Objectives To explore whether the angiotensin Ι-converting enzyme(ACE) I/D(insertion/deletion) polymorphism is associated with the susceptibility to high altitude pulmonary edema(HAPE) in the Han Chinese. Methods One...Objectives To explore whether the angiotensin Ι-converting enzyme(ACE) I/D(insertion/deletion) polymorphism is associated with the susceptibility to high altitude pulmonary edema(HAPE) in the Han Chinese. Methods One hundred and forty-seven HAPE-p(HAPE patients) and 193 HAPE-r(HAPE resistants) were enrolled from the Yushu earthquake reconstruction workers in Qinghai province where the altitude is over 3 500 m above sea level. Blood samples were collected from each of the HAPE-p and HAPE-r groups. Information about physiological phenotypes was obtained via fieldwork investigation. The ACE-I/D polymorphism in HAPE-p and HAPE-r was detected by polymerase chain reaction(PCR). Results The SaO2 was significantly lower while HR was significant1y higher in HAPE-p group than those in HAPE-r group. The genotype frequencies of ACE-I/D for II, ID, DD in HAPE-r and HAPE-p groups were 0.430, 0.446, 0.124 and 0.435, 0.469, 0.095, respectively, the allelic frequencies of I and D were 0.650, 0.350 and 0.670, 0.330, respectively. The OR of ID, DD and D alleles relative to II for HAPE was 0.961(0.610~1.514), 1.322(0.634~2.758) and 1.080(0.783~1.489). There was no significant difference of the genotypic and the allelic frequencies in ACE-I/D polymorphism between HAPE-p and HAPE-r groups. Conclusions There is no relation between ACE-I/D polymorphism and HAPE in the Han Chinese.展开更多
Neuroglobin(Ngb) is a respiratory protein that is preferentially expressed in brain of mouse and man.In this article,Tibetan antelope,living at altitude of 3 000~5 000 m for millions of years,was selected as the mode...Neuroglobin(Ngb) is a respiratory protein that is preferentially expressed in brain of mouse and man.In this article,Tibetan antelope,living at altitude of 3 000~5 000 m for millions of years,was selected as the model of hypoxia-tolerant adaptation species.Using reverse transcription polymerase chain reaction(RT-PCR) and Western blot techniques,expression of Ngb gene was amplified and analyzed in antelope brain tissue.Our results showed that Ngb homology protein in Tibetan antelope was identified with more sequence similarity with cattle(96%),sheep(95%),and human(95%).We detected that there were some mutations occurred in the Open Reading Frame of Ngb in Tibetan antelope compared with sheep.Phylogenetic analysis of Ngb chain showed that it was closer to cattle than the others.This study suggests possible roles of central nervous system enriched Ngb in adaptation of Tibetan antelope to extremely high altitude.展开更多
Energy metabolism plays an important role in life survival for species living in high altitude hypoxia condition.Air-breathing organisms require oxygen to create energy.Tibetans are the well-adapted highlanders in Qin...Energy metabolism plays an important role in life survival for species living in high altitude hypoxia condition.Air-breathing organisms require oxygen to create energy.Tibetans are the well-adapted highlanders in Qinghai-Tibetan Plateau.It was thought that different metabolic approaches could lead to different adaptation traits to high altitude hypoxia.Recently identified hypoxia inducible factors pathway regulators,endothelial PAS domain protein1(EPAS1)/HIF-2α and PPARA,were involved in decreasing hemoglobin concentrations in Tibetans.Because EPAS1 and PPARA also modulated the energy metabolism during hypoxia,we hypothesized that positive selected EPAS1 and PPARA genes were also involved in unique energy metabolisms in Tibetans.In this brief review,we take a look into genetic determinations to energy metabolisms for hypoxia adaptations traits in Tibetans and mal-adaptive conditions such as high altitude diseases.展开更多
基金supported by research grants including National Science Foundation of China(31160232)National Basic Research Program of China(2012CB518200)+1 种基金Program of International S&T Cooperation of China(2011DFA32720)Natural Science Foundation of Qinghai(2011-Z-919Q)
文摘Objectives To explore whether the angiotensin Ι-converting enzyme(ACE) I/D(insertion/deletion) polymorphism is associated with the susceptibility to high altitude pulmonary edema(HAPE) in the Han Chinese. Methods One hundred and forty-seven HAPE-p(HAPE patients) and 193 HAPE-r(HAPE resistants) were enrolled from the Yushu earthquake reconstruction workers in Qinghai province where the altitude is over 3 500 m above sea level. Blood samples were collected from each of the HAPE-p and HAPE-r groups. Information about physiological phenotypes was obtained via fieldwork investigation. The ACE-I/D polymorphism in HAPE-p and HAPE-r was detected by polymerase chain reaction(PCR). Results The SaO2 was significantly lower while HR was significant1y higher in HAPE-p group than those in HAPE-r group. The genotype frequencies of ACE-I/D for II, ID, DD in HAPE-r and HAPE-p groups were 0.430, 0.446, 0.124 and 0.435, 0.469, 0.095, respectively, the allelic frequencies of I and D were 0.650, 0.350 and 0.670, 0.330, respectively. The OR of ID, DD and D alleles relative to II for HAPE was 0.961(0.610~1.514), 1.322(0.634~2.758) and 1.080(0.783~1.489). There was no significant difference of the genotypic and the allelic frequencies in ACE-I/D polymorphism between HAPE-p and HAPE-r groups. Conclusions There is no relation between ACE-I/D polymorphism and HAPE in the Han Chinese.
基金supported by the"973"National Basic Research Program(2012CB51820500)National Natural Science Foundation of China(NSFC,31160219).
文摘Neuroglobin(Ngb) is a respiratory protein that is preferentially expressed in brain of mouse and man.In this article,Tibetan antelope,living at altitude of 3 000~5 000 m for millions of years,was selected as the model of hypoxia-tolerant adaptation species.Using reverse transcription polymerase chain reaction(RT-PCR) and Western blot techniques,expression of Ngb gene was amplified and analyzed in antelope brain tissue.Our results showed that Ngb homology protein in Tibetan antelope was identified with more sequence similarity with cattle(96%),sheep(95%),and human(95%).We detected that there were some mutations occurred in the Open Reading Frame of Ngb in Tibetan antelope compared with sheep.Phylogenetic analysis of Ngb chain showed that it was closer to cattle than the others.This study suggests possible roles of central nervous system enriched Ngb in adaptation of Tibetan antelope to extremely high altitude.
基金supported by the"973"National Basic Research Program(2012CB51820500)National Natural Science Foundation of China(NSFC,31160219,81160243,31160232,81160012)Qinghai University Medical School Youth and Middle Scientist Cultura Project in 2012
文摘Energy metabolism plays an important role in life survival for species living in high altitude hypoxia condition.Air-breathing organisms require oxygen to create energy.Tibetans are the well-adapted highlanders in Qinghai-Tibetan Plateau.It was thought that different metabolic approaches could lead to different adaptation traits to high altitude hypoxia.Recently identified hypoxia inducible factors pathway regulators,endothelial PAS domain protein1(EPAS1)/HIF-2α and PPARA,were involved in decreasing hemoglobin concentrations in Tibetans.Because EPAS1 and PPARA also modulated the energy metabolism during hypoxia,we hypothesized that positive selected EPAS1 and PPARA genes were also involved in unique energy metabolisms in Tibetans.In this brief review,we take a look into genetic determinations to energy metabolisms for hypoxia adaptations traits in Tibetans and mal-adaptive conditions such as high altitude diseases.