SDS-PAGE was applied to determine trypsin activity and inhibition. After the hydrolysis by trypsin to substrate bovine serum albulnin (BSA) under different temperatures and pH, the hydrolysis degree of BSA was conduct...SDS-PAGE was applied to determine trypsin activity and inhibition. After the hydrolysis by trypsin to substrate bovine serum albulnin (BSA) under different temperatures and pH, the hydrolysis degree of BSA was conducted using SDS-PAGE. From the quantitative analysis to the electrophoresis bands of BSA and its hydrolysis products in SDS-PAGE pattern, the change of trypsin activity was determined, and then the optimum temperature at 40°C and the optimum pH at pH 8.5 - 8.7 for trypsin activity were obtained. All the target bonds in BSA molecule could be hydrolyzed at the same time by trypsin. The inhibition was due to the binding of inhibitor to trypsin, which made it impossible for trypsin to touch the substrate protein. SDS-PAGE was demonstrated to be also an effect method for assaying the characteristics of trypsin activity and its inhibition.展开更多
Climate change will cause range shifts of many species in the future.Galliformes might be particularly vulnerable to climate change,as they have low dispersal ability.Little is known about their possible responses to ...Climate change will cause range shifts of many species in the future.Galliformes might be particularly vulnerable to climate change,as they have low dispersal ability.Little is known about their possible responses to the future climate.We used a generalized additive model to predict the current and future ranges of all 63 Galliformes in China,based on a comprehensive species occurrence database and a combination of climate variables.Other environmental variables(e.g.elevation and human footprint index)were also considered,as well as the latitude and longitude of the occurrences.Principal component analysis was conducted to illustrate the association between environmental variables and Galliformes distributions.Using the Special Report on Emissions Scenarios(SRES)A2 climate change scenario for 2071–2100,we projected that 29 species would have range shifts over 50%,including 13 endemic species.Galliformes at higher elevation face greater range shifts.Northward shifts are greater than those in other directions.We suggest conservationists pay special attention to the 29 Galliformes that face extensive range shifts,especially the endemic species among them.展开更多
文摘SDS-PAGE was applied to determine trypsin activity and inhibition. After the hydrolysis by trypsin to substrate bovine serum albulnin (BSA) under different temperatures and pH, the hydrolysis degree of BSA was conducted using SDS-PAGE. From the quantitative analysis to the electrophoresis bands of BSA and its hydrolysis products in SDS-PAGE pattern, the change of trypsin activity was determined, and then the optimum temperature at 40°C and the optimum pH at pH 8.5 - 8.7 for trypsin activity were obtained. All the target bonds in BSA molecule could be hydrolyzed at the same time by trypsin. The inhibition was due to the binding of inhibitor to trypsin, which made it impossible for trypsin to touch the substrate protein. SDS-PAGE was demonstrated to be also an effect method for assaying the characteristics of trypsin activity and its inhibition.
基金the ISZS international research program Biological Consequences of Global Change(BCGC)sponsored by Bureau of International Cooperation,Chinese Academy of Sciences(GJHZ200810).
文摘Climate change will cause range shifts of many species in the future.Galliformes might be particularly vulnerable to climate change,as they have low dispersal ability.Little is known about their possible responses to the future climate.We used a generalized additive model to predict the current and future ranges of all 63 Galliformes in China,based on a comprehensive species occurrence database and a combination of climate variables.Other environmental variables(e.g.elevation and human footprint index)were also considered,as well as the latitude and longitude of the occurrences.Principal component analysis was conducted to illustrate the association between environmental variables and Galliformes distributions.Using the Special Report on Emissions Scenarios(SRES)A2 climate change scenario for 2071–2100,we projected that 29 species would have range shifts over 50%,including 13 endemic species.Galliformes at higher elevation face greater range shifts.Northward shifts are greater than those in other directions.We suggest conservationists pay special attention to the 29 Galliformes that face extensive range shifts,especially the endemic species among them.
