Iron(Fe)is essential for DNA synthesis,photosynthesis and respiration of plants.The demand for Fe substantially increases during legumesrhizobia symbiotic nitrogen fixation because of the synthesis of leghemoglobin in...Iron(Fe)is essential for DNA synthesis,photosynthesis and respiration of plants.The demand for Fe substantially increases during legumesrhizobia symbiotic nitrogen fixation because of the synthesis of leghemoglobin in the host and Fecontaining proteins in bacteroids.However,the mechanism by which plant controls iron transport to nodules remains largely unknown.Here we demonstrate that GmYSL7 serves as a key regulator controlling Fe uptake from root to nodule and distribution in soybean nodules.GmYSL7 is Fe responsive and GmYSL7 transports iron across the membrane and into the infected cells of nodules.Alterations of GmYSL7 substantially affect iron distribution between root and nodule,resulting in defective growth of nodules and reduced nitrogenase activity.GmYSL7 knockout increases the expression of GmbHLH300,a transcription factor required for Fe response of nodules.Overexpression of GmbHLH300 decreases nodule number,nitrogenase activity and Fe content in nodules.Remarkably,GmbHLH300 directly binds to the promoters of ENOD93 and GmLbs,which regulate nodule number and nitrogenase activity,and represses their transcription.Our data reveal a new role of GmYSL7 in controlling Fe transport from host root to nodule and Fe distribution in nodule cells,and uncover a molecular mechanism by which Fe affects nodule number and nitrogenase activity.展开更多
基金supported from the National Key Research and Development Program of China (2019YFA0904703)the Laboratory of Lingnan Modern Agriculture Project (NZ2021013)+3 种基金the National Natural Science Foundation of China (31730066,31961133029)the Fundamental Research Funds for the Central Universities (2021ZKPY012)the Natural Science Foundation of Hubei Province (2020CFA008)the Huazhong Agricultural University’s Scientific and Technological Self-innovation Foundation (2015RC014)。
文摘Iron(Fe)is essential for DNA synthesis,photosynthesis and respiration of plants.The demand for Fe substantially increases during legumesrhizobia symbiotic nitrogen fixation because of the synthesis of leghemoglobin in the host and Fecontaining proteins in bacteroids.However,the mechanism by which plant controls iron transport to nodules remains largely unknown.Here we demonstrate that GmYSL7 serves as a key regulator controlling Fe uptake from root to nodule and distribution in soybean nodules.GmYSL7 is Fe responsive and GmYSL7 transports iron across the membrane and into the infected cells of nodules.Alterations of GmYSL7 substantially affect iron distribution between root and nodule,resulting in defective growth of nodules and reduced nitrogenase activity.GmYSL7 knockout increases the expression of GmbHLH300,a transcription factor required for Fe response of nodules.Overexpression of GmbHLH300 decreases nodule number,nitrogenase activity and Fe content in nodules.Remarkably,GmbHLH300 directly binds to the promoters of ENOD93 and GmLbs,which regulate nodule number and nitrogenase activity,and represses their transcription.Our data reveal a new role of GmYSL7 in controlling Fe transport from host root to nodule and Fe distribution in nodule cells,and uncover a molecular mechanism by which Fe affects nodule number and nitrogenase activity.
