目的:探究异氟醚对人结肠癌S W480细胞增殖、凋亡、侵袭和迁移的影响及其分子机制。方法:SW480细胞随机分为对照组(SW480)、异氟醚(1.5%,2.0%,2.5%)组,共4组。噻唑蓝比色法(MTT)检测细胞增殖,Hoechst染色检测细胞凋亡,Transwell检测细...目的:探究异氟醚对人结肠癌S W480细胞增殖、凋亡、侵袭和迁移的影响及其分子机制。方法:SW480细胞随机分为对照组(SW480)、异氟醚(1.5%,2.0%,2.5%)组,共4组。噻唑蓝比色法(MTT)检测细胞增殖,Hoechst染色检测细胞凋亡,Transwell检测细胞侵袭,划痕试验分析细胞迁移,Western印迹法检测细胞增殖核抗原67(antigen identified by monoclonal antibody,Ki-67),Caspase-3,LC3II,LC3I,血管内皮细胞生长因子(vascular endothelial grow th factor,VEGF),磷脂酰肌醇3-激酶(phosphatidylinositide 3-kinases,PI3K),p-PI3K,蛋白激酶B(protein kinase B,A K T)和p-A K T蛋白水平。结果:与对照组相比,异氟醚(1.5%,2.0%,2.5%)组细胞增殖倍数下降(P=0.0093,n=5)。异氟醚(1.5%,2%,2.5%)组细胞凋亡率高于对照组(P=0.0087,n=5)。而且,异氟醚(1.5%,2.0%,2.5%)组每个视野下的侵袭细胞数低于对照组(P=0.0081,n=5)。与对照组相比,异氟醚(1.5%,2.0%,2.5%)组划痕愈合率降低(P=0.0078,n=5)。异氟醚(1.5%,2.0%,2.5%)组K i-6 7和V EG F及L C 3 I I/L C 3 I的比值表达低于对照组,C a s p a s e-3表达高于对照组(P=0.0096,n=5)。另外,异氟醚(1.5%,2.0%,2.5%)组p-PI3K/PI3K和p-AKT/AKT比值低于对照组(P=0.0099,n=5)。IGF-1可逆转异氟醚诱导的p-PI3K,p-AKT,Ki-67及VEGF蛋白水平升高和Caspase的蛋白水平下降(P=0.0079,n=5)。结论:异氟醚可通过抑制PI3K/AKT信号通路活化减弱人结肠癌细胞SW480细胞增殖、侵袭和迁移,促进细胞凋亡。展开更多
On the basis of an analysis of data from the Chinese Social Survey(CSS)2015,and with male social participation as the reference,we undertook an empirical study of Chinese women’s current social participation and the ...On the basis of an analysis of data from the Chinese Social Survey(CSS)2015,and with male social participation as the reference,we undertook an empirical study of Chinese women’s current social participation and the factors affecting it along the two dimensions of“marriage and family life”and“employment and income status,”as well as the basic characteristics of individuals.Our findings show that in general women display a lower level of social participation than men.In terms of type of social participation,women are more likely to take part in policy-supported activities or serve as volunteers for public interest activities but less likely to participate in the activities of particular“action organizations”or the expression of opinion.Women’s social participation is affected by many factors,including basic individual characteristics,marital status and family life,and employment and income status.A further gender comparison finds that female social participation tends to be more affected by marriage and family life,whereas male social participation is more closely related to employment and income status.We conclude that although more Chinese women have stepped out of the bonds of“home and family,”their social participation“outside the family”is family-bound in a way that is quite different that of men.Hence if we are to pursue gender equality at the level of social participation,it remains necessary to give women greater assurances in terms of marriage and family life by encouraging the realization of their self-worth and facilitating their social development,so as to coordinate and alleviate the double pressures of family and work,thus making women’s social participation an important force and enhancing their all-round development.展开更多
文摘目的:探究异氟醚对人结肠癌S W480细胞增殖、凋亡、侵袭和迁移的影响及其分子机制。方法:SW480细胞随机分为对照组(SW480)、异氟醚(1.5%,2.0%,2.5%)组,共4组。噻唑蓝比色法(MTT)检测细胞增殖,Hoechst染色检测细胞凋亡,Transwell检测细胞侵袭,划痕试验分析细胞迁移,Western印迹法检测细胞增殖核抗原67(antigen identified by monoclonal antibody,Ki-67),Caspase-3,LC3II,LC3I,血管内皮细胞生长因子(vascular endothelial grow th factor,VEGF),磷脂酰肌醇3-激酶(phosphatidylinositide 3-kinases,PI3K),p-PI3K,蛋白激酶B(protein kinase B,A K T)和p-A K T蛋白水平。结果:与对照组相比,异氟醚(1.5%,2.0%,2.5%)组细胞增殖倍数下降(P=0.0093,n=5)。异氟醚(1.5%,2%,2.5%)组细胞凋亡率高于对照组(P=0.0087,n=5)。而且,异氟醚(1.5%,2.0%,2.5%)组每个视野下的侵袭细胞数低于对照组(P=0.0081,n=5)。与对照组相比,异氟醚(1.5%,2.0%,2.5%)组划痕愈合率降低(P=0.0078,n=5)。异氟醚(1.5%,2.0%,2.5%)组K i-6 7和V EG F及L C 3 I I/L C 3 I的比值表达低于对照组,C a s p a s e-3表达高于对照组(P=0.0096,n=5)。另外,异氟醚(1.5%,2.0%,2.5%)组p-PI3K/PI3K和p-AKT/AKT比值低于对照组(P=0.0099,n=5)。IGF-1可逆转异氟醚诱导的p-PI3K,p-AKT,Ki-67及VEGF蛋白水平升高和Caspase的蛋白水平下降(P=0.0079,n=5)。结论:异氟醚可通过抑制PI3K/AKT信号通路活化减弱人结肠癌细胞SW480细胞增殖、侵袭和迁移,促进细胞凋亡。
文摘On the basis of an analysis of data from the Chinese Social Survey(CSS)2015,and with male social participation as the reference,we undertook an empirical study of Chinese women’s current social participation and the factors affecting it along the two dimensions of“marriage and family life”and“employment and income status,”as well as the basic characteristics of individuals.Our findings show that in general women display a lower level of social participation than men.In terms of type of social participation,women are more likely to take part in policy-supported activities or serve as volunteers for public interest activities but less likely to participate in the activities of particular“action organizations”or the expression of opinion.Women’s social participation is affected by many factors,including basic individual characteristics,marital status and family life,and employment and income status.A further gender comparison finds that female social participation tends to be more affected by marriage and family life,whereas male social participation is more closely related to employment and income status.We conclude that although more Chinese women have stepped out of the bonds of“home and family,”their social participation“outside the family”is family-bound in a way that is quite different that of men.Hence if we are to pursue gender equality at the level of social participation,it remains necessary to give women greater assurances in terms of marriage and family life by encouraging the realization of their self-worth and facilitating their social development,so as to coordinate and alleviate the double pressures of family and work,thus making women’s social participation an important force and enhancing their all-round development.