Objective:Studies have shown that both short-term and long-term cold exposures disturb the biological process.The aim of the present study is to investigate the effects of intermittent cold exposure on proteomic profi...Objective:Studies have shown that both short-term and long-term cold exposures disturb the biological process.The aim of the present study is to investigate the effects of intermittent cold exposure on proteomic profiles in the hypothalamus and pituitary of female Sprague-Dawley(SD)rats.Materials and methods:The rats were exposed to-10°C in a cabin for 4 h per day,and the treatment lasted for 14 days.The comparative label-free LC-MS/MS analysis was performed to investigate the changes of proteomic profiles in the hypothalamus and pituitary.ELISA analysis was used to validate the expression of differential proteins.Results:22 differential proteins in the hypothalamus and 75 differential proteins in the pituitary were identified by the label-free proteomic analysis.Gene ontology annotation and enrichment analysis indicated that cold exposure disrupted protein phosphorylation,filopodium assembly,intracellular protein transport,peripheral nervous system neuron axonogenesis,spinal cord development,Golgi organization,positive regulation of pseudopodium assembly,and cell-cell adhesion.Three proteins(Cdc42,Ptprs,and Setd7)were down-regulated in the cold exposure group.Conclusion:The results indicate that intermittent cold exposure alters the proteomic profiles of hypothalamus and pituitary in female rats.展开更多
The dawn of the new millennium witnessed the rise of Internet literature in China. Time-travel historical romances （chuanyue lishi xiaoshuo） became one of the most prominent genres produced and circulated online. At...The dawn of the new millennium witnessed the rise of Internet literature in China. Time-travel historical romances （chuanyue lishi xiaoshuo） became one of the most prominent genres produced and circulated online. At the heart of each story lies a fictitious hero who goes back in time to witness, facilitate, delay or alter a particular historical incident or series of historical events. Focusing on temporal poetics, politics and ethics in Tong Hua＇s Startling by Each Step （Bubu ]ingxin） and its TV and film adaptations, whilst also drawing upon a number of other well-acclaimed time-travel historical romances, this paper builds a threefold argument. Firstly, I argue that the aesthetic fascination of this genre mainly consists in its dramatization of the temporal clash between imperial and modern zeitgeists, interweaving the traditional device of dreams to deal with the Lacanian Real; secondly, although the immense popularity of the time-travel motif evinces a fascination with the fast pace of the digital age, Startling by Each Step tempers that trend by slowing things down and suggesting a yearning for the individual＇s temporal sovereignty; finally, the causal loop innate in this genre necessitates a transgression of historical linearity which often leads to unintended catastrophes and results in a prevailing sense of historical determinism. However, in Startling by Each Step a Heidegger-like resolute struggle for finite freedom within time delivers from the recognition of one＇s fateful destiny.展开更多
基金the grants of Tianjin Institute of Environmental and Operational Medicine(BWS17J025).
文摘Objective:Studies have shown that both short-term and long-term cold exposures disturb the biological process.The aim of the present study is to investigate the effects of intermittent cold exposure on proteomic profiles in the hypothalamus and pituitary of female Sprague-Dawley(SD)rats.Materials and methods:The rats were exposed to-10°C in a cabin for 4 h per day,and the treatment lasted for 14 days.The comparative label-free LC-MS/MS analysis was performed to investigate the changes of proteomic profiles in the hypothalamus and pituitary.ELISA analysis was used to validate the expression of differential proteins.Results:22 differential proteins in the hypothalamus and 75 differential proteins in the pituitary were identified by the label-free proteomic analysis.Gene ontology annotation and enrichment analysis indicated that cold exposure disrupted protein phosphorylation,filopodium assembly,intracellular protein transport,peripheral nervous system neuron axonogenesis,spinal cord development,Golgi organization,positive regulation of pseudopodium assembly,and cell-cell adhesion.Three proteins(Cdc42,Ptprs,and Setd7)were down-regulated in the cold exposure group.Conclusion:The results indicate that intermittent cold exposure alters the proteomic profiles of hypothalamus and pituitary in female rats.
文摘The dawn of the new millennium witnessed the rise of Internet literature in China. Time-travel historical romances （chuanyue lishi xiaoshuo） became one of the most prominent genres produced and circulated online. At the heart of each story lies a fictitious hero who goes back in time to witness, facilitate, delay or alter a particular historical incident or series of historical events. Focusing on temporal poetics, politics and ethics in Tong Hua＇s Startling by Each Step （Bubu ]ingxin） and its TV and film adaptations, whilst also drawing upon a number of other well-acclaimed time-travel historical romances, this paper builds a threefold argument. Firstly, I argue that the aesthetic fascination of this genre mainly consists in its dramatization of the temporal clash between imperial and modern zeitgeists, interweaving the traditional device of dreams to deal with the Lacanian Real; secondly, although the immense popularity of the time-travel motif evinces a fascination with the fast pace of the digital age, Startling by Each Step tempers that trend by slowing things down and suggesting a yearning for the individual＇s temporal sovereignty; finally, the causal loop innate in this genre necessitates a transgression of historical linearity which often leads to unintended catastrophes and results in a prevailing sense of historical determinism. However, in Startling by Each Step a Heidegger-like resolute struggle for finite freedom within time delivers from the recognition of one＇s fateful destiny.
