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Identification of circulating CD90^+ CD73^+ cells in cirrhosis of liver
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作者 Mitnala Sasikala Pugazhelthi Surya +5 位作者 Gaddipati Radhika Pondugala Pavan Kumar Mekala Subba Rao rathindra mohan mukherjee Padaki Nagaraja Rao D Nageshwar Reddy 《World Journal of Stem Cells》 SCIE CAS 2011年第7期63-69,共7页
AIM:To identify circulating CD90 + CD73 + CD45 cells and evaluate their in vitro proliferating abilities.METHODS:Patients with cirrhosis(n=43),and healthy volunteers(n=40)were recruited to the study.Mononuclear cells ... AIM:To identify circulating CD90 + CD73 + CD45 cells and evaluate their in vitro proliferating abilities.METHODS:Patients with cirrhosis(n=43),and healthy volunteers(n=40)were recruited to the study.Mononuclear cells were isolated and cultured from the peripheral blood of controls and cirrhosis patients.Fibroblast-like cells that appeared in cultures were analyzed for morphological features,enumerated by flow cytometry and confirmed by immunocytochemistry(ICC).Colony forming efficiency(CFE)of these cells was assessed and expressed as a percentage.RESULTS:In comparison to healthy volunteers,cells obtained from cirrhotic patients showed a significantincrease(P<0.001)in the percentage of CD90+CD73+ CD45 cells in culture.Cultured cells also showed 10 fold increases in CFE.Flow cytometry and ICC confirmed that the proliferating cells expressed CD90 + CD73 + in the cultures from cirrhosis patients.CONCLUSION:These results indicate the presence of circulating CD90 + CD73 + CD45 cells in patients with liver cirrhosis that have the potential to proliferate at a higher rate. 展开更多
关键词 CD90+ CD73+ CD45 CELLS LIVER CIRRHOSIS Proliferation Colony forming efficiency
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