The efficacy of intraperitoneal GY Y4137 therapy and intratesticular GY Y4137 therapy in an experimental rat model was investigated.Four groups were set up as the sham-operation group,torsion/detorsion(T/D)group,T/D p...The efficacy of intraperitoneal GY Y4137 therapy and intratesticular GY Y4137 therapy in an experimental rat model was investigated.Four groups were set up as the sham-operation group,torsion/detorsion(T/D)group,T/D plus intraperitoneal GY Y4137(G-IP)group,and T/D plus intratesticular GY Y4137(G-IT)group.In order to establish a testicular T/D model,the left testis was opcrated and the rotation reached 720°clockwise which lasted 1 h before reperfiusion.The G-IP group accepted 100 umol/kg of GY Y4137 intraperitoneally 30 min after testicular rotation,while the G-IT group was treated with the same dose by intratesticular injection.Six h after detorsion,the testis was collcted and subsequently asssed.The T/D group showed signifcant changes in histology and an enhancement in the level of oxidative stress and apoptosis compared to the sham-operation group.The expression of Caspase-3 and Bax turmed out to be strengthened by T/D and relatively decreased with GY Y4137 treatment in both the G-IP and G-IT groups.Moreover,the Bcl-2 expression was inhibited in the T/D group,and promoted by GYY4137 in the G-IP and G-IT groups.GYY4137,moderating these observed changes,displayed a more protective cffect with G-IT therapy than G-IP therapy.This study indicated that the efficacy of intratesticular therapy with GYY4137 is better than that of intraperitoneal therapy,which may provide a more valuable approach for testicular torsion therapy.展开更多
文摘The efficacy of intraperitoneal GY Y4137 therapy and intratesticular GY Y4137 therapy in an experimental rat model was investigated.Four groups were set up as the sham-operation group,torsion/detorsion(T/D)group,T/D plus intraperitoneal GY Y4137(G-IP)group,and T/D plus intratesticular GY Y4137(G-IT)group.In order to establish a testicular T/D model,the left testis was opcrated and the rotation reached 720°clockwise which lasted 1 h before reperfiusion.The G-IP group accepted 100 umol/kg of GY Y4137 intraperitoneally 30 min after testicular rotation,while the G-IT group was treated with the same dose by intratesticular injection.Six h after detorsion,the testis was collcted and subsequently asssed.The T/D group showed signifcant changes in histology and an enhancement in the level of oxidative stress and apoptosis compared to the sham-operation group.The expression of Caspase-3 and Bax turmed out to be strengthened by T/D and relatively decreased with GY Y4137 treatment in both the G-IP and G-IT groups.Moreover,the Bcl-2 expression was inhibited in the T/D group,and promoted by GYY4137 in the G-IP and G-IT groups.GYY4137,moderating these observed changes,displayed a more protective cffect with G-IT therapy than G-IP therapy.This study indicated that the efficacy of intratesticular therapy with GYY4137 is better than that of intraperitoneal therapy,which may provide a more valuable approach for testicular torsion therapy.
