The suspected cases of PRRSV in a pig farm of Shandong Province were conducted pathologic autopsy, RT-PCR amplification, and sequencing and analysis of PRRSV N protein gene. The results showed that the homology of the...The suspected cases of PRRSV in a pig farm of Shandong Province were conducted pathologic autopsy, RT-PCR amplification, and sequencing and analysis of PRRSV N protein gene. The results showed that the homology of the nucleotide sequences of protein N was 99.7% between the isolate and the classical strains (VR2332 and SD1), and 93.4% between the isolate and the highly pathogenic strains (JXA1, HuN4, SX-1 and TJM). The results suggested that the virus was classical PRRSV in the pig farm. The research would provide a reference for the diagnosis and targeted therapy of classical PRRSV infection in swine production.展开更多
[Objective] To identify suspected cases of highly pathogenic PRRSV from a pig farm. [Method] The suspected cases of highly pathogenic PRRSV were conducted pathologic anatomy, the gene of PRRSV N protein was amplified ...[Objective] To identify suspected cases of highly pathogenic PRRSV from a pig farm. [Method] The suspected cases of highly pathogenic PRRSV were conducted pathologic anatomy, the gene of PRRSV N protein was amplified by RT-PCR and its sequences were determined and analyzed. [Result] The nucleotide sequence of N protein of N7/N8 isolate shared the consistency of 97.0% with highly pathogenic strains （JXA1, HUN4, SX-1 and TJ）, and that of isolate N9 shared the consistency of 97.3% with highly pathogenic strains, indicating the infected virus in the pig farm was highly pathogenic PRRSV. [Conclusion] This research provides a reference for the diagnosis of highly pathogenic PRRSV infec- tion in swine production.展开更多
基金Supported by National Natural Science Foundation of China(31572550)Shandong Provincial Mod-ern Agricultural Industry and Technology System(SDAIT)+1 种基金Agricultural Science and Technology In-novation Projects of Shandong Academy of Agricultural Sciences(CXGC2016B14,CXGC2018E11,CXGC2018E10)Taishan Scholar Project
文摘The suspected cases of PRRSV in a pig farm of Shandong Province were conducted pathologic autopsy, RT-PCR amplification, and sequencing and analysis of PRRSV N protein gene. The results showed that the homology of the nucleotide sequences of protein N was 99.7% between the isolate and the classical strains (VR2332 and SD1), and 93.4% between the isolate and the highly pathogenic strains (JXA1, HuN4, SX-1 and TJM). The results suggested that the virus was classical PRRSV in the pig farm. The research would provide a reference for the diagnosis and targeted therapy of classical PRRSV infection in swine production.
基金Supported by National Natural Science Foundation of China(31572550)Agricultural Industry Re-search System of Shandong Province(SDAIT-06-022-06)+1 种基金Major Scientific and Technological Achievements Training Program of Shandong Academy of Agricultural Sciences(2014CGPY04)Project of Taishan Scholar
文摘[Objective] To identify suspected cases of highly pathogenic PRRSV from a pig farm. [Method] The suspected cases of highly pathogenic PRRSV were conducted pathologic anatomy, the gene of PRRSV N protein was amplified by RT-PCR and its sequences were determined and analyzed. [Result] The nucleotide sequence of N protein of N7/N8 isolate shared the consistency of 97.0% with highly pathogenic strains （JXA1, HUN4, SX-1 and TJ）, and that of isolate N9 shared the consistency of 97.3% with highly pathogenic strains, indicating the infected virus in the pig farm was highly pathogenic PRRSV. [Conclusion] This research provides a reference for the diagnosis of highly pathogenic PRRSV infec- tion in swine production.
