Dragon’s blood(DB)has shown a protective effect on neurological diseases.Microgravity(MG)or simulated MG(SMG)can induce blood-brain barrier(BBB)dysfunction,which is a characteristic feature of neurological disorders....Dragon’s blood(DB)has shown a protective effect on neurological diseases.Microgravity(MG)or simulated MG(SMG)can induce blood-brain barrier(BBB)dysfunction,which is a characteristic feature of neurological disorders.This study’s purpose was to evaluate the effect of DB on SMG-induced BBB dysfunction and explore its signaling pathway.Both DB and vitamin C(Vc)were administered orally for tail-suspended rats within 3 weeks.DB and Vc solutions were added to human brain microvascular endothelial cells(HCMEC/D3)cells,which were then exposed to SMG for 24 h.The protective effect of DB was assessed by hematoxylin and eosin and Nissl staining,ultrastructure observation,and permeability in rats.Cell apoptosis and the distribution of tight junction(TJ)and adherens junction(AJ)proteins and filamentous actin(F-actin)were examined in HCMEC/D3.The oxidative stress and inflammation,and TJ and AJ protein expressions were determined in rat brain and HCMEC/D3.The focal adhesion kinase(FAK)signaling pathway proteins were determined.DB protected SMG-induced rat BBB disruption by improving neuronal apoptosis,repairing widened intercellular space,and decreasing BBB permeability.DB effectively relieved SMG-induced HCMEC/D3 damage by inhibiting cell apoptosis and restoring F-actin spindle distribution.High doses of DB upregulated TJ and AJ protein expressions and decreased oxidative stress and proinflammatory cytokine levels in rat brain and HCMEC/D3.DB enhanced the expressions of FAK signal transduction proteins and F-actin/globular actin(G-actin)ratio in rat brain and HCMEC/D3,suggesting that DB promotes actin cytoskeleton polymerization,benefits the endothelial cell-cell and cell-extracellular matrix adhesion,and,in consequence,contributes to BBB integrity.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.81973572)the Space Health Research Foundation of Astronaut Health Center(Grant No.AHCC2021KF004).
文摘Dragon’s blood(DB)has shown a protective effect on neurological diseases.Microgravity(MG)or simulated MG(SMG)can induce blood-brain barrier(BBB)dysfunction,which is a characteristic feature of neurological disorders.This study’s purpose was to evaluate the effect of DB on SMG-induced BBB dysfunction and explore its signaling pathway.Both DB and vitamin C(Vc)were administered orally for tail-suspended rats within 3 weeks.DB and Vc solutions were added to human brain microvascular endothelial cells(HCMEC/D3)cells,which were then exposed to SMG for 24 h.The protective effect of DB was assessed by hematoxylin and eosin and Nissl staining,ultrastructure observation,and permeability in rats.Cell apoptosis and the distribution of tight junction(TJ)and adherens junction(AJ)proteins and filamentous actin(F-actin)were examined in HCMEC/D3.The oxidative stress and inflammation,and TJ and AJ protein expressions were determined in rat brain and HCMEC/D3.The focal adhesion kinase(FAK)signaling pathway proteins were determined.DB protected SMG-induced rat BBB disruption by improving neuronal apoptosis,repairing widened intercellular space,and decreasing BBB permeability.DB effectively relieved SMG-induced HCMEC/D3 damage by inhibiting cell apoptosis and restoring F-actin spindle distribution.High doses of DB upregulated TJ and AJ protein expressions and decreased oxidative stress and proinflammatory cytokine levels in rat brain and HCMEC/D3.DB enhanced the expressions of FAK signal transduction proteins and F-actin/globular actin(G-actin)ratio in rat brain and HCMEC/D3,suggesting that DB promotes actin cytoskeleton polymerization,benefits the endothelial cell-cell and cell-extracellular matrix adhesion,and,in consequence,contributes to BBB integrity.
