[Objectives]To explore the action mechanism of Mongolian medicine Rhododendron micranthum Turcz.on lung cancer by network pharmacology.[Methods]Based on the high-throughput experiment and reference database(HERB)of tr...[Objectives]To explore the action mechanism of Mongolian medicine Rhododendron micranthum Turcz.on lung cancer by network pharmacology.[Methods]Based on the high-throughput experiment and reference database(HERB)of traditional Chinese medicine,component target database(Swiss ADME),small molecule drug target prediction online platform(SWISS Target Prediction),human gene business card database(GENECARD),the database of genes and mutation sites related to human diseases(DISGENET)and other databases,the target genes of drugs and diseases were screened out.Venny software was used for obtaining the target intersection of active components of the Mongolian medicine R.micranthum Turcz.and the lung cancer,a CytoNCA plug-in in cytoscape 3.10.0 software was used for screening candidate core target genes,and related effective components were obtained in a reverse direction.A drug-active ingredient-gene-disease regulation network was established,a protein-protein interaction(PPI)network was established by means of the STRING database to screen core genes,and common targets were screened by the David database.Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used for enrichment analysis.[Results]There were 13 effective components of Mongolian medicine R.micranthum Turcz.for treating lung cancer and 115 drug disease intersection target genes.Core genes affecting the disease included SRC,HSP90AB1,EGFR,AKT1,and ERBB2.GO functional enrichment analysis involved 462 items of biological processes,64 items of cellular components and 126 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out cancer pathways,endocrine resistance,PI3K-Akt signaling pathways,proteoglycans in cancer and other signaling pathways.[Conclusions]Mongolian medicine R.micranthum Turcz.can inhibit the proliferation of lung cancer cells from multiple targets and pathways,and the results of network pharmaceutical analysis provide a theoretical basis for further experimental research.展开更多
基金Supported by Inner Mongolia Autonomous Region Department of Education Science and Technology Leading Talents and Innovation Team Building Project and Inner Mongolia Natural Science Foundation Project(2024FX36)Key Research Project of Science and Technology in Colleges and Universities of Inner Mongolia Autonomous Region(NJZZ21029).
文摘[Objectives]To explore the action mechanism of Mongolian medicine Rhododendron micranthum Turcz.on lung cancer by network pharmacology.[Methods]Based on the high-throughput experiment and reference database(HERB)of traditional Chinese medicine,component target database(Swiss ADME),small molecule drug target prediction online platform(SWISS Target Prediction),human gene business card database(GENECARD),the database of genes and mutation sites related to human diseases(DISGENET)and other databases,the target genes of drugs and diseases were screened out.Venny software was used for obtaining the target intersection of active components of the Mongolian medicine R.micranthum Turcz.and the lung cancer,a CytoNCA plug-in in cytoscape 3.10.0 software was used for screening candidate core target genes,and related effective components were obtained in a reverse direction.A drug-active ingredient-gene-disease regulation network was established,a protein-protein interaction(PPI)network was established by means of the STRING database to screen core genes,and common targets were screened by the David database.Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used for enrichment analysis.[Results]There were 13 effective components of Mongolian medicine R.micranthum Turcz.for treating lung cancer and 115 drug disease intersection target genes.Core genes affecting the disease included SRC,HSP90AB1,EGFR,AKT1,and ERBB2.GO functional enrichment analysis involved 462 items of biological processes,64 items of cellular components and 126 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out cancer pathways,endocrine resistance,PI3K-Akt signaling pathways,proteoglycans in cancer and other signaling pathways.[Conclusions]Mongolian medicine R.micranthum Turcz.can inhibit the proliferation of lung cancer cells from multiple targets and pathways,and the results of network pharmaceutical analysis provide a theoretical basis for further experimental research.
