depressive symptoms,but no studies have been conducted to explore the relationship between the two;thus,further investigation is needed.AIM To understand the relationship between nightmare distress and depressive symptoms among emergency department nurses in China.METHODS A convenience sampling method was used to select 280 emergency department nurses from nine provinces,including Jiangxi,Sichuan,Jiangsu,and Shanxi Provinces.The Chinese version of the Nightmare Distress Questionnaire and the Center for Epidemiological Studies Depression Scale(CES-D)were administered.RESULTS Emergency department nurses’nightmare distress scores were positively associated with depressive symptom scores(r=0.732),depressed affect(r=0.727),somatic symptoms(r=0.737),and interpersonal difficulty(r=0.647).Further multiple linear regression analyses showed that education level,work pressure,self-reported health,and CES-D scores were factors that influenced nightmare distress among Chinese emergency department nurses(P<0.05).CONCLUSION Nightmare distress is closely associated with depressive symptoms in Chinese emergency department nurses,and early intervention is recommended for professionals with this type of sleep disorder to reduce the occurrence of depressive symptoms.展开更多
目的了解湖南省某儿童医院跌落伤住院患者在时间、人群以及跌落位置的分布特点,为跌落伤的预防策略提供依据。方法自湖南省某儿童医院的医院信息系统(Hospital Information System,HIS)中收集2010—2014年间确诊为跌落伤的患者有关信息...目的了解湖南省某儿童医院跌落伤住院患者在时间、人群以及跌落位置的分布特点,为跌落伤的预防策略提供依据。方法自湖南省某儿童医院的医院信息系统(Hospital Information System,HIS)中收集2010—2014年间确诊为跌落伤的患者有关信息,采用频数、构成比和增长速度对住院患者特征进行描述,组间构成比差异采用卡方检验进行比较。结果湖南省某儿童医院2010—2014年跌落伤病例2 207人,发生人次呈逐年增加的趋势;男性患者的比例高于女性,住院患者的年龄主要集中在1~6岁,6岁以上患者随着年龄增加住院人次减少,农村患者所占比例大于城镇患者;主要从同一水平面或高处跌落;年龄、住院季节、跌落位置、受伤部位、受伤性质对疾病转归有影响。结论应该根据跌落伤患者的住院特征,针对高危因素采取具体措施预防跌落伤的发生。展开更多
基金This study was supported by the National Natural Science Foundation of China (Nos.81800984,81170361).
文摘Clinical trials have shown beneficial effects of probiotics on inflammatory bowel diseases (IBD), although the exact mechanism remains unknown. VSL#3, a mixture of 8 probiotic bacteria, has been confirmed to have adjunctive therapeutic effects on colitis. T follicular helper (Tfh) cells, a new separate subset of CD4+ T helper cells, have been proved to play a vital role in autoimmunity. The present study aimed to identify the beneficial effect of the probiotic mixture VSL#3 on the mouse model of colitis by regulating Tfh cells. Dextran sulfate sodium (DSS) was used to induce chronic colitis in C57BL/6 mice. VSL#3 (3x109 live bacteria) was given to C57BL/6 mice every other day for 60 days by gavage. The disease activity index (DAI), histological activity index (HAI), colon length and myeloperoxidase (MPO) activity were detected. Immunofluorescence was used to visualize the location of Tfh cells. Immunoglobulins, Tfh cells and plasma cells were quantified by enzyme-linked immunosorbent assay (ELISA), flow cytometry, real-time PCR or Western blotting. The results showed that after DSS treatment, the humoral immunity was disordered in C57BL/6 mice, with increased IgM, IgG and IgA levels in colonic mucus and increased Tfh cells in mesenteric lymph nodes (MLN). VSL#3 treatment showed anti-inflammatory effects as evidenced by reduced DAI score, HAI score and MPO activity. IgM, IgG and IgA levels were significantly reduced in colon mucus, and the number of Tfh cells was markedly decreased in MLN after VSL#3 treatment. It was concluded that VSL#3 alleviates DSS-induced colitis by downregulating Tfh cells, and Tfh cells may become a potential therapeutic target for IBD.
基金The study was approved by the Ethics Committee of the First Affiliated Hospital of Nanchang University(Ethics number:IIT2023196).
文摘BACKGROUND Most of the recent research on nightmare distress and depressive symptoms has focused on adolescents and students,with less research on the nurse population.Emergency department nurses are at high risk for nightmare distress and depressive symptoms,but no studies have been conducted to explore the relationship between the two;thus,further investigation is needed.AIM To understand the relationship between nightmare distress and depressive symptoms among emergency department nurses in China.METHODS A convenience sampling method was used to select 280 emergency department nurses from nine provinces,including Jiangxi,Sichuan,Jiangsu,and Shanxi Provinces.The Chinese version of the Nightmare Distress Questionnaire and the Center for Epidemiological Studies Depression Scale(CES-D)were administered.RESULTS Emergency department nurses’nightmare distress scores were positively associated with depressive symptom scores(r=0.732),depressed affect(r=0.727),somatic symptoms(r=0.737),and interpersonal difficulty(r=0.647).Further multiple linear regression analyses showed that education level,work pressure,self-reported health,and CES-D scores were factors that influenced nightmare distress among Chinese emergency department nurses(P<0.05).CONCLUSION Nightmare distress is closely associated with depressive symptoms in Chinese emergency department nurses,and early intervention is recommended for professionals with this type of sleep disorder to reduce the occurrence of depressive symptoms.
文摘目的了解湖南省某儿童医院跌落伤住院患者在时间、人群以及跌落位置的分布特点,为跌落伤的预防策略提供依据。方法自湖南省某儿童医院的医院信息系统(Hospital Information System,HIS)中收集2010—2014年间确诊为跌落伤的患者有关信息,采用频数、构成比和增长速度对住院患者特征进行描述,组间构成比差异采用卡方检验进行比较。结果湖南省某儿童医院2010—2014年跌落伤病例2 207人,发生人次呈逐年增加的趋势;男性患者的比例高于女性,住院患者的年龄主要集中在1~6岁,6岁以上患者随着年龄增加住院人次减少,农村患者所占比例大于城镇患者;主要从同一水平面或高处跌落;年龄、住院季节、跌落位置、受伤部位、受伤性质对疾病转归有影响。结论应该根据跌落伤患者的住院特征,针对高危因素采取具体措施预防跌落伤的发生。
