At present, Brazil imports approximately 11 billion liters/year of diesel. With the interruption of the works in the new Petrobras refineries, the projection is that by 2025 this volume will increase to 24.2 billion l...At present, Brazil imports approximately 11 billion liters/year of diesel. With the interruption of the works in the new Petrobras refineries, the projection is that by 2025 this volume will increase to 24.2 billion liters of diesel/year. In this sense, the biodiesel factory Grand Valle Bio Energy Ltda., located in the state of Rio de Janeiro, in conjunction with the FAPERJ makes some investments in technology development for the cultivation and use of microalgae as an alternative raw material in the production of biodiesel. Based on arguments previously said, this work presents the results of the microalgae cultivation Monoraphidium sp. in photobioreactors the pilot plant of the company. The installation with an area of 120 m2 is included with 2 open photobioreactors of type falling film (20 m × 1 m), with a cascade of 18mm and capacity of 4000 L. The lineage cultivated is selected from previous ecophysiological studies that are identified as promising for biodiesel production by having a high potential for the production of lipids. This lineage is maintained at collection of the stock of cultures Laboratory of Green Technologies of the School of Chemistry/ UFRJ. The cultivation was performed in means ASM-1 (Gorham et al., 1964), initial pH 8.0, with aeration and circulation average of 8 hours a day during 19 days. The culture was started with an inoculum of 1 × 107 cel/ml. The lipid production was determined in two phases of growth: on day 4 (exponential phase) and 15 day (stationary phase). For the determination and quantification of lipid content, two different methods were assessed for a sample of biomass, submitted to the same processes the separation and drying. The results showed the methodology of Bligh & Dyer with modifications as the most efficient in extracting lipids. The total lipid content of the biomass Monoraphidium sp. was 30.58%. The growth rate varied between 0.74 ± 0.01 and 0.68 ± 0.02.展开更多
This work studies the saponification which directs the wet biomass of algae Chlamydomonas sp. like a previous stage to production of biodiesel. This stage allows the obtainment of fatty acids to produce biodiesel, ins...This work studies the saponification which directs the wet biomass of algae Chlamydomonas sp. like a previous stage to production of biodiesel. This stage allows the obtainment of fatty acids to produce biodiesel, instead of the gross lipid fraction. In addition of the fatty acids, utilizing the same process one can also obtain the fraction unsaponifiable, these are soluble in apolar solvents and contain mainly carotenoids that can take action as antioxidants and photoprotectors, as they reduce the oxidation of unsaturated fatty acids. The saponification direct and extraction of fatty acids from biomass is faster and reduces the time and cost of operation. The separation of unsaponifiable matter from the biomass humid of microalgae Chlamydomonas sp., was held according to the method AOCS (Ca 6a-40), using extraction Liquid-liquid with hexane as solvent. Subsequently, phase hydroalcoholic or from soap, containing fatty acids, was acidified by addition of H2SO4 and the fatty acids were recovered by the addition of hexane. After acidulation of the soap, necessary for obtaining of the fatty acids was performed the stage of esterification for obtaining of biodiesel. The operating conditions were: molar ratio fatty acid:methanol (1:10), as catalyst 8% H2SO4 calculated in relation to the mass of fatty acid, 200℃ and reaction time of 90 minutes. The content of methyl esters was 96.8% determined by gas chromatography according to standard EN14103. The quality of biodiesel produced from wet biomass of Chlamydomonas sp. is according to the specification established by standard EN 14214 and RANP No. 14. For the identification of the composition the unsaponifiable fraction was used the method of High Performance Liquid Chromatography (HPLC). The composition of the material unsaponifiable found was of: Carotenoids total (0.76%);Lutein (0.45%);Zeaxanthin (0.07%);α-carotene (0.05%);β-carotene (0.11%);13 cisβ-carotene (0.05%) and 9-cisβ-carotene (0.03%).展开更多
文摘At present, Brazil imports approximately 11 billion liters/year of diesel. With the interruption of the works in the new Petrobras refineries, the projection is that by 2025 this volume will increase to 24.2 billion liters of diesel/year. In this sense, the biodiesel factory Grand Valle Bio Energy Ltda., located in the state of Rio de Janeiro, in conjunction with the FAPERJ makes some investments in technology development for the cultivation and use of microalgae as an alternative raw material in the production of biodiesel. Based on arguments previously said, this work presents the results of the microalgae cultivation Monoraphidium sp. in photobioreactors the pilot plant of the company. The installation with an area of 120 m2 is included with 2 open photobioreactors of type falling film (20 m × 1 m), with a cascade of 18mm and capacity of 4000 L. The lineage cultivated is selected from previous ecophysiological studies that are identified as promising for biodiesel production by having a high potential for the production of lipids. This lineage is maintained at collection of the stock of cultures Laboratory of Green Technologies of the School of Chemistry/ UFRJ. The cultivation was performed in means ASM-1 (Gorham et al., 1964), initial pH 8.0, with aeration and circulation average of 8 hours a day during 19 days. The culture was started with an inoculum of 1 × 107 cel/ml. The lipid production was determined in two phases of growth: on day 4 (exponential phase) and 15 day (stationary phase). For the determination and quantification of lipid content, two different methods were assessed for a sample of biomass, submitted to the same processes the separation and drying. The results showed the methodology of Bligh & Dyer with modifications as the most efficient in extracting lipids. The total lipid content of the biomass Monoraphidium sp. was 30.58%. The growth rate varied between 0.74 ± 0.01 and 0.68 ± 0.02.
文摘This work studies the saponification which directs the wet biomass of algae Chlamydomonas sp. like a previous stage to production of biodiesel. This stage allows the obtainment of fatty acids to produce biodiesel, instead of the gross lipid fraction. In addition of the fatty acids, utilizing the same process one can also obtain the fraction unsaponifiable, these are soluble in apolar solvents and contain mainly carotenoids that can take action as antioxidants and photoprotectors, as they reduce the oxidation of unsaturated fatty acids. The saponification direct and extraction of fatty acids from biomass is faster and reduces the time and cost of operation. The separation of unsaponifiable matter from the biomass humid of microalgae Chlamydomonas sp., was held according to the method AOCS (Ca 6a-40), using extraction Liquid-liquid with hexane as solvent. Subsequently, phase hydroalcoholic or from soap, containing fatty acids, was acidified by addition of H2SO4 and the fatty acids were recovered by the addition of hexane. After acidulation of the soap, necessary for obtaining of the fatty acids was performed the stage of esterification for obtaining of biodiesel. The operating conditions were: molar ratio fatty acid:methanol (1:10), as catalyst 8% H2SO4 calculated in relation to the mass of fatty acid, 200℃ and reaction time of 90 minutes. The content of methyl esters was 96.8% determined by gas chromatography according to standard EN14103. The quality of biodiesel produced from wet biomass of Chlamydomonas sp. is according to the specification established by standard EN 14214 and RANP No. 14. For the identification of the composition the unsaponifiable fraction was used the method of High Performance Liquid Chromatography (HPLC). The composition of the material unsaponifiable found was of: Carotenoids total (0.76%);Lutein (0.45%);Zeaxanthin (0.07%);α-carotene (0.05%);β-carotene (0.11%);13 cisβ-carotene (0.05%) and 9-cisβ-carotene (0.03%).
